DE69734061D1 - Subtrahierende amplifizierung - Google Patents

Subtrahierende amplifizierung

Info

Publication number
DE69734061D1
DE69734061D1 DE69734061T DE69734061T DE69734061D1 DE 69734061 D1 DE69734061 D1 DE 69734061D1 DE 69734061 T DE69734061 T DE 69734061T DE 69734061 T DE69734061 T DE 69734061T DE 69734061 D1 DE69734061 D1 DE 69734061D1
Authority
DE
Germany
Prior art keywords
subtractive amplification
subtractive
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69734061T
Other languages
English (en)
Other versions
DE69734061T2 (de
Inventor
T Malek
R Sooknanan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alethia Biotherapeutics Inc
Original Assignee
Alethia Biotherapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alethia Biotherapeutics Inc filed Critical Alethia Biotherapeutics Inc
Application granted granted Critical
Publication of DE69734061D1 publication Critical patent/DE69734061D1/de
Publication of DE69734061T2 publication Critical patent/DE69734061T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69734061T 1996-04-29 1997-04-29 Subtrahierende amplifizierung Expired - Lifetime DE69734061T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/639,763 US5712127A (en) 1996-04-29 1996-04-29 Subtractive amplification
PCT/US1997/007253 WO1997041260A2 (en) 1996-04-29 1997-04-29 Subtractive amplification

Publications (2)

Publication Number Publication Date
DE69734061D1 true DE69734061D1 (de) 2005-09-29
DE69734061T2 DE69734061T2 (de) 2006-06-14

Family

ID=24565452

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69734061T Expired - Lifetime DE69734061T2 (de) 1996-04-29 1997-04-29 Subtrahierende amplifizierung

Country Status (6)

Country Link
US (2) US5712127A (de)
EP (1) EP0914470B1 (de)
AU (1) AU737527B2 (de)
CA (1) CA2252801A1 (de)
DE (1) DE69734061T2 (de)
WO (1) WO1997041260A2 (de)

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WO2000024939A1 (en) 1998-10-27 2000-05-04 Affymetrix, Inc. Complexity management and analysis of genomic dna
US6638717B2 (en) 1999-05-19 2003-10-28 Aventis Pharmaceuticals, Inc. Microarray-based subtractive hybridzation
US20020119448A1 (en) * 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
WO2001009387A1 (en) * 1999-07-29 2001-02-08 Northeastern University Isolation of novel hemapoietic genes by representational difference analysis
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6379932B1 (en) * 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
US6465219B1 (en) 2000-08-07 2002-10-15 Genemed Biotechnologies, Inc. Polynucleotide pools enriched in either high-abundance or low-abundance sequences
US6861219B2 (en) 2000-09-25 2005-03-01 Genexpress Informatics, Inc. Preferential display
US20050084889A1 (en) * 2000-09-25 2005-04-21 Genexpress Informatics, Inc. Preferential display
ATE475720T1 (de) * 2000-12-13 2010-08-15 Nugen Technologies Inc Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
DE60220025T2 (de) 2001-03-09 2008-01-17 Nugen Technologies, Inc., San Carlos Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
US6632611B2 (en) * 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US7297778B2 (en) * 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US20040006033A1 (en) * 2001-08-06 2004-01-08 Zhu York Yuan-Yuan Methods for identifying low-abundance polynucleotides and related compositions
WO2004001065A2 (en) * 2002-06-24 2003-12-31 Cornell Research Foundation, Inc. Exhaustive selection or rna aptamers against complex targets
WO2004092418A2 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
WO2005003370A2 (en) * 2003-06-06 2005-01-13 Gene Logic, Inc. Methods for enhancing gene expression analysis
US20050221310A1 (en) * 2003-06-06 2005-10-06 Gene Ogic, Inc. Methods for enhancing gene expression analysis
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
JP2005080531A (ja) * 2003-09-05 2005-03-31 Tosoh Corp 腸炎ビブリオ菌の耐熱性溶血毒類似溶血毒素遺伝子の検出試薬
EP1623996A1 (de) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek
JP2008522632A (ja) * 2004-12-13 2008-07-03 アレシア・バイオセラピューティクス・インコーポレーテッド 骨再構築のプロセスに関与するポリヌクレオチド及びポリペプチド配列
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8168181B2 (en) 2006-02-13 2012-05-01 Alethia Biotherapeutics, Inc. Methods of impairing osteoclast differentiation using antibodies that bind siglec-15
DK1994155T4 (da) 2006-02-13 2022-07-25 Daiichi Sankyo Co Ltd Polynukleotid- og polypeptidsekvenser involveret i fremgangsmåden med knogleremodellering
EP2010659B1 (de) 2006-04-14 2014-06-18 CellScript, Inc. Kits und Verfahren zur Erzeugung von RNA mit 5'-Cap-Struktur
EP2502628B1 (de) 2006-06-23 2016-12-14 Alethia Biotherapeutics Inc. An Krebs beteiligte Polynukleotid- und Polypeptidsequenzen
CA2707436C (en) 2007-06-29 2014-01-28 Epicentre Technologies Corporation Copy dna and sense rna
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
WO2010026933A1 (ja) * 2008-09-03 2010-03-11 タカラバイオ株式会社 Ｒｎａ検出用組成物
MX336869B (es) 2008-11-03 2016-02-04 Alethia Biotherapeutics Inc Anticuerpos que bloquean espicificamente la actividad biologica de un antigeno de tumor.
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
US8822663B2 (en) 2010-08-06 2014-09-02 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CA3162352A1 (en) 2010-10-01 2012-04-05 Modernatx, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
AU2012236099A1 (en) 2011-03-31 2013-10-03 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
KR101993259B1 (ko) 2011-03-31 2019-06-27 에이디씨 테라퓨틱스 에스에이 신장 결합 항원 １에 대한 항체 및 이의 항원 결합 단편
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
US9428535B2 (en) 2011-10-03 2016-08-30 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
EP2791160B1 (de) 2011-12-16 2022-03-02 ModernaTX, Inc. Modifizierte mrna zusammensetzungen
DK2802351T3 (da) 2012-01-09 2019-05-13 Adc Therapeutics Sa Midler til behandling af triple-negativ brystkræft
US9303079B2 (en) 2012-04-02 2016-04-05 Moderna Therapeutics, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
AU2013243946A1 (en) 2012-04-02 2014-10-30 Moderna Therapeutics, Inc. Modified polynucleotides for the production of membrane proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
WO2014012165A1 (en) 2012-07-19 2014-01-23 Alethia Biotherapeutics Inc. Anti-siglec-15 antibodies
LT2922554T (lt) 2012-11-26 2022-06-27 Modernatx, Inc. Terminaliai modifikuota rnr
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
US10023626B2 (en) 2013-09-30 2018-07-17 Modernatx, Inc. Polynucleotides encoding immune modulating polypeptides
CA2926218A1 (en) 2013-10-03 2015-04-09 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor

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US5503979A (en) * 1984-05-25 1996-04-02 The Trustees Of Columbia University In The City Of New York Method of using replicatable hybridzable recombinant RNA probes
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
JP2650159B2 (ja) * 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA2003500C (en) * 1988-11-21 2000-01-18 Erik Hornes Process for producing cdna
IE66597B1 (en) * 1989-05-10 1996-01-24 Akzo Nv Method for the synthesis of ribonucleic acid (RNA)
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5459055A (en) * 1991-12-27 1995-10-17 Epicentre Technologies Corporation Thermostable ribonuclease H isolated from Thermus flavus
US5436142A (en) * 1992-11-12 1995-07-25 Cold Spring Harbor Laboratory Methods for producing probes capable of distingushing variant genomic sequences
CA2167838C (en) * 1993-07-23 1999-11-23 Thomas B. Ryder Methods for enhancing nucleic acid amplification
JP3881009B2 (ja) 1994-07-15 2007-02-14 バイオメリオ・ベー・ベー 核酸増幅方法を改良するためのｒｎａポリメラーゼの使用

Also Published As

Publication number Publication date
US6498024B1 (en) 2002-12-24
EP0914470B1 (de) 2005-08-24
WO1997041260A2 (en) 1997-11-06
EP0914470A2 (de) 1999-05-12
AU737527B2 (en) 2001-08-23
DE69734061T2 (de) 2006-06-14
AU3116497A (en) 1997-11-19
CA2252801A1 (en) 1997-11-06
US5712127A (en) 1998-01-27
WO1997041260A3 (en) 1998-01-22

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: DEHMEL & BETTENHAUSEN PATENT- UND RECHTSANWAELTE,