DE69826237D1 - Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease PmeI - Google Patents

Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease PmeI

Info

Publication number
DE69826237D1
DE69826237D1 DE69826237T DE69826237T DE69826237D1 DE 69826237 D1 DE69826237 D1 DE 69826237D1 DE 69826237 T DE69826237 T DE 69826237T DE 69826237 T DE69826237 T DE 69826237T DE 69826237 D1 DE69826237 D1 DE 69826237D1
Authority
DE
Germany
Prior art keywords
cloning
production
restriction endonuclease
endonuclease pmei
pmei
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69826237T
Other languages
English (en)
Other versions
DE69826237T2 (de
Inventor
Zhiyuh Chang
Richard D Morgan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of DE69826237D1 publication Critical patent/DE69826237D1/de
Application granted granted Critical
Publication of DE69826237T2 publication Critical patent/DE69826237T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69826237T 1997-11-24 1998-11-24 Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease PmeI Expired - Lifetime DE69826237T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US976703 1997-11-24
US08/976,703 US5945288A (en) 1997-11-24 1997-11-24 Method for cloning and producing the PmeI restriction endonuclease

Publications (2)

Publication Number Publication Date
DE69826237D1 true DE69826237D1 (de) 2004-10-21
DE69826237T2 DE69826237T2 (de) 2005-09-29

Family

ID=25524373

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69826237T Expired - Lifetime DE69826237T2 (de) 1997-11-24 1998-11-24 Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease PmeI

Country Status (4)

Country Link
US (1) US5945288A (de)
EP (1) EP0931835B1 (de)
JP (1) JP4394762B2 (de)
DE (1) DE69826237T2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6846658B1 (en) * 2000-10-12 2005-01-25 New England Biolabs, Inc. Method for cloning and producing the Msel restriction endonuclease
US7521651B2 (en) * 2003-09-12 2009-04-21 Orbotech Ltd Multiple beam micro-machining system and method
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
WO2005087932A2 (en) * 2003-10-03 2005-09-22 Promega Corporation Vectors for directional cloning
EP2540823A1 (de) 2005-08-04 2013-01-02 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
EP3130673B1 (de) * 2015-08-13 2019-06-12 Centrillion Technology Holdings Corporation Bibliothekkonstruktion mit y-adaptern und schwindenden restriktionsstellen

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200333A (en) * 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
US5196330A (en) * 1991-06-03 1993-03-23 New England Biolabs, Inc. Type ii restriction endonuclease, pme i, obtainable from pseudomonas mendocina and a process for producing the same
US5543308A (en) * 1994-10-18 1996-08-06 New England Biolabs, Inc. Isolated DNA encoding the FSEI restriction endonuclease and related methods for producing the same
US5721126A (en) * 1995-12-08 1998-02-24 New England Biolabs, Inc. Method for cloning and producing the SCaI restriction endonuclease in E. coli

Also Published As

Publication number Publication date
EP0931835A2 (de) 1999-07-28
EP0931835B1 (de) 2004-09-15
JP4394762B2 (ja) 2010-01-06
DE69826237T2 (de) 2005-09-29
JPH11206394A (ja) 1999-08-03
EP0931835A3 (de) 2000-04-12
US5945288A (en) 1999-08-31

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition