DE69738603D1 - Verfahren zur Herstellung von Banken vollständiger cDNAs - Google Patents

Verfahren zur Herstellung von Banken vollständiger cDNAs

Info

Publication number
DE69738603D1
DE69738603D1 DE69738603T DE69738603T DE69738603D1 DE 69738603 D1 DE69738603 D1 DE 69738603D1 DE 69738603 T DE69738603 T DE 69738603T DE 69738603 T DE69738603 T DE 69738603T DE 69738603 D1 DE69738603 D1 DE 69738603D1
Authority
DE
Germany
Prior art keywords
libraries
preparation
complete cdnas
cdnas
complete
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69738603T
Other languages
English (en)
Other versions
DE69738603T2 (de
Inventor
Yoshihide Hayashizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN Institute of Physical and Chemical Research
Original Assignee
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN Institute of Physical and Chemical Research filed Critical RIKEN Institute of Physical and Chemical Research
Application granted granted Critical
Publication of DE69738603D1 publication Critical patent/DE69738603D1/de
Publication of DE69738603T2 publication Critical patent/DE69738603T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69738603T 1996-11-01 1997-10-31 Verfahren zur Herstellung von Banken vollständiger cDNAs Expired - Lifetime DE69738603T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP29150096 1996-11-01
JP29150096A JP3441899B2 (ja) 1996-11-01 1996-11-01 完全長ｃＤＮＡライブラリーの作成方法

Publications (2)

Publication Number Publication Date
DE69738603D1 true DE69738603D1 (de) 2008-05-08
DE69738603T2 DE69738603T2 (de) 2009-04-30

Family

ID=17769693

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69738603T Expired - Lifetime DE69738603T2 (de) 1996-11-01 1997-10-31 Verfahren zur Herstellung von Banken vollständiger cDNAs
DE69731234T Expired - Lifetime DE69731234T2 (de) 1996-11-01 1997-10-31 METHODE FÜR DIE HERSTELLUNG EINER cDNA-BANK AUS KOMPLETTEN cDNAS.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69731234T Expired - Lifetime DE69731234T2 (de) 1996-11-01 1997-10-31 METHODE FÜR DIE HERSTELLUNG EINER cDNA-BANK AUS KOMPLETTEN cDNAS.

Country Status (6)

Country Link
US (1) US6143528A (de)
EP (2) EP1469069B1 (de)
JP (1) JP3441899B2 (de)
CA (1) CA2270414C (de)
DE (2) DE69738603T2 (de)
WO (1) WO1998020122A1 (de)

Families Citing this family (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
WO1998056943A1 (en) 1997-06-12 1998-12-17 Sloan-Kettering Institute For Cancer Research Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
CA2286850A1 (en) 1998-11-06 2000-05-06 Riken Method for producing double-stranded dna whose terminal homopolymer part is eliminated and method for determining nucleotide sequence
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
AU3397800A (en) * 1999-03-19 2000-10-09 Invitrogen Corporation Methods of obtaining full-length nucleic acid sequences using (e. coli) topoisomerase iii and its homologs
WO2001004286A1 (fr) * 1999-07-08 2001-01-18 Helix Research Institute PROCEDE DE CREATION D'UNE BANQUE D'ADNc PLEINE LONGUEUR
EP1261724A4 (de) 2000-02-25 2004-08-18 Invitrogen Corp Topoisomerase linker-vermittelte amplifizierungsverfahren
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1313851B1 (de) 2000-08-21 2006-01-04 Invitrogen Corporation Methoden und reagenzien für molekulares klonieren
US20040166499A1 (en) * 2000-10-05 2004-08-26 Yoshihide Hayashizaki Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers
US20060008817A1 (en) 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
AU2002248173B2 (en) 2000-12-08 2007-04-26 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
MXPA04002817A (es) 2001-09-27 2004-07-05 Pioneer Hi Bred Int Polinucleotidos de fitato y metodos de uso.
MXPA04008071A (es) 2002-02-19 2004-11-26 Dow Agrosciences Llc Nuevas sintasas de poliquetida que producen espinosina.
US7358419B2 (en) 2002-04-08 2008-04-15 Pioneer Hi-Bred International, Inc. Enhanced silk exsertion under stress
US20050175993A1 (en) * 2002-04-12 2005-08-11 Chia-Lin Wei Method for making full-length coding sequence cDNA libraries
US20050250100A1 (en) * 2002-06-12 2005-11-10 Yoshihide Hayashizaki Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
MXPA05001287A (es) 2002-08-06 2005-08-03 Verdia Llc Variantes de amino oxidasa ap1.
US7205128B2 (en) * 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
EP1620557A2 (de) 2003-04-29 2006-02-01 Pioneer Hi-Bred International, Inc. Neue glyphosat-n-acetyltransferase (gat)-gene
AU2004265250C1 (en) 2003-06-23 2008-05-01 Pioneer Hi-Bred International, Inc. Engineering single-gene-controlled staygreen potential into plants
US8222005B2 (en) * 2003-09-17 2012-07-17 Agency For Science, Technology And Research Method for gene identification signature (GIS) analysis
US20060084799A1 (en) * 2003-09-24 2006-04-20 Williams Lewis T Human cDNA clones comprising polynucleotides encoding polypeptides and methods of their use
US20070274988A1 (en) * 2003-10-10 2007-11-29 Five Prime Therapeautics, Inc. Kiaa0779, Splice Variants Thereof, and Methods of Their Use
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
AU2005327983B2 (en) 2004-06-30 2008-08-07 Pioneer Hi-Bred International, Inc. Methods of protecting plants from pathogenic fungi
MXPA06015054A (es) 2004-07-02 2007-08-07 Pioneer Hi Bred Int Polipeptidos antifungales.
EP1831376A1 (de) 2004-12-28 2007-09-12 Pioneer Hi-Bred International, Inc. Verbesserte kornqualität durch eine veränderte expression von samenproteinen
WO2006127310A2 (en) 2005-05-25 2006-11-30 Pioneer Hi-Bred International, Inc. Methods for improving crop plant architecture and yield
EP2730587A3 (de) 2006-02-09 2014-08-27 Pioneer Hi-Bred International, Inc. Gene zur Verbesserung der Stickstoffnutzungseffizienz bei Nutzpflanzen
EP1991047B1 (de) 2006-03-01 2012-09-26 Pioneer Hi-Bred International Inc. Zusammensetzung in zusammenhang mit dem quantitative trait locus 6 (qtl6) in mais und anwendungsverfahren dafür
US20070214515A1 (en) 2006-03-09 2007-09-13 E.I.Du Pont De Nemours And Company Polynucleotide encoding a maize herbicide resistance gene and methods for use
EP2251349A1 (de) 2006-04-19 2010-11-17 Pioneer Hi-Bred International, Inc. Isolierte Polynukleotidmoleküle, welche Mutanten- und Wildtypallelen des D9-Gens aus Mais entsprechen, und Verwendungsmethoden
EP2333088B1 (de) 2006-05-16 2013-08-28 Pioneer Hi-Bred International, Inc. Antimykotische Polypeptide
US20080124707A1 (en) * 2006-06-09 2008-05-29 Agency For Science, Technology And Research Nucleic acid concatenation
US7951995B2 (en) 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
US8367895B2 (en) 2008-01-17 2013-02-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from the family aphididae
US8847013B2 (en) 2008-01-17 2014-09-30 Pioneer Hi Bred International Inc Compositions and methods for the suppression of target polynucleotides from lepidoptera
EP2344640A1 (de) 2008-10-30 2011-07-20 Pioneer Hi-Bred International Inc. Manipulation von glutamin-synthetasen (gs) zur verbesserung der stickstoffnutzungseffizienz und kornausbeute bei höheren pflanzen
US20100210460A1 (en) 2009-02-19 2010-08-19 Pioneer Hi-Bred International, Inc. Blended refuge deployment via manipulation during hybrid seed production
BRPI1014658A2 (pt) 2009-04-14 2019-09-24 Pioneer Hi Bred Int "método para aperfeiçoar a tolerância ao estresse de intrôgenio em uma planta, método para aperfeiçoar a tolerância ao estresse de nitrogênio sob condições com pouco nitrogênio, cassete de expressão, construto, célula vegetal, planta e método de inibição da produção de etileno em uma planta."
WO2011011273A1 (en) 2009-07-24 2011-01-27 Pioneer Hi-Bred International, Inc. The use of dimerization domain component stacks to modulate plant architecture
WO2011017492A2 (en) 2009-08-05 2011-02-10 Pioneer Hi-Bred International, Inc. Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
WO2011022597A1 (en) 2009-08-20 2011-02-24 Pioneer Hi-Bred International, Inc. Functional expression of shuffled yeast nitrate transporter (ynti) in maize to improve nitrate uptake under low nitrate environment
IN2012DN01315A (de) 2009-08-20 2015-06-05 Pioneer Hi Bred Int
BR122018067960B8 (pt) 2009-08-28 2022-12-06 Du Pont Polinucleotídeo isolado, cassete de expressão, método para controlar uma praga de plantas do tipo coleoptera e método para obtenção de uma planta
IN2012DN02408A (de) 2009-10-02 2015-08-21 Pioneer Hi Bred Int
EP2529018B1 (de) 2009-12-30 2016-06-22 Pioneer Hi-Bred International, Inc. Verfahren und zusammensetzungen zur einbringung und regulierten expression von genen in pflanzen
CN102939383B (zh) 2009-12-30 2015-04-29 先锋国际良种公司 用于靶向多核苷酸修饰的方法和组合物
WO2011082304A1 (en) 2009-12-31 2011-07-07 Pioneer Hi-Bred International, Inc. Engineering plant resistance to diseases caused by pathogens
CA2786741A1 (en) 2010-01-06 2011-07-14 Pioneer Hi-Bred International, Inc. Identification of diurnal rhythms in photosynthetic and non-photosynthetic tissues from zea mays and use in improving crop plants
CN102958349B (zh) 2010-06-25 2015-12-02 纳幕尔杜邦公司 用于在玉米中增强玉米大斑病抗性的组合物和方法
AU2011289286B2 (en) 2010-08-13 2015-02-12 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
BR112013003223A2 (pt) 2010-08-23 2016-06-07 Pioneer Hi Bred Int "polinucleotídeo isolado, cassete de expressão, célula hospedeira, micro-organismo, planta ou parte de planta, método de obtenção de uma planta transformada, composição antipatogênica, método para proteger uma planta contra um patógeno ou uso de um polinucleotídeo isolado"
CN107815462A (zh) 2010-11-24 2018-03-20 先锋国际良种公司 芸苔gat事件dp‑073496‑4及其鉴定和/或检测组合物和方法
CA2818918A1 (en) 2010-11-24 2012-05-31 Pioneer Hi-Bred International, Inc. Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
BR112013015515A2 (pt) 2010-12-28 2018-04-24 Pioneer Hi Bred Int molécula de ácido nucleico isolada, construto de dna, célula hospedeira, planta transgênica, semente transformada da planta, polipeptídeo isolado com atividade pesticida, composição, método para controlar uma população de praga de lepidóptero, método para matar uma praga de lepidóptero, método para produzir um polipeptídeo com atividade pesticida, planta que tem incorporado de maneira estável em seu genoma um construto de dna, método para proteger uma planta contra uma praga
US9578880B2 (en) 2011-02-01 2017-02-28 Colorado Wheat Research Foundation, Inc. Acetyl co-enzyme A carboxylase herbicide resistant plants
MX2013009092A (es) 2011-02-11 2013-10-17 Pioneer Hi Bred Int Proteinas insecticidas sinteticas activas contra el gusano de la raiz del maiz.
US8878007B2 (en) 2011-03-10 2014-11-04 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
US9090906B2 (en) 2011-03-30 2015-07-28 Universidad Nacional Autonoma De Mexico Mutant Bacillus thuringiensis cry genes and methods of use
US9677084B2 (en) 2011-04-29 2017-06-13 Pioneer Hi-Bred International, Inc. Down-regulation of a homeodomain-leucine zipper I-class homeobox gene for improved plant performance
GB201107286D0 (en) * 2011-05-03 2011-06-15 Epistem Ltd Nucleic acid preparation
WO2013063344A1 (en) 2011-10-28 2013-05-02 Pioneer Hi-Bred International, Inc. Engineered pep carboxylase variants for improved plant productivity
CN104093842B (zh) 2011-10-31 2016-12-07 先锋国际良种公司 改善植物耐旱性、氮利用效率和产量
US9783814B2 (en) 2012-03-13 2017-10-10 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
BR112014031260A2 (pt) 2012-06-15 2019-08-20 Du Pont métodos e composições que envolvem variantes de als com preferência de substrato nativo
US9347105B2 (en) 2012-06-15 2016-05-24 Pioneer Hi Bred International Inc Genetic loci associated with resistance of soybean to cyst nematode and methods of use
BR112015005389B1 (pt) 2012-09-13 2022-10-18 Indiana University Research And Technology Corporation Molécula de ácido nucleico recombinante, proteína de substrato modificada de uma protease específica de patógeno de planta expressa através do patógeno de planta, vetor, e método para proteger uma planta da infecção por um patógeno de planta que secreta pelo menos uma protease específica
WO2014062544A2 (en) 2012-10-15 2014-04-24 Pioneer Hi-Bred International, Inc. Methods and compositions to enhance activity of cry endotoxins
US20150299718A1 (en) 2012-11-20 2015-10-22 Rajeev Gupta Engineering Plants for Efficient Uptake and Utilization of Urea to Improve
US20140173781A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic wheat plants
WO2014100525A2 (en) 2012-12-21 2014-06-26 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
US20150361447A1 (en) 2013-01-25 2015-12-17 Pioneer Hi-Breed International, Inc. Maize event dp-032218-9 and methods for detection thereof
WO2014164014A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Genes for improving nutrient uptake and abiotic stress tolerance in plants
US20160017360A1 (en) 2013-03-13 2016-01-21 Pioneer Hi-Bred International, Inc. Functional expression of bacterial major facilitator superfamily mfs gene in maize to improve agronomic traits and grain yield
US9713332B2 (en) 2013-03-13 2017-07-25 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in Brassica
WO2014164074A1 (en) 2013-03-13 2014-10-09 Pioneer Hi-Bred International, Inc. Enhanced nitrate uptake and nitrate translocation by over-expressing maize functional low-affinity nitrate transporters in transgenic maize
UA121847C2 (uk) 2013-03-14 2020-08-10 Піонір Хай-Бред Інтернешнл Інк. Полінуклеотид, що кодує елемент сайленсингу з інсектицидною активністю проти шкідника рослин із ряду coleoptera, та спосіб його застосування
US20160024513A1 (en) 2013-03-14 2016-01-28 Pioneer Hi-Bred International Inc. Maize stress related transcription factor 18 and uses thereof
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
BR112015023272A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int célula vegetal, planta, explante vegetal, semente transgênica, método para produzir uma célula vegetal tendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade de dicamba descarboxilase, método para controlar plantas daninhas em um campo contendo uma cultura e método para controlar plantas daninhas em um campo contendo uma cultura
BR112015023700A2 (pt) 2013-03-15 2017-07-18 Pioneer Hi Bred Int método de aprimoramento da tolerância ao estresse abiótico em uma planta de cultura agrícola , planta de milho transgênica, célula vegetal, semente, método de regulação negativa de um gene de acc oxidase endógeno em uma planta de milho.
CA2903297A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Modulation of acc deaminase expression
BR122022020536B1 (pt) 2013-08-08 2023-11-21 Pioneer Hi-Bred International, Inc. Molécula de ácido nucleico isolada, construto de dna, célula hospedeira bacteriana, polipeptídeo isolado, composição, método para controlar uma população, método para matar uma praga, método para produzir um polipeptídeo, método para produzir uma planta ou célula vegetal, método para proteger uma planta
BR112016008489A2 (pt) 2013-10-18 2017-10-03 Pioneer Hi Bred Int Sequências de glifosato-n-acetiltransferase (glyat) e métodos de uso
CA2961733A1 (en) 2014-09-17 2016-03-24 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN107074917B (zh) 2014-10-16 2022-05-24 先锋国际良种公司 具有改进的活性谱的杀昆虫多肽及其用途
RU2750459C2 (ru) 2014-10-16 2021-06-28 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные полипептиды с широким спектром активности и их применения
CN107771181A (zh) 2015-06-16 2018-03-06 先锋国际良种公司 用以防治昆虫有害生物的组合物和方法
CN108699117B (zh) 2016-04-14 2023-06-23 先锋国际良种公司 具有改善的活性谱的杀昆虫多肽及其用途
EP3445861B1 (de) 2016-04-19 2021-12-08 Pioneer Hi-Bred International, Inc. Insektizide kombinationen von polypeptiden mit verbessertem wirkungsspektrum und verwendungen davon
WO2017218207A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US11140902B2 (en) 2016-09-27 2021-10-12 University Of Florida Research Foundation, Inc. Insect toxin delivery mediated by a densovirus coat protein
WO2018140214A1 (en) 2017-01-24 2018-08-02 Pioneer Hi-Bred International, Inc. Nematicidal protein from pseudomonas
CN110914438A (zh) 2017-05-26 2020-03-24 先锋国际良种公司 具有改善的活性谱的杀昆虫多肽及其用途
CN111630061A (zh) 2017-12-19 2020-09-04 先锋国际良种公司 杀昆虫多肽及其用途
US11905518B2 (en) 2018-02-12 2024-02-20 Curators Of The University Of Missouri Small auxin upregulated (SAUR) gene for the improvement of root system architecture, waterlogging tolerance, drought resistance and yield in plants and methods of uses
BR112023002602A2 (pt) 2020-08-10 2023-04-04 Du Pont Composições e métodos para aumentar a resistência a helmintosporiose no milho
EP4251755A2 (de) 2020-11-24 2023-10-04 AgBiome, Inc. Pestizidgene und verfahren zur verwendung
BR112023023044A2 (pt) 2021-05-06 2024-01-23 Agbiome Inc Genes pesticidas e métodos de uso
WO2023107943A1 (en) 2021-12-07 2023-06-15 AgBiome, Inc. Pesticidal genes and methods of use
WO2024044596A1 (en) 2022-08-23 2024-02-29 AgBiome, Inc. Pesticidal genes and methods of use
WO2024129674A1 (en) 2022-12-13 2024-06-20 AgBiome, Inc. Pesticidal genes and methods of use

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2005252C (en) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
JP3315439B2 (ja) * 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的ｃＤＮＡの分離法及びライブラリー作製法
JP3337748B2 (ja) * 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法
FR2733762B1 (fr) * 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
JP3346976B2 (ja) * 1996-03-18 2002-11-18 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
US5891637A (en) * 1996-10-15 1999-04-06 Genentech, Inc. Construction of full length cDNA libraries

Also Published As

Publication number Publication date
JP3441899B2 (ja) 2003-09-02
CA2270414A1 (en) 1998-05-14
DE69731234D1 (de) 2004-11-18
WO1998020122A1 (fr) 1998-05-14
CA2270414C (en) 2007-12-04
DE69738603T2 (de) 2009-04-30
EP0990702B1 (de) 2004-10-13
EP0990702A1 (de) 2000-04-05
EP1469069B1 (de) 2008-03-26
DE69731234T2 (de) 2005-10-20
JPH10127291A (ja) 1998-05-19
EP1469069A1 (de) 2004-10-20
EP0990702A4 (de) 2001-05-30
US6143528A (en) 2000-11-07

Similar Documents

Publication Publication Date Title
DE69738603D1 (de) Verfahren zur Herstellung von Banken vollständiger cDNAs
DE69628712D1 (de) Verfahren zur Herstellung von Dihalogenazolopyrimidinen
DE69720379D1 (de) Verfahren zur Herstellung von Erythritol
DE59607656D1 (de) Verfahren zur Herstellung von Alkylhydrogenchlorsilanen
DE69702550D1 (de) Verfahren zur Herstellung von peroxiperfluoropolyxyalkylene
DE59703126D1 (de) Verfahren zur Herstellung von hochverzweigten Polyisoolefinen
DE69734526D1 (de) Verfahren zur herstellung von thiazolederivaten
DE59702177D1 (de) Verfahren zur Herstellung von Hydroxymethylcyclopropan
DE59610221D1 (de) Verfahren zur Herstellung von 2-Acetyl-gamma-butyrolacton
DE69703764D1 (de) Verfahren zur Herstellung von N-Acetonylbenzamide
DE69806876D1 (de) Verfahren zur Herstellung von 2-Chlorprop-1-en
DE69705291D1 (de) Verfahren zur Herstellung von Distyrol-biphenylverbindungen
DE59607351D1 (de) Verfahren zur Herstellung von Carbazaten
DE69600385D1 (de) Verfahren zur Herstellung von Aminonitropyridinen
DE69703599D1 (de) Verfahren zur Herstellung von 2-H-Heptafluorpropan
DE69737685D1 (de) Verfahren zur herstellung von hexanitrohexaazawurtzitaneen
DE69513008D1 (de) Verfahren zur Herstellung von Hydroxyalkoxybenzophenonen
DE69607541D1 (de) Verfahren zur Herstellung von p-Bromfluorobenzol
DE59605105D1 (de) Verfahren zur Herstellung von substituierten Aminocarbonyltriazolinonen
DE59802385D1 (de) Verfahren zur Herstellung von N-Alkylcarbazolen
DE59605227D1 (de) Verfahren zur Herstellung von ortho-Nitro-benzonitrilen
DE69705810D1 (de) Verfahren zur Herstellung von 2-Hydroxy-4-Alkyloxy-Benzophenon
DE59704518D1 (de) Verfahren zur Herstellung von Isocamphyl-cyclohexanolen
DE69700508D1 (de) Verfahren zur Herstellung von Organodisilanen
DE59705748D1 (de) Verfahren zur Herstellung von Cyclopentanolen

Legal Events

Date Code Title Description
8364 No opposition during term of opposition