DE69624076D1 - Verfahren zur Klonierung und Herstellung einer Sca1-Restriktionsendonuklease in E. Coli - Google Patents

Verfahren zur Klonierung und Herstellung einer Sca1-Restriktionsendonuklease in E. Coli

Info

Publication number
DE69624076D1
DE69624076D1 DE69624076T DE69624076T DE69624076D1 DE 69624076 D1 DE69624076 D1 DE 69624076D1 DE 69624076 T DE69624076 T DE 69624076T DE 69624076 T DE69624076 T DE 69624076T DE 69624076 D1 DE69624076 D1 DE 69624076D1
Authority
DE
Germany
Prior art keywords
cloning
coli
producing
restriction endonuclease
sca1
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69624076T
Other languages
English (en)
Other versions
DE69624076T2 (de
Inventor
Shuang-Yong Xu
Jian-Ping Xiao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of DE69624076D1 publication Critical patent/DE69624076D1/de
Publication of DE69624076T2 publication Critical patent/DE69624076T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE69624076T 1995-12-08 1996-11-29 Verfahren zur Klonierung und Herstellung einer Sca1-Restriktionsendonuklease in E. Coli Expired - Lifetime DE69624076T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/569,806 US5721126A (en) 1995-12-08 1995-12-08 Method for cloning and producing the SCaI restriction endonuclease in E. coli

Publications (2)

Publication Number Publication Date
DE69624076D1 true DE69624076D1 (de) 2002-11-07
DE69624076T2 DE69624076T2 (de) 2003-04-30

Family

ID=24276936

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69624076T Expired - Lifetime DE69624076T2 (de) 1995-12-08 1996-11-29 Verfahren zur Klonierung und Herstellung einer Sca1-Restriktionsendonuklease in E. Coli

Country Status (4)

Country Link
US (1) US5721126A (de)
EP (1) EP0778346B1 (de)
JP (1) JP3889098B2 (de)
DE (1) DE69624076T2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532153A (en) * 1995-03-23 1996-07-02 New England Biolabs, Inc. Method for cloning and producing the SacI restriction endonuclease
US5945288A (en) * 1997-11-24 1999-08-31 New England Biolabs, Inc. Method for cloning and producing the PmeI restriction endonuclease
US5885818A (en) * 1998-05-14 1999-03-23 New England Biolabs, Inc. Method for cloning and producing ageI restriction endonuclease in E. coli
US6025179A (en) * 1998-08-31 2000-02-15 New England Biolabs, Inc. Method for cloning and producing the SnaBI restriction endonuclease and purification of the recombinant SnaBI restriction endonuclease
US6403354B1 (en) * 2001-01-19 2002-06-11 New England Biolabs, Inc. Method for cloning and expression of BstYI restriction endonuclease and BstYI methylase in E. coli and purification of BstYI and M.BstYI enzymes
WO2007097778A2 (en) 2005-08-04 2007-08-30 New England Biolabs, Inc. Novel restriction endonucleases, dna encoding these endonucleases and methods for identifying new endonucleases with the same or varied specificity
CN105483097B (zh) * 2007-07-12 2021-07-13 新英格兰生物实验室公司 高保真度限制性内切核酸酶

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
US5288696A (en) * 1990-09-07 1994-02-22 New England Biolabs, Inc. Method for producing and cloning SacII restriction endonuclease and methylase

Also Published As

Publication number Publication date
EP0778346A2 (de) 1997-06-11
DE69624076T2 (de) 2003-04-30
US5721126A (en) 1998-02-24
JPH09275989A (ja) 1997-10-28
JP3889098B2 (ja) 2007-03-07
EP0778346B1 (de) 2002-10-02
EP0778346A3 (de) 1998-06-10

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition