CN1216898C - 利用固体吸附材料以提高的产率结晶蛋白质的方法 - Google Patents

利用固体吸附材料以提高的产率结晶蛋白质的方法 Download PDF

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Publication number
CN1216898C
CN1216898C CN97192948.3A CN97192948A CN1216898C CN 1216898 C CN1216898 C CN 1216898C CN 97192948 A CN97192948 A CN 97192948A CN 1216898 C CN1216898 C CN 1216898C
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CN
China
Prior art keywords
crystallization
carbon
lipase
protein
concentrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN97192948.3A
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English (en)
Chinese (zh)
Other versions
CN1213376A (zh
Inventor
S·尼尔森
N·M·麦德森
C·辛普森
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Novozymes AS
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Novozymes AS
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Publication date
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Publication of CN1213376A publication Critical patent/CN1213376A/zh
Application granted granted Critical
Publication of CN1216898C publication Critical patent/CN1216898C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Silicates, Zeolites, And Molecular Sieves (AREA)
CN97192948.3A 1996-03-14 1997-03-03 利用固体吸附材料以提高的产率结晶蛋白质的方法 Expired - Lifetime CN1216898C (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK0295/1996 1996-03-14
DK0295/96 1996-03-14
DK29596 1996-03-14

Publications (2)

Publication Number Publication Date
CN1213376A CN1213376A (zh) 1999-04-07
CN1216898C true CN1216898C (zh) 2005-08-31

Family

ID=8091944

Family Applications (1)

Application Number Title Priority Date Filing Date
CN97192948.3A Expired - Lifetime CN1216898C (zh) 1996-03-14 1997-03-03 利用固体吸附材料以提高的产率结晶蛋白质的方法

Country Status (9)

Country Link
EP (1) EP0942921B1 (enExample)
JP (1) JP2000506176A (enExample)
CN (1) CN1216898C (enExample)
AT (1) ATE311401T1 (enExample)
AU (1) AU2151697A (enExample)
DE (1) DE69734779T2 (enExample)
DK (1) DK0942921T3 (enExample)
IN (1) IN190885B (enExample)
WO (1) WO1997033905A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332600B2 (en) 2001-11-16 2008-02-19 Ranbaxy Laboratories Limited Process for the preparation of crystalline imipenem
CN102286067B (zh) * 2011-08-18 2014-04-02 西北工业大学 同时提高蛋白质结晶成功率及晶体质量的方法
CA3005292A1 (en) 2015-12-09 2017-06-15 Basf Se Method of purifying a protein from fermentation solids under desorbing conditions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB729208A (en) * 1951-06-15 1955-05-04 Pfizer & Co C Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof
US3966702A (en) * 1973-07-16 1976-06-29 Ralston Purina Company Process for the production of oilseed isolates
DK83093D0 (da) * 1993-07-09 1993-07-09 Novo Nordisk As Fremgangsmaade

Also Published As

Publication number Publication date
WO1997033905A1 (en) 1997-09-18
ATE311401T1 (de) 2005-12-15
DE69734779T2 (de) 2006-07-27
DK0942921T3 (da) 2006-04-10
EP0942921B1 (en) 2005-11-30
DE69734779D1 (de) 2006-01-05
AU2151697A (en) 1997-10-01
IN190885B (enExample) 2003-08-30
CN1213376A (zh) 1999-04-07
EP0942921A1 (en) 1999-09-22
JP2000506176A (ja) 2000-05-23

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Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
CB02 Change of applicant information

Applicant after: Novo Jymes A/S

Applicant before: Novo Nordisk A/S

COR Change of bibliographic data

Free format text: CORRECT: APPLICANT; FROM: NOVO NORDISK A/S TO: NUOWOQIMEIZI CO.,LTD.

C14 Grant of patent or utility model
GR01 Patent grant
CX01 Expiry of patent term

Granted publication date: 20050831

CX01 Expiry of patent term