DE69936363D1 - DNAs die für neue fusions-Proteine kodieren und Verfahren zur Herstellung von nützlichen Polypeptiden durch Expression der DNAs - Google Patents

DNAs die für neue fusions-Proteine kodieren und Verfahren zur Herstellung von nützlichen Polypeptiden durch Expression der DNAs

Info

Publication number
DE69936363D1
DE69936363D1 DE69936363T DE69936363T DE69936363D1 DE 69936363 D1 DE69936363 D1 DE 69936363D1 DE 69936363 T DE69936363 T DE 69936363T DE 69936363 T DE69936363 T DE 69936363T DE 69936363 D1 DE69936363 D1 DE 69936363D1
Authority
DE
Germany
Prior art keywords
dnas
expression
methods
fusion proteins
producing useful
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69936363T
Other languages
English (en)
Other versions
DE69936363T2 (de
Inventor
Seiji Sato
Naohiko Higashikuni
Toshiyuki Kudo
Masaakii Kondo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Itoham Foods Inc
Original Assignee
Itoham Foods Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Itoham Foods Inc filed Critical Itoham Foods Inc
Publication of DE69936363D1 publication Critical patent/DE69936363D1/de
Application granted granted Critical
Publication of DE69936363T2 publication Critical patent/DE69936363T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/655—Somatostatins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69936363T 1998-03-31 1999-03-31 DNAs die für neue fusions-Proteine kodieren und Verfahren zur Herstellung von nützlichen Polypeptiden durch Expression der DNAs Expired - Fee Related DE69936363T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP8733998 1998-03-31
JP8733998 1998-03-31

Publications (2)

Publication Number Publication Date
DE69936363D1 true DE69936363D1 (de) 2007-08-09
DE69936363T2 DE69936363T2 (de) 2008-03-13

Family

ID=13912120

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69936363T Expired - Fee Related DE69936363T2 (de) 1998-03-31 1999-03-31 DNAs die für neue fusions-Proteine kodieren und Verfahren zur Herstellung von nützlichen Polypeptiden durch Expression der DNAs

Country Status (3)

Country Link
US (1) US6506595B2 (de)
EP (1) EP0955370B1 (de)
DE (1) DE69936363T2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001068884A1 (fr) 2000-03-14 2001-09-20 Itoham Foods Inc. Procede de preparation d'un polypeptide comprenant une liaison disulfure
DK1354056T3 (en) * 2000-10-10 2015-02-23 Danisco Us Inc IMPROVED secretion of a polypeptide by a microorganism
US7825227B2 (en) * 2002-05-09 2010-11-02 Prolexys Pharmaceuticals, Inc. Method for purification of a protein complex and identification of its components
JP5126879B2 (ja) * 2007-07-13 2013-01-23 独立行政法人海洋研究開発機構 新規ｄｎａ断片およびそれを含む組換えベクター、それらによって形質転換された形質転換体、ならびにそれらの利用
EP2128252A4 (de) * 2008-02-12 2011-03-16 Ils Inc Fusioniertes protein mit insulinvorläufer vom überexpressions- und sekretionstyp, dafür kodierende dna und verfahren zur insulinerzeugung
US20190352365A1 (en) * 2017-01-18 2019-11-21 Savior Lifetec Corporation Expression construct and method for producing proteins of interest

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3586386T2 (de) 1984-10-05 1993-01-14 Genentech Inc Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.
US5459065A (en) * 1985-02-19 1995-10-17 Utah State University Foundation Process for the degradation of coal tar and its constituents by Phanerochaete chrysosporium
ZA90533B (en) * 1989-02-01 1990-10-31 Shionogi & Co A method for the production of glucagon
DE3923963A1 (de) * 1989-07-20 1991-01-31 Behringwerke Ag Muteine des menschlichen erythropoetins, ihre herstellung und ihre verwendung
JPH03251185A (ja) 1990-02-28 1991-11-08 Juzo Udaka 耐熱性β―アミラーゼの製造方法
US5821088A (en) 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
ATE207957T1 (de) * 1992-02-19 2001-11-15 Oregon State Produktion virus-resistenter pflanzen durch einführung von nicht-translatierbarer viraler plus-strang-rna
JPH0638741A (ja) 1992-03-31 1994-02-15 Kumamoto Pref Gov ヒラメ成長ホルモンの製造法
US5532142A (en) 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
JP3489865B2 (ja) 1993-02-22 2004-01-26 住友製薬株式会社 ヒト成長ホルモンの製造法
JP3164181B2 (ja) 1993-03-01 2001-05-08 ヒゲタ醤油株式会社 新規発現ベクタ−、該発現ベクタ−を保有する微生物、該微生物を用いる有用物質の製造法
ATE216723T1 (de) * 1996-02-13 2002-05-15 Japan Chem Res Hormones de croissance humaines mutantes et leur utilisation
US5686418A (en) * 1996-04-08 1997-11-11 Biomeasure Incorporated Prolonging survival of transplanted pancreatic cells
JP3251185B2 (ja) 1996-11-14 2002-01-28 鬼怒川ゴム工業株式会社 自動車用ウィンドモール及びその製造方法

Also Published As

Publication number Publication date
EP0955370A2 (de) 1999-11-10
EP0955370B1 (de) 2007-06-27
EP0955370A3 (de) 2000-12-20
DE69936363T2 (de) 2008-03-13
US20010021515A1 (en) 2001-09-13
US6506595B2 (en) 2003-01-14

Similar Documents

Publication Publication Date Title
ATE309350T1 (de) Verfahren zur herstellung von polypeptiden mit geeigneter glykosilierung
DE69401001D1 (de) Verfahren zur herstellung von keramischen pulvern im nanobereich
DE69632887D1 (de) Verfahren zur zweckmässige Herstellung von Transglutaminase durch DNA-Rekombination
DE69635076D1 (de) Verfahren zur kontrollierung der sialylierung von durch säugetierzellkulturhergestellten protein
ATE353921T1 (de) Chimäre polypeptide, verfahren zur herstellung und verwendung dafür
DE69809382D1 (de) Verfahren und Zusammensetzungen zur Herstellung von Kupferoberflächen mit verbesserter Haftung und damit hergestellte Gegenstände
ATE230939T1 (de) Verfahren zur herstellung von plastifizierte proteinhaltige stoffe und zusammensetzungen daraus
DE69636831D1 (de) Verfahren zur herstellung heteromultimerer polypeptide
DE69832209D1 (de) Verfahren zur Herstellung von 3,3-Dimethylbutyraldehyd durch Oxidation von 3,3-Dimethylbutanol
ATE237008T1 (de) Verfahren zur elektrolytischen herstellung von metallen
DE69822827D1 (de) Verfahren zur herstellung von mehrkomponentigen und mehrlagigen vliesstoffen
DE69807545D1 (de) Verfahren zur Herstellung von Hohlkörpern aus Verbundmaterial
DE69522517D1 (de) Verfahren zur Herstellung von verstärkten Steinplatten
DE69506020D1 (de) Verfahren zur Herstellung von Verbundmembranen
DE69113025D1 (de) Drei neue nichtpolyglutamisierende deazaaminopterine und verfahren zur herstellung.
DE69621912D1 (de) Verfahren zur Herstellung von Silica-Ceriumoxidkompositteilchen
DE60223742D1 (de) Verfahren zur Herstellung von zusammengesetzten Farbstoff- Partikeln
DE69815734D1 (de) Verfahren zur Herstellung von hohlen Präzisionskörpern aus Verbundwerkstoff
DE69808569D1 (de) Verfahren zur Herstellung von keramischen Pulvern
ATE239764T1 (de) Verfahren zur verbesserung der aromahaltenden eigenschaft von behältern aus polyester-polyamid- mischungen für ozonwasser
DE69830556D1 (de) Verfahren zur Bestimmung von glykosylierten Proteinen
DE69936363D1 (de) DNAs die für neue fusions-Proteine kodieren und Verfahren zur Herstellung von nützlichen Polypeptiden durch Expression der DNAs
ATE258558T1 (de) Lantibiotikum verwandt mit actagardine, verfahren zur herstellung und verwendung desselben
DE19581874T1 (de) Verfahren zur Herstellung von Verbundpolstern
DE69828583D1 (de) Verfahren zur herstellung von zement

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee