DE69734779T2 - Erhöhte ausbeuten an einem kristallisierten protein unter verwenden eines festen adsorptionsmaterials - Google Patents

Erhöhte ausbeuten an einem kristallisierten protein unter verwenden eines festen adsorptionsmaterials Download PDF

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Publication number
DE69734779T2
DE69734779T2 DE69734779T DE69734779T DE69734779T2 DE 69734779 T2 DE69734779 T2 DE 69734779T2 DE 69734779 T DE69734779 T DE 69734779T DE 69734779 T DE69734779 T DE 69734779T DE 69734779 T2 DE69734779 T2 DE 69734779T2
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DE
Germany
Prior art keywords
protein
crystallization
adsorption material
lipase
solid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69734779T
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German (de)
English (en)
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DE69734779D1 (de
Inventor
Stig Nielsson
Murmann Niels MADSEN
Curran Simpson
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Novozymes AS
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Novozymes AS
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Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of DE69734779D1 publication Critical patent/DE69734779D1/de
Publication of DE69734779T2 publication Critical patent/DE69734779T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/30Extraction; Separation; Purification by precipitation
    • C07K1/306Extraction; Separation; Purification by precipitation by crystallization
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Silicates, Zeolites, And Molecular Sieves (AREA)
DE69734779T 1996-03-14 1997-03-03 Erhöhte ausbeuten an einem kristallisierten protein unter verwenden eines festen adsorptionsmaterials Expired - Lifetime DE69734779T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK29596 1996-03-14
DK29596 1996-03-14
PCT/DK1997/000095 WO1997033905A1 (en) 1996-03-14 1997-03-03 Increased yields of a crystallized protein by using a solid adsorption material

Publications (2)

Publication Number Publication Date
DE69734779D1 DE69734779D1 (de) 2006-01-05
DE69734779T2 true DE69734779T2 (de) 2006-07-27

Family

ID=8091944

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69734779T Expired - Lifetime DE69734779T2 (de) 1996-03-14 1997-03-03 Erhöhte ausbeuten an einem kristallisierten protein unter verwenden eines festen adsorptionsmaterials

Country Status (9)

Country Link
EP (1) EP0942921B1 (enExample)
JP (1) JP2000506176A (enExample)
CN (1) CN1216898C (enExample)
AT (1) ATE311401T1 (enExample)
AU (1) AU2151697A (enExample)
DE (1) DE69734779T2 (enExample)
DK (1) DK0942921T3 (enExample)
IN (1) IN190885B (enExample)
WO (1) WO1997033905A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332600B2 (en) 2001-11-16 2008-02-19 Ranbaxy Laboratories Limited Process for the preparation of crystalline imipenem
CN102286067B (zh) * 2011-08-18 2014-04-02 西北工业大学 同时提高蛋白质结晶成功率及晶体质量的方法
CA3005292A1 (en) 2015-12-09 2017-06-15 Basf Se Method of purifying a protein from fermentation solids under desorbing conditions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB729208A (en) * 1951-06-15 1955-05-04 Pfizer & Co C Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof
US3966702A (en) * 1973-07-16 1976-06-29 Ralston Purina Company Process for the production of oilseed isolates
DK83093D0 (da) * 1993-07-09 1993-07-09 Novo Nordisk As Fremgangsmaade

Also Published As

Publication number Publication date
WO1997033905A1 (en) 1997-09-18
ATE311401T1 (de) 2005-12-15
DK0942921T3 (da) 2006-04-10
EP0942921B1 (en) 2005-11-30
DE69734779D1 (de) 2006-01-05
CN1216898C (zh) 2005-08-31
AU2151697A (en) 1997-10-01
IN190885B (enExample) 2003-08-30
CN1213376A (zh) 1999-04-07
EP0942921A1 (en) 1999-09-22
JP2000506176A (ja) 2000-05-23

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