DE69738528D1 - Gemischte g-proteinzusammensetzung und ihre verwendung - Google Patents

Gemischte g-proteinzusammensetzung und ihre verwendung

Info

Publication number
DE69738528D1
DE69738528D1 DE69738528T DE69738528T DE69738528D1 DE 69738528 D1 DE69738528 D1 DE 69738528D1 DE 69738528 T DE69738528 T DE 69738528T DE 69738528 T DE69738528 T DE 69738528T DE 69738528 D1 DE69738528 D1 DE 69738528D1
Authority
DE
Germany
Prior art keywords
mixed
protein composition
activation
compositions
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69738528T
Other languages
English (en)
Inventor
Paul A Negulescu
Stefan Offermanns
Melvin Simon
Charles Zuker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of DE69738528D1 publication Critical patent/DE69738528D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69738528T 1996-06-21 1997-06-20 Gemischte g-proteinzusammensetzung und ihre verwendung Expired - Lifetime DE69738528D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2023496P 1996-06-21 1996-06-21
PCT/US1997/010642 WO1997048820A1 (en) 1996-06-21 1997-06-20 Promiscuous g-protein compositions and their use

Publications (1)

Publication Number Publication Date
DE69738528D1 true DE69738528D1 (de) 2008-04-03

Family

ID=21797473

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69738528T Expired - Lifetime DE69738528D1 (de) 1996-06-21 1997-06-20 Gemischte g-proteinzusammensetzung und ihre verwendung

Country Status (7)

Country Link
US (2) US6004808A (de)
EP (1) EP1012324B1 (de)
AT (1) ATE386814T1 (de)
AU (1) AU3572897A (de)
CA (1) CA2281898C (de)
DE (1) DE69738528D1 (de)
WO (1) WO1997048820A1 (de)

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US6623922B1 (en) 1997-02-14 2003-09-23 Deltagen Proteomics Methods for identifying, characterizing, and evolving cell-type specific CIS regulatory elements
US5985584A (en) * 1997-02-19 1999-11-16 American Cyanamid Company Method to identify plant proteins that function in G protein coupled systems and compositions therefor
US7853411B2 (en) * 1997-02-27 2010-12-14 Cellomics, Inc. System for cell-based screening
US6759206B1 (en) 1997-02-27 2004-07-06 Cellomics, Inc. System for cell-based screening
US7087388B1 (en) * 1997-07-15 2006-08-08 Aurora Biosciences Corporation Compositions and methods for identifying modulators of transducisomes, a new class of therapeutic targets
US7262280B1 (en) 1998-04-03 2007-08-28 Nps Pharmaceuticals, Inc. G-protein fusion receptors and constructs encoding same
WO1999051641A1 (en) * 1998-04-03 1999-10-14 Nps Pharmaceuticals, Inc. G-protein fusion receptors and chimeric gabab receptors
US6692696B1 (en) * 1998-06-18 2004-02-17 ARETé ASSOCIATES Biosensor
DE19860833C1 (de) * 1998-12-30 2000-09-07 Albrecht E Sippel Methode zur zellulären High-Throughput(Hochdurchsatz)-Detektion von Rezeptor-Liganden-Interaktionen
US20020031802A1 (en) * 2000-05-12 2002-03-14 Yi Hu Novel seven transmembrane proteins and polynucleotides encoding the same
WO2000045179A2 (en) * 1999-01-27 2000-08-03 The Regents Of The University Of California Screening for modulators of g-protein subunit beta 3
US6929925B1 (en) 1999-01-27 2005-08-16 The Regents Of The University Of California Assays for sensory modulators using a sensory cell specific G-protein beta subunit
US7067277B1 (en) 1999-12-23 2006-06-27 H. Lundbeck A/S Chimeric G proteins and uses thereof
EP1143017A3 (de) * 2000-04-03 2004-01-28 Warner-Lambert Company LLC Methoden und Zusammensetzungen zum Screenen von icrac Modulatoren
EP1143013A1 (de) 2000-04-03 2001-10-10 Warner-Lambert Company Verfahren und Zusammenzetzungen zur Screening von ICRAC-Modulatoren
US7264926B2 (en) * 2000-04-18 2007-09-04 Millennium Pharmaceuticals, Inc. Nucleoside Phosphatase
JP2004500843A (ja) * 2000-04-24 2004-01-15 ワイエス トランスジェニック動物
AU2001259156A1 (en) * 2000-04-27 2001-11-07 Lexicon Genetics Incorporated Novel membrane proteins and polynucleotides encoding the same
DE10033353A1 (de) * 2000-07-08 2002-01-24 Aventis Pharma Gmbh Breit einsetzbares Verfahren zur Identifizierung von Modulatoren von G-Protein gekoppelten Rezeptoren
US6942988B2 (en) * 2000-09-22 2005-09-13 The Johns Hopkins University School Of Medicine Method of use of sonic hedgehog protein as a ligand for patched
WO2002036622A2 (en) * 2000-10-30 2002-05-10 Senomyx, Inc. GαqPROETIN VARIANTS AND THEIR USE IN THE ANALYSIS AND DISCOVERY OF AGONISTS AND ANTAGONISTS OF CHEMOSENSORY RECEPTORS
CA2438212A1 (en) * 2001-02-08 2002-09-06 Temple University - Of The Commonwealth System Of Higher Education Biosensor for detecting chemical agents
US20100248268A1 (en) * 2001-03-27 2010-09-30 Woods Daniel F Methods to utilize invertebrate chemosensory proteins for industrial and commercial uses
US20070003980A1 (en) * 2001-03-27 2007-01-04 Woods Daniel F Efficient methods to isolate effectors of proteins involved in olfactory or chemosensory pathways and efficient methods to use these effectors to alter organism olfaction, chemosensation, or behavior
DE60232141D1 (de) * 2001-03-27 2009-06-10 Inscent Inc Effiziente verfahren zur isolierung funktioneller g-protein-gekoppelter rezeptoren und identifizierung aktiver effektoren und effiziente verfahren zur isolierung an der geruchswahrnehmung beteiligter proteine sowie effiziente verfahren zur isolierung und identifizierung aktiver effektoren
WO2002086079A2 (en) * 2001-04-20 2002-10-31 Mount Sinai School Of Medicine T1r3 a novel taste receptor
US7803982B2 (en) 2001-04-20 2010-09-28 The Mount Sinai School Of Medicine Of New York University T1R3 transgenic animals, cells and related methods
PL370139A1 (en) * 2001-06-07 2005-05-16 Wyeth A g-protein coupled receptor and uses therefor
EP2003451B1 (de) 2001-06-26 2012-05-30 Senomyx, Inc. Heterooligomere t1r1-t1r3 Umamigeschmacksrezeptoren und diese exprimierende Zellinien sowie Verwendung davon zur Identifizierung von Umamigeschmacksverbindungen
US7238798B2 (en) 2001-08-14 2007-07-03 Sentisearch, Inc. Nucleic acids and proteins of insect Or83b odorant receptor genes and uses thereof
US7208290B2 (en) * 2001-12-14 2007-04-24 Senomyx, Inc. Methods of co-expressing umami taste receptors and chimeric Gα15 variants
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
SI1495330T1 (sl) * 2002-04-12 2009-06-30 Dowd Brian F O Postopki identificiranja spojin, ki medsebojno vplivajo na transmembranske proteine
JP2006515157A (ja) * 2002-07-29 2006-05-25 セノミックス、インコーポレイテッド 新規な苦味受容体ｔ２ｒ７６の同定
US20060019346A1 (en) * 2002-07-29 2006-01-26 Senomyx, Inc. Identification of a novel bitter taste receptor T2R76 that specifically responds to brucine and prop bitter ligands
DE10256947A1 (de) * 2002-12-05 2004-06-24 Grünenthal GmbH Verfahren zur Messung der Aktivität G(alpha)i- oder G(alpha)o-gekoppelter Rezeptoren mittels Ca2+-Influx in Zellen
WO2005010484A2 (en) * 2003-07-11 2005-02-03 Merck & Co., Inc. Methods for identifying cell surface receptor protein modulators
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
EP2418281B1 (de) 2003-10-24 2016-06-01 Gencia Corporation Verfahren und Zusammensetzungen zur Abgabe von Polynukleotiden
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
WO2005042574A1 (en) * 2003-11-03 2005-05-12 Acadia Pharmaceuticals Inc. High troughput functional assay for g-protein coupled receptors using a rap-ras chimeric protein
GB0421693D0 (en) 2004-09-30 2004-11-03 Amersham Biosciences Uk Ltd Method for measuring binding of a test compound to a G-protein coupled receptor
US20070218456A1 (en) * 2006-02-08 2007-09-20 Invitrogen Corporation Cellular assays for signaling receptors
US7998414B2 (en) * 2007-02-28 2011-08-16 Corning Incorporated System for high throughput GPCR functional assay
ES2727516T3 (es) * 2013-08-20 2019-10-16 Meiji Seika Pharma Co Ltd Métodos para evaluar y cribar agonistas del receptor de S1P1
JP6573972B2 (ja) * 2014-10-02 2019-09-11 パウル・シェラー・インスティトゥート 少なくとも１つの変異アミノ酸残基を有するヒトＧタンパク質αサブユニットＧαｉ１
EP3684797A4 (de) * 2017-09-19 2021-07-07 Cannametrix, LLC Zellbasierter test zur quantifizierung der potenz und wirksamkeit von cannabinoiden und/oder terpenoiden und verwendungsverfahren dafür
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Also Published As

Publication number Publication date
US6004808A (en) 1999-12-21
US20060008855A1 (en) 2006-01-12
CA2281898C (en) 2007-03-20
EP1012324A4 (de) 2003-08-13
AU3572897A (en) 1998-01-07
CA2281898A1 (en) 1997-12-24
EP1012324B1 (de) 2008-02-20
ATE386814T1 (de) 2008-03-15
EP1012324A1 (de) 2000-06-28
WO1997048820A1 (en) 1997-12-24

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