DE699128C - ruesen hormones from body fluids - Google Patents

ruesen hormones from body fluids

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Publication number
DE699128C
DE699128C DE1930699128D DE699128DD DE699128C DE 699128 C DE699128 C DE 699128C DE 1930699128 D DE1930699128 D DE 1930699128D DE 699128D D DE699128D D DE 699128DD DE 699128 C DE699128 C DE 699128C
Authority
DE
Germany
Prior art keywords
hormones
body fluids
ruesen
hormone
urine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE1930699128D
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German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Pharma AG
Original Assignee
Schering AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Schering AG filed Critical Schering AG
Application granted granted Critical
Publication of DE699128C publication Critical patent/DE699128C/en
Expired legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

Verfahren zur Anreicherung und Gewinnung von-Keimdrüsenhormonen aus Körperflüssigkeiten In der Patentschrift 671 043 ist ein Verfahren zur Reinigung, Anreicherung und Gewinnung von Hormonen beschrieben,- zach dem man die das Hormon enthaltenden Lösungen mit einem Salz sättigt und dann das Salz partiell auskristallisieren läßt.Process for the enrichment and extraction of gonadal hormones Body fluids In the patent specification 671 043 a method for cleaning, Enrichment and production of hormones described - zach which one the the hormone containing solutions is saturated with a salt and then partially crystallize out the salt leaves.

Es wurde nun gefunden, daß man dieses Aussalzverfahren insbesondere zur Anreicherung von Keimdrüsenhormonen vorteilhaft in der Weise vornimmt, daß man die das Hormon enthaltende Lösung vor dem. Aussahen ansäuert. Dieses vorherige Ansäuern hat die Vorteile, daß eine leichtere Aussalzbarkeit der Hormone ermöglicht wird, da diese in der angesäuerten Lösung schwerer löslich sind, und ferner, daß eine geringere Verunreinigung durch die Aussalzmasse eintritt, da durch die schwerere Löslichkeit der Hormone in der angesäuerten Lösung nur .eine Sättigung bis zu 6o bis 8o% des Sättigungsgrades notwendig ist und man also ein reineres Hormonprodukt erhält. Zum Ansäuern eignen sich Salzsäure, Schwefelsäure, Essigsäure o. dgl. Zum Aussalzen eignen sich die im. Hauptpatent .angegebenen Salze.It has now been found that this salting out process in particular for the enrichment of gonadal hormones advantageously in such a way that one the solution containing the hormone before. Look acidic. This previous acidification has the advantage that the hormones can be salted out more easily, since these are more difficult to dissolve in the acidified solution, and further that a less contamination occurs due to the salting-out compound, as the heavier one Solubility of the hormones in the acidified solution only. A saturation up to 6o up to 8o% of the saturation level is necessary and you get a purer hormone product receives. Hydrochloric acid, sulfuric acid, acetic acid or the like are suitable for acidification The im. Main patent. Specified salts.

Beispiel i ioo 1 Harn gravider Frauen mit einem Gehalt von etwa 12 000 ME. pro i weiblichen Cyclushormons werden mit verd. H Cl bis zur kongosauren Reaktion angesäuert und der Harn.' dann bis zu 8o% des -Sättigungsgrades. mit Ammonsulfat versetzt. Nach kühler Aufbewahrung über Nacht wird das Aussalzprodukt, das etwa i ooo ooo M.E. an Hormon enthält, abgetrennt und das Hormon daraus nach bekannten Methoden isoliert. Beispiel 2 i oo 1 Männerharn werden mit werd. H Cl bis zur kongosauren Reaktion angesäuert und der Harn dann bis zu 8o% des Sättigungsgrades mit Natriumsulfat gesättigt. Nach mehrstündigem. Stehen wird der feste Anteil, welcher die gesamte Menge des im- Harn enthaltenen Keimdrüsenhormons enthält, abgetrennt und aus demselben das männliche Keimdrüsenhormon in bekannter Weise extrahiert.Example 100 1 urine of pregnant women with a content of about 12,000 ME. per i female cycle hormones are acidified with dil. H Cl until the Congo-acidic reaction and the urine. ' then up to 8o% of the degree of saturation. mixed with ammonium sulfate. After storing in a cool place overnight, the salting-out product, which contains about 1,000,000 MU of hormone, is separated off and the hormone is isolated therefrom by known methods. Example 2 i oo 1 male urine will be with be. H Cl acidified to the Congo acid reaction and the urine then saturated with sodium sulfate up to 80% of the degree of saturation. After several hours. The solid portion, which contains the entire amount of the gonad hormone contained in the urine, is separated off and the male gonad hormone is extracted from it in a known manner.

Claims (1)

PATLNTANSPIZUCH: Verfahren zur Anreicherung und Gewinnung von Keimdrüsenhormonen aus Körperflüssigkeiten gemäß Patent 671 o43, dadurch gekennzeichnet, daß die hormonhaltigen Flüssigkeiten hier vor dem Aussalzen angesäuert werden.PATLNTANSPIZUCH: Process for the enrichment and production of gonadal hormones from body fluids according to patent 671 043, characterized in that the hormone-containing Liquids are acidified here before salting out.
DE1930699128D 1930-10-28 1930-10-28 ruesen hormones from body fluids Expired DE699128C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE699128T 1930-10-28

Publications (1)

Publication Number Publication Date
DE699128C true DE699128C (en) 1940-11-22

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ID=6608342

Family Applications (1)

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DE1930699128D Expired DE699128C (en) 1930-10-28 1930-10-28 ruesen hormones from body fluids

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Country Link
DE (1) DE699128C (en)

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