DE69633238D1 - Methode zur erhöhung der enzymdiversität - Google Patents

Methode zur erhöhung der enzymdiversität

Info

Publication number
DE69633238D1
DE69633238D1 DE69633238T DE69633238T DE69633238D1 DE 69633238 D1 DE69633238 D1 DE 69633238D1 DE 69633238 T DE69633238 T DE 69633238T DE 69633238 T DE69633238 T DE 69633238T DE 69633238 D1 DE69633238 D1 DE 69633238D1
Authority
DE
Germany
Prior art keywords
sequence
enzyme
antibody
animal
variable region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69633238T
Other languages
English (en)
Other versions
DE69633238T2 (de
Inventor
Jacob N Wohlstadter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/476,135 external-priority patent/US5914256A/en
Priority claimed from US08/505,528 external-priority patent/US5919681A/en
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE69633238D1 publication Critical patent/DE69633238D1/de
Publication of DE69633238T2 publication Critical patent/DE69633238T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0002—Antibodies with enzymatic activity, e.g. abzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69633238T 1995-06-07 1996-06-06 Methode zur erhöhung der enzymdiversität Expired - Lifetime DE69633238T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US505528 1995-06-07
US476135 1995-06-07
US08/476,135 US5914256A (en) 1995-06-07 1995-06-07 Method for promoting enzyme diversity
US08/505,528 US5919681A (en) 1995-06-07 1995-06-07 Method for promoting enzyme diversity
PCT/US1996/009827 WO1996040960A1 (en) 1995-06-07 1996-06-06 A method for promoting enzyme diversity

Publications (2)

Publication Number Publication Date
DE69633238D1 true DE69633238D1 (de) 2004-09-30
DE69633238T2 DE69633238T2 (de) 2005-09-15

Family

ID=27045085

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69633238T Expired - Lifetime DE69633238T2 (de) 1995-06-07 1996-06-06 Methode zur erhöhung der enzymdiversität

Country Status (11)

Country Link
US (2) US5922584A (de)
EP (2) EP0839208B1 (de)
JP (1) JP4102435B2 (de)
AT (1) ATE274594T1 (de)
AU (1) AU719670B2 (de)
CA (1) CA2223716C (de)
DE (1) DE69633238T2 (de)
DK (1) DK0839208T3 (de)
ES (1) ES2227594T3 (de)
WO (1) WO1996040960A1 (de)
ZA (1) ZA964889B (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE431407T1 (de) * 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo Verfahren zur herstellung von dna
EP2825653A4 (de) 2012-03-14 2016-01-20 Innovative Targeting Solutions Inc Erzeugung einer gezielten sequenzdiversität in fusionsproteinen
CA2867231C (en) * 2012-03-14 2021-10-19 Innovative Targeting Solutions Inc. Generating targeted sequence diversity in proteins

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156965A (en) * 1983-11-29 1992-10-20 Igen, Inc. Catalytic antibodies
US5037750A (en) * 1984-11-27 1991-08-06 Igen, Inc. Method for producing antibodies which catalyze chemical reactions
US4963355A (en) * 1986-06-23 1990-10-16 Igen, Inc. Production of antibody catalysts
US4792446A (en) * 1986-06-23 1988-12-20 Igen, Inc. Production of antibody catalysts
GB8823869D0 (en) * 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5318897A (en) * 1989-04-25 1994-06-07 Igen, Inc. Monoclonal antibody and antibody components elicited to a polypeptide antigen ground state
WO1991010741A1 (en) * 1990-01-12 1991-07-25 Cell Genesys, Inc. Generation of xenogeneic antibodies
EP0546073B1 (de) * 1990-08-29 1997-09-10 GenPharm International, Inc. Produktion und Nützung nicht-menschliche transgentiere zur Produktion heterologe Antikörper
ATE280219T1 (de) * 1992-03-16 2004-11-15 Jacob Nathaniel Wohlstadter Mutagenese und selektionsmethoden in den selben wirtszellen
JPH08509612A (ja) * 1993-04-26 1996-10-15 ジェンファーム インターナショナル インコーポレイテッド 異種抗体を産生することができるトランスジェニック非ヒト動物
WO1994025586A1 (en) * 1993-04-30 1994-11-10 The Scripps Research Institute Transgenic animals having an engineered immune response
US5919681A (en) * 1995-06-07 1999-07-06 Wohlstadter Jacob N Method for promoting enzyme diversity
US5914256A (en) * 1995-06-07 1999-06-22 Wohlstadter Jacob N Method for promoting enzyme diversity

Also Published As

Publication number Publication date
CA2223716A1 (en) 1996-12-19
AU719670B2 (en) 2000-05-18
EP0839208B1 (de) 2004-08-25
WO1996040960A1 (en) 1996-12-19
EP0839208A4 (de) 2002-05-15
US6242236B1 (en) 2001-06-05
AU6167496A (en) 1996-12-30
JPH11513884A (ja) 1999-11-30
EP0839208A1 (de) 1998-05-06
DE69633238T2 (de) 2005-09-15
JP4102435B2 (ja) 2008-06-18
US5922584A (en) 1999-07-13
DK0839208T3 (da) 2004-12-13
ES2227594T3 (es) 2005-04-01
ATE274594T1 (de) 2004-09-15
EP1437414A2 (de) 2004-07-14
CA2223716C (en) 2012-03-13
ZA964889B (en) 1997-07-08
EP1437414A3 (de) 2005-01-05

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Legal Events

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