DE69605712T2 - Anwendung von isoelektrischen Punkt Markern bei der isoelektrischen Fokussierung mit Fluoreszenzdetektierung - Google Patents
Anwendung von isoelektrischen Punkt Markern bei der isoelektrischen Fokussierung mit FluoreszenzdetektierungInfo
- Publication number
- DE69605712T2 DE69605712T2 DE69605712T DE69605712T DE69605712T2 DE 69605712 T2 DE69605712 T2 DE 69605712T2 DE 69605712 T DE69605712 T DE 69605712T DE 69605712 T DE69605712 T DE 69605712T DE 69605712 T2 DE69605712 T2 DE 69605712T2
- Authority
- DE
- Germany
- Prior art keywords
- isoelectric
- application
- fluorescence detection
- point markers
- focusing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
- C07K1/28—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2410/00—Assays, e.g. immunoassays or enzyme assays, involving peptides of less than 20 animo acids
- G01N2410/02—Angiotensins; Related peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/28—Bound to a nonpeptide drug, nonpeptide label, nonpeptide carrier, or a nonpeptide resin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Electrochemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Optics & Photonics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7687395 | 1995-03-31 | ||
JP27119695 | 1995-10-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69605712D1 DE69605712D1 (de) | 2000-01-27 |
DE69605712T2 true DE69605712T2 (de) | 2000-04-27 |
Family
ID=26417993
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69605712T Expired - Fee Related DE69605712T2 (de) | 1995-03-31 | 1996-03-29 | Anwendung von isoelektrischen Punkt Markern bei der isoelektrischen Fokussierung mit Fluoreszenzdetektierung |
Country Status (3)
Country | Link |
---|---|
US (1) | US5866683A (de) |
EP (1) | EP0744614B1 (de) |
DE (1) | DE69605712T2 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19954934A1 (de) * | 1999-11-16 | 2001-05-17 | Otto S Wolfbeis | Verfahren zur Solubilisierung von optischen Markern |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU6020998A (en) | 1997-01-08 | 1998-08-03 | Life Technologies, Inc. | Methods for production of proteins |
WO2001009165A2 (en) * | 1999-07-29 | 2001-02-08 | The Johns Hopkins University | ACTIVATION OF PEPTIDE PRODRUGS BY hK2 |
KR100564085B1 (ko) * | 1999-11-30 | 2006-03-27 | 일렉트릭 파워 리서치 인스티튜트 | 캐필러리 전기영동 프로브 및 방법 |
WO2002013848A1 (en) * | 2000-08-11 | 2002-02-21 | Invitrogen Corporation | Highly homogeneous molecular markers for electrophoresis |
US20040014082A1 (en) * | 2000-08-11 | 2004-01-22 | Invitrogen Corporation | Highly homogeneous molecular markers for electrophoresis |
AU2003211133A1 (en) * | 2002-02-20 | 2003-09-09 | Invitrogen Corporation | Highly homogeneous molecular markers for electrophoresis |
WO2005030981A2 (en) * | 2003-09-25 | 2005-04-07 | Invitrogen Corporation | Homogeneous populations of molecules |
US7407816B2 (en) * | 2004-05-07 | 2008-08-05 | Gentius, Inc | Isoelectric particles and uses thereof |
US20060292649A1 (en) * | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for reference lab diagnostics |
US7846676B2 (en) * | 2004-07-19 | 2010-12-07 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
WO2006014680A1 (en) | 2004-07-19 | 2006-02-09 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
US20060292558A1 (en) * | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for protein assay diagnostics |
US7935479B2 (en) * | 2004-07-19 | 2011-05-03 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
JP2008536128A (ja) * | 2005-04-09 | 2008-09-04 | セル バイオサイエンシズ,インコーポレイテッド | 自動化微小体積アッセイシステム |
US20060286378A1 (en) * | 2005-05-23 | 2006-12-21 | Shivkumar Chiruvolu | Nanostructured composite particles and corresponding processes |
WO2007035864A2 (en) * | 2005-09-20 | 2007-03-29 | Cell Biosciences, Inc. | Electrophoresis standards, methods and kits |
EP2069386A4 (de) | 2006-07-21 | 2009-10-28 | Life Technologies Corp | Proteinmolekülgewichtsstandards mit hoher trennschärfe |
GB0714202D0 (en) * | 2007-07-20 | 2007-08-29 | Ge Healthcare Bio Sciences Ab | Isoelectric point markers |
US20090023156A1 (en) * | 2007-07-20 | 2009-01-22 | Voss Karl O | Methods and reagents for quantifying analytes |
CN101556261B (zh) * | 2009-05-25 | 2012-01-11 | 北京理工大学 | 微管内等电聚焦和剪断吹出培养测定微生物等电点的方法 |
CA2817671A1 (en) * | 2009-11-17 | 2011-05-26 | Universite De Montreal | Heteropeptides useful for reducing nonspecific adsorption |
US11782023B2 (en) | 2018-12-19 | 2023-10-10 | Regeneron Pharmaceuticals, Inc. | Ce-western applications for antibody development |
AU2020279380A1 (en) | 2019-05-21 | 2021-12-09 | Regeneron Pharmaceuticals, Inc. | Methods for identifying and quantitating host cell protein |
CN115010789B (zh) * | 2022-06-20 | 2023-10-20 | 湖州申科生物技术股份有限公司 | 用于蛋白质双向电泳中等电聚焦质控的荧光多肽及其制备方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5439829A (en) * | 1991-01-30 | 1995-08-08 | Eli Lilly And Company | Immobilization of biologically active molecules by changing the Oxidation state of a chelated transition metal ion |
US5245551A (en) * | 1991-04-10 | 1993-09-14 | University Of Utah Research Foundation | Method of determining extinction coefficient of fluorescent dye and protein concentration of dye-protein conjugate |
FR2687680A1 (fr) | 1992-02-20 | 1993-08-27 | Centre Nat Rech Scient | Procede de marquage de proteines et de peptides par acylation de leur fonction alpha aminee par un reactif comportant une fonction carboxylique activee. |
CA2086453A1 (en) * | 1992-12-30 | 1994-07-01 | Mcgill University | Marker for neurotensin receptor |
US5348633A (en) * | 1993-01-22 | 1994-09-20 | Northeastern University | Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis |
SE501192C2 (sv) | 1993-04-29 | 1994-12-05 | Flygt Ab Itt | Sätt och anordning att styra en ventil för att åstadkomma återcirkulation i avloppsvattenpumpstationer |
JPH07271196A (ja) | 1994-03-28 | 1995-10-20 | Fuji Photo Film Co Ltd | 平版作製装置 |
-
1996
- 1996-03-29 US US08/623,833 patent/US5866683A/en not_active Expired - Fee Related
- 1996-03-29 EP EP96105113A patent/EP0744614B1/de not_active Expired - Lifetime
- 1996-03-29 DE DE69605712T patent/DE69605712T2/de not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19954934A1 (de) * | 1999-11-16 | 2001-05-17 | Otto S Wolfbeis | Verfahren zur Solubilisierung von optischen Markern |
Also Published As
Publication number | Publication date |
---|---|
EP0744614A3 (de) | 1998-06-10 |
EP0744614A2 (de) | 1996-11-27 |
US5866683A (en) | 1999-02-02 |
EP0744614B1 (de) | 1999-12-22 |
DE69605712D1 (de) | 2000-01-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |