DE69617773D1 - Verfahren zur Messung von Oligonukleotidabbau - Google Patents

Verfahren zur Messung von Oligonukleotidabbau

Info

Publication number
DE69617773D1
DE69617773D1 DE69617773T DE69617773T DE69617773D1 DE 69617773 D1 DE69617773 D1 DE 69617773D1 DE 69617773 T DE69617773 T DE 69617773T DE 69617773 T DE69617773 T DE 69617773T DE 69617773 D1 DE69617773 D1 DE 69617773D1
Authority
DE
Germany
Prior art keywords
oligonucleotide degradation
measuring
measuring oligonucleotide
degradation
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69617773T
Other languages
English (en)
Other versions
DE69617773T2 (de
Inventor
Hisatoshi Uchiyama
Masaki Jibu
Kenichi Hirano
Kazunari Taira
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hamamatsu Photonics KK
Original Assignee
Hamamatsu Photonics KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hamamatsu Photonics KK filed Critical Hamamatsu Photonics KK
Application granted granted Critical
Publication of DE69617773D1 publication Critical patent/DE69617773D1/de
Publication of DE69617773T2 publication Critical patent/DE69617773T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69617773T 1995-03-10 1996-03-08 Verfahren zur Messung von Oligonukleotidabbau Expired - Fee Related DE69617773T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5092695 1995-03-10

Publications (2)

Publication Number Publication Date
DE69617773D1 true DE69617773D1 (de) 2002-01-24
DE69617773T2 DE69617773T2 (de) 2002-05-08

Family

ID=12872422

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69617773T Expired - Fee Related DE69617773T2 (de) 1995-03-10 1996-03-08 Verfahren zur Messung von Oligonukleotidabbau

Country Status (3)

Country Link
US (1) US5843658A (de)
EP (1) EP0731178B1 (de)
DE (1) DE69617773T2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5728528A (en) * 1995-09-20 1998-03-17 The Regents Of The University Of California Universal spacer/energy transfer dyes
US7825237B2 (en) * 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7388092B2 (en) * 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5853992A (en) * 1996-10-04 1998-12-29 The Regents Of The University Of California Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels
JP2001526052A (ja) * 1997-12-12 2001-12-18 インタージェン カンパニー Ｆｒｅｔ標識オリゴヌクレオチドを用いたアポトーシスを検出する方法
DE19811618C1 (de) * 1998-03-17 2000-08-31 Andreas Jenne Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten
US6358684B1 (en) 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
CA2382063A1 (en) * 1999-09-17 2001-03-22 Shiv Kumar Charge-modified nucleic acid terminators
US7125660B2 (en) * 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
CA2425663C (en) 2000-10-11 2009-12-29 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
US20030119079A1 (en) * 2001-09-19 2003-06-26 The Regents Of The University Of Michigan Detection and treatment of cancers of the breast
FR2836926A1 (fr) * 2002-06-14 2003-09-12 Commissariat Energie Atomique Substrat oligonucleotidique et procede permettant d'analyser la presence d'activites de reparation de lesions de l'adn
US20040209353A1 (en) * 2002-12-12 2004-10-21 Chiron Corporation Biological sample storage device and method for biological sample contamination testing
CA2520664A1 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. Targeting enzymes of the trna splicing pathway for identification of anti-fungal and/or anti-proliferative molecules
JP4634371B2 (ja) 2003-03-27 2011-02-16 ピーティーシー セラピューティクス，インコーポレーテッド ｔＲＮＡスプライシングエンドヌクレアーゼに標的化する化合物の同定方法、および該化合物の抗増殖剤としての使用
FR2888938B1 (fr) * 2005-07-21 2013-09-27 Commissariat Energie Atomique Substrats fluorescents saccharidiques, leur procede de preparation et leurs utilisations
PL389135A1 (pl) * 2009-09-28 2011-04-11 Michał Lower Sposób i sonda DNA do otrzymywania endonukleaz restrukcyjnych, zwłaszcza o pożądanej specyficzności sekwencyjnej
US9404160B2 (en) 2009-12-22 2016-08-02 Becton, Dickinson And Company Methods for the detection of microorganisms

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5037615A (en) * 1987-10-30 1991-08-06 Cordis Corporation Tethered pair fluorescence energy transfer indicators, chemical sensors, and method of making such sensors
EP0428000A1 (de) * 1989-11-03 1991-05-22 Abbott Laboratories Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
GB9310978D0 (en) * 1993-05-27 1993-07-14 Zeneca Ltd Compounds

Also Published As

Publication number Publication date
EP0731178A2 (de) 1996-09-11
EP0731178B1 (de) 2001-12-12
DE69617773T2 (de) 2002-05-08
EP0731178A3 (de) 1998-04-22
US5843658A (en) 1998-12-01

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee