DE69817000D1 - Verfahren zur schnellen isolierung von rns - Google Patents
Verfahren zur schnellen isolierung von rnsInfo
- Publication number
- DE69817000D1 DE69817000D1 DE69817000T DE69817000T DE69817000D1 DE 69817000 D1 DE69817000 D1 DE 69817000D1 DE 69817000 T DE69817000 T DE 69817000T DE 69817000 T DE69817000 T DE 69817000T DE 69817000 D1 DE69817000 D1 DE 69817000D1
- Authority
- DE
- Germany
- Prior art keywords
- rns
- quick isolation
- isolation
- quick
- test samples
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 title 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Materials For Photolithography (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/931,981 US5817798A (en) | 1997-09-17 | 1997-09-17 | Rapid RNA isolation procedure in the presence of a transition metal ion |
US931981 | 1997-09-17 | ||
PCT/US1998/019043 WO1999014224A1 (en) | 1997-09-17 | 1998-09-11 | Rapid rna isolation procedure |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69817000D1 true DE69817000D1 (de) | 2003-09-11 |
DE69817000T2 DE69817000T2 (de) | 2004-07-22 |
Family
ID=25461601
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69817000T Expired - Fee Related DE69817000T2 (de) | 1997-09-17 | 1998-09-11 | Verfahren zur schnellen isolierung von rns |
Country Status (8)
Country | Link |
---|---|
US (1) | US5817798A (de) |
EP (1) | EP1017705B1 (de) |
JP (1) | JP2001516763A (de) |
AT (1) | ATE246698T1 (de) |
CA (1) | CA2303398A1 (de) |
DE (1) | DE69817000T2 (de) |
ES (1) | ES2205545T3 (de) |
WO (1) | WO1999014224A1 (de) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
DE19900638C2 (de) * | 1999-01-11 | 2002-12-19 | Max Planck Gesellschaft | Methode zur Isolierung von DNA aus biologischen Materialien |
WO2000044940A2 (en) * | 1999-01-28 | 2000-08-03 | Gen-Probe Incorporated | Nucleic acid sequences for detecting genetic markers for cancer in a biological sample |
DE10004927C2 (de) * | 2000-02-04 | 2003-02-06 | Qiagen Gmbh | Nukleinsäure-Isolierung aus Stuhlproben und anderen biologischen Materialien, die reich an Inhibitoren sind |
CA2399475C (en) * | 2000-02-04 | 2011-04-12 | Qiagen Gmbh | Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors |
US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
EP3617321A3 (de) * | 2006-05-31 | 2020-04-29 | Sequenom, Inc. | Verfahren und zusammensetzungen zur nukleinsäureextraktion und -verstärkung aus einer probe |
US20110027862A1 (en) * | 2009-06-29 | 2011-02-03 | Life Technologies Corporation | Sample stabilization |
EP2574669B1 (de) * | 2011-06-29 | 2018-10-17 | Kabushiki Kaisha Dnaform | Verfahren zur vorbehandlung biologischer proben, verfahren zur erkennung von rna und vorbehandlungssatz |
US9828600B2 (en) | 2013-09-20 | 2017-11-28 | University Of Massachusetts | Compositions and methods for constructing cDNA libraries that allow for mapping the 5′ and 3′ ends of RNAs |
CN115197940A (zh) | 2015-07-14 | 2022-10-18 | 雅培分子公司 | 使用铜-钛氧化物纯化核酸 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4483920A (en) * | 1982-05-17 | 1984-11-20 | Hahnemann University | Immobilization of message RNA directly from cells onto filter material |
IL67576A0 (en) * | 1982-12-28 | 1983-05-15 | Wreschner Daniel Hyam | Carrier sheets of paper and nitrocellulose bearing polyuridylic acid and polythymidylic acid residues and their use in the preparative recovery of mrna |
ATE107654T1 (de) * | 1987-03-02 | 1994-07-15 | Gen Probe Inc | Polykationische träger zur reinigung, trennung und hybridisierung von nukleinsäure. |
US5063162A (en) * | 1987-04-24 | 1991-11-05 | Hoffmann-La Roche Inc. | Process for isolating nucleic acids utilizing protease digestion |
US4843155A (en) * | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
EP0403503A4 (en) * | 1988-02-09 | 1992-06-24 | Memorial Blood Center Of Minneapolis | Nucleic acid isolation |
US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
CA2067711C (en) * | 1991-05-03 | 2000-08-08 | Daniel Lee Woodard | Solid phase extraction purification of dna |
DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
JP2595922B2 (ja) * | 1995-06-09 | 1997-04-02 | 日立化成工業株式会社 | 抗rna結合蛋白質抗体の測定法 |
-
1997
- 1997-09-17 US US08/931,981 patent/US5817798A/en not_active Expired - Lifetime
-
1998
- 1998-09-11 DE DE69817000T patent/DE69817000T2/de not_active Expired - Fee Related
- 1998-09-11 EP EP98944865A patent/EP1017705B1/de not_active Expired - Lifetime
- 1998-09-11 WO PCT/US1998/019043 patent/WO1999014224A1/en active IP Right Grant
- 1998-09-11 CA CA002303398A patent/CA2303398A1/en not_active Abandoned
- 1998-09-11 JP JP2000511773A patent/JP2001516763A/ja not_active Withdrawn
- 1998-09-11 ES ES98944865T patent/ES2205545T3/es not_active Expired - Lifetime
- 1998-09-11 AT AT98944865T patent/ATE246698T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE69817000T2 (de) | 2004-07-22 |
US5817798A (en) | 1998-10-06 |
EP1017705B1 (de) | 2003-08-06 |
WO1999014224A1 (en) | 1999-03-25 |
ATE246698T1 (de) | 2003-08-15 |
JP2001516763A (ja) | 2001-10-02 |
CA2303398A1 (en) | 1999-03-25 |
EP1017705A1 (de) | 2000-07-12 |
ES2205545T3 (es) | 2004-05-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |