DE69835207D1 - Synthese von oligonukleotiden - Google Patents

Synthese von oligonukleotiden

Info

Publication number
DE69835207D1
DE69835207D1 DE69835207T DE69835207T DE69835207D1 DE 69835207 D1 DE69835207 D1 DE 69835207D1 DE 69835207 T DE69835207 T DE 69835207T DE 69835207 T DE69835207 T DE 69835207T DE 69835207 D1 DE69835207 D1 DE 69835207D1
Authority
DE
Germany
Prior art keywords
oligonucleotide
free
solid support
oligonucleotides
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69835207T
Other languages
English (en)
Inventor
Marek Kwiatkowski
Ulf Landegren
Mats Nilsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quiatech AB
Original Assignee
Quiatech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quiatech AB filed Critical Quiatech AB
Application granted granted Critical
Publication of DE69835207D1 publication Critical patent/DE69835207D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
DE69835207T 1997-05-14 1998-05-14 Synthese von oligonukleotiden Expired - Lifetime DE69835207D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9701783A SE9701783D0 (sv) 1997-05-14 1997-05-14 In situ synthesis of oligonucleotides of inverse orientation
PCT/SE1998/000893 WO1998051698A1 (en) 1997-05-14 1998-05-14 Synthesis of oligonucleotides

Publications (1)

Publication Number Publication Date
DE69835207D1 true DE69835207D1 (de) 2006-08-24

Family

ID=20406926

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69835207T Expired - Lifetime DE69835207D1 (de) 1997-05-14 1998-05-14 Synthese von oligonukleotiden

Country Status (8)

Country Link
US (2) US6313284B1 (de)
EP (1) EP0983288B1 (de)
JP (1) JP2001525821A (de)
AT (1) ATE332911T1 (de)
CA (1) CA2289204A1 (de)
DE (1) DE69835207D1 (de)
SE (1) SE9701783D0 (de)
WO (1) WO1998051698A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9701783D0 (sv) * 1997-05-14 1997-05-14 Marek Kwiatkowski In situ synthesis of oligonucleotides of inverse orientation
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
WO2001038580A2 (en) * 1999-11-26 2001-05-31 Curagen Corporation Nucleic acid probe arrays
WO2001092566A2 (de) * 2000-05-26 2001-12-06 Noxxon Pharma Ag Immobilisierte nukleinsäuren und verwendungen davon
US6932943B1 (en) 2001-01-26 2005-08-23 Third Wave Technologies Nucleic acid synthesizers
US7435390B2 (en) * 2001-01-26 2008-10-14 Third Wave Technologies, Inc. Nucleic acid synthesizers
JP2004533257A (ja) 2001-06-15 2004-11-04 キアテック アクチボラゲット 増幅可能なプローブ
US7345163B2 (en) 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
EP1756307A1 (de) * 2004-05-20 2007-02-28 Trillion Genomics Limited Verwendung massenmarkierter sonden zum nachweis von zielnukleinsäuren mittels massenspektrometrie
US20060068424A1 (en) * 2004-08-13 2006-03-30 Fuji Photo Film Co., Ltd. Biosensor
EP1788875A4 (de) * 2004-08-20 2010-12-15 Dharmacon Inc Neue polynukleotidsynthese durch markierungschemie
US9249460B2 (en) 2011-09-09 2016-02-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
JP2013198425A (ja) * 2012-03-24 2013-10-03 Kanagawa Univ オリゴヌクレオチドの製造方法
CN106460232B (zh) * 2014-05-23 2019-12-24 生捷科技控股公司 用于原位合成的探针阵列的寡核苷酸探针倒转方法
US10695735B2 (en) 2015-08-18 2020-06-30 Centrillion Technology Holdings Corporation Probe inversion process for in situ synthesized probe arrays
WO2017031278A1 (en) * 2015-08-18 2017-02-23 Centrillion Technology Holdings Corporation Probe inversion process for in situ synthesized probe arrays
WO2018102660A1 (en) * 2016-12-02 2018-06-07 Centrillion Technologies, Inc. In situ probe inversion process for constructing probe arrays
US20210032776A1 (en) * 2018-03-21 2021-02-04 Centrillion Technology Holdings Corporation Method and system for fabricating dna sequencing arrays

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU598946B2 (en) * 1987-06-24 1990-07-05 Howard Florey Institute Of Experimental Physiology And Medicine Nucleoside derivatives
SE9003743D0 (sv) 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
US5550215A (en) * 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
SE9701783D0 (sv) * 1997-05-14 1997-05-14 Marek Kwiatkowski In situ synthesis of oligonucleotides of inverse orientation

Also Published As

Publication number Publication date
CA2289204A1 (en) 1998-11-19
WO1998051698A1 (en) 1998-11-19
WO1998051698A8 (en) 1999-06-10
US6313284B1 (en) 2001-11-06
US6790946B2 (en) 2004-09-14
EP0983288B1 (de) 2006-07-12
EP0983288A1 (de) 2000-03-08
US20020051994A1 (en) 2002-05-02
JP2001525821A (ja) 2001-12-11
SE9701783D0 (sv) 1997-05-14
ATE332911T1 (de) 2006-08-15

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