DE69726868D1 - DEFORMYLIERUNG VON f-MET PEPTIDEN IN BAKTERIEN EXPRESSIONSSYSTEMEN - Google Patents
DEFORMYLIERUNG VON f-MET PEPTIDEN IN BAKTERIEN EXPRESSIONSSYSTEMENInfo
- Publication number
- DE69726868D1 DE69726868D1 DE69726868T DE69726868T DE69726868D1 DE 69726868 D1 DE69726868 D1 DE 69726868D1 DE 69726868 T DE69726868 T DE 69726868T DE 69726868 T DE69726868 T DE 69726868T DE 69726868 D1 DE69726868 D1 DE 69726868D1
- Authority
- DE
- Germany
- Prior art keywords
- deformylation
- expression systems
- bacteria expression
- met peptides
- disclosed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000006198 deformylation Effects 0.000 title abstract 2
- 238000006344 deformylation reaction Methods 0.000 title abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 2
- 241000894006 Bacteria Species 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 abstract 1
- 108010026809 Peptide deformylase Proteins 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000003292 diminished effect Effects 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5409—IL-5
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/57—IFN-gamma
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2268796P | 1996-07-19 | 1996-07-19 | |
| US22687P | 1996-07-19 | ||
| PCT/US1997/012458 WO1998003664A1 (en) | 1996-07-19 | 1997-07-17 | DEFORMYLATION OF f-MET PEPTIDES IN BACTERIAL EXPRESSION SYSTEMS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69726868D1 true DE69726868D1 (de) | 2004-01-29 |
| DE69726868T2 DE69726868T2 (de) | 2004-10-28 |
Family
ID=21810912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69726868T Expired - Lifetime DE69726868T2 (de) | 1996-07-19 | 1997-07-17 | DEFORMYLIERUNG VON f-MET PEPTIDEN IN BAKTERIEN EXPRESSIONSSYSTEMEN |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US5834243A (de) |
| EP (1) | EP0917580B1 (de) |
| JP (1) | JP2000515017A (de) |
| KR (1) | KR100484602B1 (de) |
| CN (1) | CN1230994A (de) |
| AT (1) | ATE256748T1 (de) |
| AU (1) | AU731039B2 (de) |
| BR (1) | BR9710501B1 (de) |
| CA (1) | CA2261016A1 (de) |
| DE (1) | DE69726868T2 (de) |
| WO (1) | WO1998003664A1 (de) |
| ZA (1) | ZA976353B (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1230994A (zh) * | 1996-07-19 | 1999-10-06 | 孟山都公司 | 细菌表达系统中甲酰－甲硫氨酸肽的去甲酰化 |
| US6284878B1 (en) * | 1996-08-16 | 2001-09-04 | Smithkline Beecham Corporation | def1 |
| US6586578B1 (en) * | 1997-05-21 | 2003-07-01 | Smithkline Beecham Corporation | Defl |
| US5962666A (en) * | 1997-09-16 | 1999-10-05 | Smithkline Beecham Corporation | Deformylase |
| GB9724627D0 (en) * | 1997-11-20 | 1998-01-21 | Genencor Int Bv | Gram positive microorganism formate pathway |
| WO2000037486A1 (en) * | 1998-12-22 | 2000-06-29 | Holland Sweetener Company V.O.F. | Synthesis and recovery of aspartame involving enzymatic deformylation step |
| US6617127B2 (en) | 1998-12-22 | 2003-09-09 | Holland Sweetener Company, V.O.F. | Synthesis and recovery of aspartame involving enzymatic deformylation step |
| EP1013663A1 (de) * | 1998-12-22 | 2000-06-28 | Holland Sweetener Company V.O.F. | Synthese und Gewinnung von Aspartam unter Anwendung eines enzymatischen Deformylierungsschrittes |
| US6797820B2 (en) * | 1999-12-17 | 2004-09-28 | Vicuron Pharmaceuticals Inc. | Succinate compounds, compositions and methods of use and preparation |
| GB2365433A (en) * | 2000-05-25 | 2002-02-20 | Smithkline Beecham Corp | Human peptide deformylase |
| WO2003099854A2 (en) | 2002-05-24 | 2003-12-04 | Nps Allelix Corp. | Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides |
| DE60334912D1 (de) * | 2002-05-24 | 2010-12-23 | Medtronic Inc | Polypeptid-spaltungsprozess |
| ME01366B (me) | 2003-11-21 | 2013-12-20 | Nps Allelix Corp | POSTUPAK ZA PROIZVODNJU PEPTIDA 2 NALIK NA GLUKAGON l NJIHOVIH ANALOGA |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60500043A (ja) * | 1982-12-10 | 1985-01-17 | ノルデイスク・インスリンラボラトリウム | 原核細胞又は真核細胞中で合成される融合蛋白質から成熟蛋白質を作る方法 |
| CN1230994A (zh) * | 1996-07-19 | 1999-10-06 | 孟山都公司 | 细菌表达系统中甲酰－甲硫氨酸肽的去甲酰化 |
-
1997
- 1997-07-17 CN CN97198027A patent/CN1230994A/zh active Pending
- 1997-07-17 EP EP97933523A patent/EP0917580B1/de not_active Expired - Lifetime
- 1997-07-17 ZA ZA9706353A patent/ZA976353B/xx unknown
- 1997-07-17 KR KR10-1999-7000391A patent/KR100484602B1/ko not_active IP Right Cessation
- 1997-07-17 BR BRPI9710501-5A patent/BR9710501B1/pt not_active IP Right Cessation
- 1997-07-17 AU AU36689/97A patent/AU731039B2/en not_active Ceased
- 1997-07-17 WO PCT/US1997/012458 patent/WO1998003664A1/en active IP Right Grant
- 1997-07-17 CA CA002261016A patent/CA2261016A1/en not_active Abandoned
- 1997-07-17 DE DE69726868T patent/DE69726868T2/de not_active Expired - Lifetime
- 1997-07-17 JP JP10507072A patent/JP2000515017A/ja active Pending
- 1997-07-17 AT AT97933523T patent/ATE256748T1/de not_active IP Right Cessation
- 1997-07-17 US US08/895,939 patent/US5834243A/en not_active Expired - Lifetime
-
1998
- 1998-11-09 US US09/188,820 patent/US6190902B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0917580B1 (de) | 2003-12-17 |
| CA2261016A1 (en) | 1998-01-29 |
| ATE256748T1 (de) | 2004-01-15 |
| CN1230994A (zh) | 1999-10-06 |
| DE69726868T2 (de) | 2004-10-28 |
| US6190902B1 (en) | 2001-02-20 |
| AU731039B2 (en) | 2001-03-22 |
| JP2000515017A (ja) | 2000-11-14 |
| BR9710501A (pt) | 1999-08-17 |
| KR100484602B1 (ko) | 2005-04-20 |
| ZA976353B (en) | 1998-02-03 |
| BR9710501B1 (pt) | 2009-12-01 |
| EP0917580A1 (de) | 1999-05-26 |
| WO1998003664A1 (en) | 1998-01-29 |
| KR20000067927A (ko) | 2000-11-25 |
| US5834243A (en) | 1998-11-10 |
| AU3668997A (en) | 1998-02-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69726868D1 (de) | DEFORMYLIERUNG VON f-MET PEPTIDEN IN BAKTERIEN EXPRESSIONSSYSTEMEN | |
| DE69841939D1 (de) | Direkte expression von peptiden ins kulturmedium | |
| ATE160587T1 (de) | Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung | |
| ATE189478T1 (de) | Für die reinigung bestimmte klonierung von peptiden | |
| WO1993023434A3 (en) | Bpi-immunoglobulin fusion proteins | |
| BR9609325A (pt) | Uso de um polipeptídeo ou proteína com sequência de aminoácidos processos para preparar um produto melhorado e para produzir polipeptídeos ou proteínas recombinantes organismo hospedeiro recombinante de tipo alimentício produto e polipeptídeo ou proteína de tipo alimentício recombinante substancialmente puro ou isolado | |
| DE69535147D1 (de) | Überexpression von säugetier- und virusproteinen | |
| ATE317904T1 (de) | Überexpression von phytase in hefesystemen | |
| DE69634819D1 (de) | Reguliertes autokrines wachstum von säugerzellen | |
| WO2000034474A3 (en) | Growth factor homolog zvegf3 | |
| CA2249180A1 (en) | Methods for recombinant microbial production of bpi-fusion proteins and bpi-derived peptides | |
| AU6435394A (en) | Fusion proteins between antigenic amino acid sequences and beta-2-microglobulin | |
| EP0875567A3 (de) | Myc-bindende Zinkfinger-Proteine, ihre Herstellung und ihre Verwendung | |
| AU2001231881A1 (en) | Oligomers of nonpeptide restricted mimetics of dipeptides or tripeptides and theuse thereof in the synthesis of synthetic proteins and polypeptides | |
| Rühlmann et al. | Complete amino acid sequence of protein B | |
| WO1999002708A8 (en) | Fusion proteins comprising sequences derived from bovine if1 atpase inhibitor protein | |
| DE60023933D1 (en) | Hybrid-toxine von bacillus thuringiensis | |
| Fauvel et al. | Aortic endothelial cells in culture secrete glycoproteins reacting with blood platelets | |
| Chestukhina et al. | Amino acid sequences from the N-terminal domain of Bacillus thuringiensis, subspecies alesti, δ-endotoxin: Hypervariable regions of Bacillus thuringiensis δ-endotoxms | |
| WO2001047958A3 (en) | Kunitz domain polypeptide zkun10 | |
| Edwards et al. | Pigeon muscle aldolase: Kinetic properties and primary structure close to the substrate binding lysine residue | |
| DE69030316D1 (de) | Schrittweiser c-terminaler abbau von peptiden und proteinen | |
| AU3818095A (en) | Separation of amino acids and peptides from protein hydrolysates | |
| CA2227789A1 (en) | Regulated autocrine growth of mammalian cells | |
| SE9404066D0 (sv) | Fusion proteins of immunopotentiating activity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |