DE69731511D1 - Verfahren zur Klonierung und Herstellung einer SapI Restriktionsendonuklease in E. coli - Google Patents

Verfahren zur Klonierung und Herstellung einer SapI Restriktionsendonuklease in E. coli

Info

Publication number
DE69731511D1
DE69731511D1 DE69731511T DE69731511T DE69731511D1 DE 69731511 D1 DE69731511 D1 DE 69731511D1 DE 69731511 T DE69731511 T DE 69731511T DE 69731511 T DE69731511 T DE 69731511T DE 69731511 D1 DE69731511 D1 DE 69731511D1
Authority
DE
Germany
Prior art keywords
cloning
coli
producing
restriction endonuclease
sapi restriction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69731511T
Other languages
English (en)
Other versions
DE69731511T2 (de
Inventor
Shuang-Yong Xu
Jianping Xiao
Robert E Maunus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of DE69731511D1 publication Critical patent/DE69731511D1/de
Application granted granted Critical
Publication of DE69731511T2 publication Critical patent/DE69731511T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
DE69731511T 1996-07-11 1997-07-02 Verfahren zur Klonierung und Herstellung einer SapI Restriktionsendonuklease in E. coli Expired - Lifetime DE69731511T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/678,304 US5663067A (en) 1996-07-11 1996-07-11 Method for cloning and producing the SapI restriction endonuclease in E. coli
US678304 1996-07-11

Publications (2)

Publication Number Publication Date
DE69731511D1 true DE69731511D1 (de) 2004-12-16
DE69731511T2 DE69731511T2 (de) 2005-11-24

Family

ID=24722261

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69731511T Expired - Lifetime DE69731511T2 (de) 1996-07-11 1997-07-02 Verfahren zur Klonierung und Herstellung einer SapI Restriktionsendonuklease in E. coli

Country Status (4)

Country Link
US (1) US5663067A (de)
EP (1) EP0818537B1 (de)
JP (1) JP4172661B2 (de)
DE (1) DE69731511T2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025179A (en) * 1998-08-31 2000-02-15 New England Biolabs, Inc. Method for cloning and producing the SnaBI restriction endonuclease and purification of the recombinant SnaBI restriction endonuclease
US20040038283A1 (en) * 2001-04-30 2004-02-26 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
EP1847611B1 (de) * 2003-10-03 2009-09-09 Promega Corporation Rhamnose-induzierbares Expressionssystem
EP2548953A1 (de) 2005-08-04 2013-01-23 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
EP2390317B1 (de) * 2007-07-12 2014-06-25 New England Biolabs, Inc. Restriktionsendonukleasen mit hoher Fidelität
WO2018226893A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A high-throughput (htp) genomic engineering platform for improving saccharopolyspora spinosa

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200333A (en) * 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
US5498535A (en) * 1994-05-24 1996-03-12 New England Biolabs, Inc. Method for direct cloning of nuclease genes in E. coli
US5532153A (en) * 1995-03-23 1996-07-02 New England Biolabs, Inc. Method for cloning and producing the SacI restriction endonuclease

Also Published As

Publication number Publication date
JPH1057082A (ja) 1998-03-03
EP0818537B1 (de) 2004-11-10
EP0818537A2 (de) 1998-01-14
EP0818537A3 (de) 1999-08-04
US5663067A (en) 1997-09-02
DE69731511T2 (de) 2005-11-24
JP4172661B2 (ja) 2008-10-29

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Legal Events

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8364 No opposition during term of opposition