DE769144T1 - Test für inhibitoren von dp-1 und anderen dp-proteinen - Google Patents
Test für inhibitoren von dp-1 und anderen dp-proteinenInfo
- Publication number
- DE769144T1 DE769144T1 DE0769144T DE95923486T DE769144T1 DE 769144 T1 DE769144 T1 DE 769144T1 DE 0769144 T DE0769144 T DE 0769144T DE 95923486 T DE95923486 T DE 95923486T DE 769144 T1 DE769144 T1 DE 769144T1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- phosphorylation
- state
- assay
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract 14
- 102000004169 proteins and genes Human genes 0.000 title claims abstract 14
- 239000003112 inhibitor Substances 0.000 title 1
- 230000026731 phosphorylation Effects 0.000 claims abstract 11
- 238000006366 phosphorylation reaction Methods 0.000 claims abstract 11
- 238000003556 assay Methods 0.000 claims abstract 10
- 239000003795 chemical substances by application Substances 0.000 claims 7
- 101000870232 Tenebrio molitor Diuretic hormone 1 Proteins 0.000 claims 6
- 239000012634 fragment Substances 0.000 claims 4
- 238000012544 monitoring process Methods 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 3
- 230000002401 inhibitory effect Effects 0.000 claims 3
- 230000002163 immunogen Effects 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 230000009822 protein phosphorylation Effects 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 230000000865 phosphorylative effect Effects 0.000 claims 1
- 229920002401 polyacrylamide Polymers 0.000 claims 1
- 230000003405 preventing effect Effects 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000015699 E2F1 Transcription Factor Human genes 0.000 abstract 1
- 108010063774 E2F1 Transcription Factor Proteins 0.000 abstract 1
- 108090001097 Transcription Factor DP1 Proteins 0.000 abstract 1
- 102000004853 Transcription Factor DP1 Human genes 0.000 abstract 1
- 230000006369 cell cycle progression Effects 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Claims (11)
1. Assay für ein potentielles Wachstums-verhinderndes,
-hemmendes- oder -förderndes Mittel, welcher Assay umfaßt:
(i) Zusammenbringen des Mittels mit einer Zelle, die ein DP-Protein enthält; und
(ii) Beobachten des Phosphorylierungs-Stadiums des DP-Proteins.
2. Assay für ein potentielles Wachstums-verhinderndes, -hemmendes- oder -förderndes Mittel, welcher Assay umfaßt:
(i) Bereitstellen eines Extrakts aus eine Zelle, welche ein DP-Protein in einem hypophosphorylierten Stadium enthält;
(ii) Zusammenbringen des Extrakts mit dem Mittel; und
(iii) Beobachten des Phosphorylierungs-Stadiums des DP-Proteins.
3. Assay für ein potentielles Wachstums-verhinderndes, -hemmendes- oder -förderndes Mittel, welcher Assay umfaßt:
(i) Bereitstellen eines Extrakts aus eine Zelle, welche ein DP-Protein in einem phosphorylierten Stadium enthält;
(ii) Zusammenbringen des Extrakts mit dem Mittel; und
(iii) Beobachten des Phosphorylierungs-Stadiums des DP-Proteins.
4. Assay für ein potentielles Modulationsmittels der
DP-Protein-Phosphorylierung, welcher Assay umfaßt:
(i) Zusammenbringen eines Mediums, welches ein DP-Protein in einem hypophosphorylierten oder phosphorylierten Stadium
und ein phosphorylierendes oder dephosphorylierendes Enzym
enthält, mit dem Mittel; und
(ii) Beobachten des Phosphorylierungs-Stadiums des
DP-Proteins.
5. Assay nach einem der vorangegangenen Ansprüche, worin die
Beobachtung des DP-Protein-Phosphorylierungs-Stadiums das
Messen der Menge an 32P im DP-Protein, das Messen des
Grades der Bindung an DP-I des Antikörpers Anti-DP-l(D)
oder das Messen der Mobilität des DP-Proteins auf einem SDS/Polyacrylamid-Gel umfaßt.
6. Monoklonaler Antikörper, gewinnbar durch Verwenden eines Peptids entsprechend der C-terminalen Region von Seq. ID
Nr. 2 als einem Immunogen, welcher Antikörper DP-I in seinem hypophosphorylierten Zustand erkennt, doch weniger
gut an DP-I im phosphorylierten Stadium bindet.
7. Antikörper nach Anspruch 6, welcher spezifisch für (oder worin das Immunogen) ein Peptid ist entsprechend den Resten
385 bis 400 von DP-I.
8. Monoklonaler Antikörper, welcher zur Bindung an einen Abschnitt oder ein Fragment eines DP-Proteins fähig ist,
der oder das eine Phosphorylierungs-Stelle umfaßt.
9. Antikörper nach einem der Ansprüche 6 bis 8, welcher an einen Abschnitt von DP-I bindet und Anti-DP-1(D) ist.
10. Fragment eines eine Phosphorylierungs-Stelle enthaltenden
DP-Proteins, welches phosphoryliert oder nicht phosphoryliert ist.
11. Fragment nach Anspruch 10, welches ein Fragment von DP-I
ist und die Phosphorylierungs-Stelle Thr388 oder Ser391
enthält.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9413327A GB9413327D0 (en) | 1994-07-01 | 1994-07-01 | Assay for inhibitors of dp-1 |
| PCT/GB1995/001567 WO1996001425A2 (en) | 1994-07-01 | 1995-07-03 | Assay for inhibitors of dp-1 and other dp proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE769144T1 true DE769144T1 (de) | 1997-07-10 |
Family
ID=10757696
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69513025T Expired - Fee Related DE69513025T2 (de) | 1994-07-01 | 1995-07-03 | Test für inhibitoren von dp-1 und anderen dp-proteinen |
| DE0769144T Pending DE769144T1 (de) | 1994-07-01 | 1995-07-03 | Test für inhibitoren von dp-1 und anderen dp-proteinen |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69513025T Expired - Fee Related DE69513025T2 (de) | 1994-07-01 | 1995-07-03 | Test für inhibitoren von dp-1 und anderen dp-proteinen |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5871901A (de) |
| EP (1) | EP0769144B1 (de) |
| JP (1) | JPH10502252A (de) |
| AT (1) | ATE186119T1 (de) |
| AU (1) | AU698473B2 (de) |
| CA (1) | CA2193091A1 (de) |
| DE (2) | DE69513025T2 (de) |
| DK (1) | DK0769144T3 (de) |
| FI (1) | FI965289A0 (de) |
| GB (1) | GB9413327D0 (de) |
| NO (1) | NO317397B1 (de) |
| NZ (1) | NZ288712A (de) |
| WO (1) | WO1996001425A2 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6127505A (en) * | 1995-02-02 | 2000-10-03 | Simula Inc. | Impact resistant polyurethane and method of manufacture thereof |
| US6159691A (en) * | 1996-05-15 | 2000-12-12 | Prolifix Limited | Assay for a putative regulator of cell cycle progression |
| GB9610195D0 (en) * | 1996-05-15 | 1996-07-24 | Medical Res Council | Assay |
| AU4321697A (en) * | 1996-09-30 | 1998-04-24 | Kyowa Hakko Kogyo Co. Ltd. | E2f activity inhibitory compounds |
| US6387649B1 (en) | 1996-09-30 | 2002-05-14 | Prolifix Limited | Assay for a regulator of cell cycle progression |
| EP1068335A2 (de) * | 1998-04-09 | 2001-01-17 | E.I. Dupont De Nemours And Company | Zellzyklus-regulierende proteine |
| GB0308711D0 (en) * | 2003-04-15 | 2003-05-21 | Univ The Glasgow | Assay methods |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0669976B1 (de) * | 1992-10-29 | 1999-06-16 | Medical Research Council | Testreihenanalyse-Verfahren mit transkriptionsfaktor DP-1 |
| WO1994018324A2 (en) * | 1993-02-12 | 1994-08-18 | The Salk Institute For Biological Studies | Phospho-specific antibodies against transcription factors, more specifically against a creb derived peptide |
-
1994
- 1994-07-01 GB GB9413327A patent/GB9413327D0/en active Pending
-
1995
- 1995-07-03 US US08/602,846 patent/US5871901A/en not_active Expired - Fee Related
- 1995-07-03 AU AU28036/95A patent/AU698473B2/en not_active Ceased
- 1995-07-03 CA CA002193091A patent/CA2193091A1/en not_active Abandoned
- 1995-07-03 JP JP8503745A patent/JPH10502252A/ja active Pending
- 1995-07-03 WO PCT/GB1995/001567 patent/WO1996001425A2/en active IP Right Grant
- 1995-07-03 AT AT95923486T patent/ATE186119T1/de not_active IP Right Cessation
- 1995-07-03 DE DE69513025T patent/DE69513025T2/de not_active Expired - Fee Related
- 1995-07-03 EP EP95923486A patent/EP0769144B1/de not_active Expired - Lifetime
- 1995-07-03 DE DE0769144T patent/DE769144T1/de active Pending
- 1995-07-03 DK DK95923486T patent/DK0769144T3/da active
- 1995-07-03 NZ NZ288712A patent/NZ288712A/en not_active IP Right Cessation
-
1996
- 1996-12-27 NO NO19965584A patent/NO317397B1/no not_active IP Right Cessation
- 1996-12-31 FI FI965289A patent/FI965289A0/fi not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2803695A (en) | 1996-01-25 |
| CA2193091A1 (en) | 1996-01-18 |
| FI965289A (fi) | 1996-12-31 |
| US5871901A (en) | 1999-02-16 |
| JPH10502252A (ja) | 1998-03-03 |
| DE69513025D1 (de) | 1999-12-02 |
| NZ288712A (en) | 1998-09-24 |
| NO317397B1 (no) | 2004-10-25 |
| EP0769144A2 (de) | 1997-04-23 |
| DE69513025T2 (de) | 2000-04-13 |
| NO965584L (no) | 1997-02-28 |
| DK0769144T3 (da) | 2000-05-08 |
| WO1996001425A2 (en) | 1996-01-18 |
| FI965289A0 (fi) | 1996-12-31 |
| AU698473B2 (en) | 1998-10-29 |
| WO1996001425A3 (en) | 1996-03-14 |
| NO965584D0 (no) | 1996-12-27 |
| EP0769144B1 (de) | 1999-10-27 |
| ATE186119T1 (de) | 1999-11-15 |
| GB9413327D0 (en) | 1994-08-24 |
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