DE69532646D1 - Verfahren zur herstellung extrazellulärer proteine in bakterien - Google Patents

Verfahren zur herstellung extrazellulärer proteine in bakterien

Info

Publication number
DE69532646D1
DE69532646D1 DE69532646T DE69532646T DE69532646D1 DE 69532646 D1 DE69532646 D1 DE 69532646D1 DE 69532646 T DE69532646 T DE 69532646T DE 69532646 T DE69532646 T DE 69532646T DE 69532646 D1 DE69532646 D1 DE 69532646D1
Authority
DE
Germany
Prior art keywords
protein
prepropeptide
sequence encoding
cell membrane
recombinant vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69532646T
Other languages
English (en)
Other versions
DE69532646T2 (de
Inventor
Woeldike Helle Fabricius
Sven Hastrup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of DE69532646D1 publication Critical patent/DE69532646D1/de
Publication of DE69532646T2 publication Critical patent/DE69532646T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/824—Achromobacter

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69532646T 1994-12-09 1995-12-08 Verfahren zur herstellung extrazellulärer proteine in bakterien Expired - Lifetime DE69532646T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK141194 1994-12-09
DK141194 1994-12-09
PCT/DK1995/000498 WO1996017943A1 (en) 1994-12-09 1995-12-08 A process of producing extracellular proteins in bacteria

Publications (2)

Publication Number Publication Date
DE69532646D1 true DE69532646D1 (de) 2004-04-08
DE69532646T2 DE69532646T2 (de) 2005-03-03

Family

ID=8104544

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69532646T Expired - Lifetime DE69532646T2 (de) 1994-12-09 1995-12-08 Verfahren zur herstellung extrazellulärer proteine in bakterien

Country Status (7)

Country Link
US (1) US6171823B1 (de)
EP (1) EP0797671B1 (de)
JP (1) JP3920331B2 (de)
AT (1) ATE260979T1 (de)
AU (1) AU4114596A (de)
DE (1) DE69532646T2 (de)
WO (1) WO1996017943A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0797671B1 (de) * 1994-12-09 2004-03-03 Novo Nordisk A/S Verfahren zur herstellung extrazellulärer proteine in bakterien
ATE294869T1 (de) * 1997-08-22 2005-05-15 Roche Diagnostics Gmbh Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
CA2485703A1 (en) * 2002-05-24 2003-12-04 Fred W. Wagner Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
CA2485695A1 (en) * 2002-05-24 2003-12-04 Fred W. Wagner Method for universal enzymatic production of bioactive peptides
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
PL2327718T3 (pl) 2003-11-21 2016-10-31 Ulepszone systemy ekspresyjne z wykorzystaniem systemu sekrecji SEC
EP1704234B1 (de) * 2003-11-21 2012-01-18 NPS Pharmaceuticals, Inc. Herstellung von glucagonähnlichem peptid 2 und analogen
KR20130019457A (ko) 2004-07-26 2013-02-26 다우 글로벌 테크놀로지스 엘엘씨 균주 조작에 의한 개선된 단백질 발현 방법
US7618799B2 (en) 2007-01-31 2009-11-17 Dow Global Technologies Inc Bacterial leader sequences for increased expression
MX2009011523A (es) 2007-04-27 2009-11-09 Dow Global Technologies Inc Metodo para clasificar rapidamente huespedes microbianos para identificar ciertas cepas con rendimiento y/o calidad mejorados en la expresion de proteinas heterologas.
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US8916358B2 (en) * 2010-08-31 2014-12-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
DE102011008075A1 (de) * 2011-01-07 2012-07-12 Universität Bayreuth Verfahren zur rekombinanten Herstellung von mehrfach Disulfid-verbrückten Peptiden
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
JP3205331B2 (ja) * 1989-03-14 2001-09-04 和光純薬工業株式会社 アクロモバクタープロテアーゼｉ類遺伝子及びその遺伝子産物
US5296773A (en) * 1993-04-20 1994-03-22 General Motors Corporation Composite rotor for a synchronous reluctance machine
DK52293D0 (de) * 1993-05-05 1993-05-05 Novo Nordisk As
US5843753A (en) * 1994-05-04 1998-12-01 Novo Nordisk A/S Metalloprotease having increased activity
EP0797671B1 (de) * 1994-12-09 2004-03-03 Novo Nordisk A/S Verfahren zur herstellung extrazellulärer proteine in bakterien
AU2091397A (en) * 1996-03-12 1997-10-01 Novo Nordisk A/S Novel achromobacter lyticus protease variants

Also Published As

Publication number Publication date
EP0797671B1 (de) 2004-03-03
JPH10511845A (ja) 1998-11-17
DE69532646T2 (de) 2005-03-03
JP3920331B2 (ja) 2007-05-30
ATE260979T1 (de) 2004-03-15
WO1996017943A1 (en) 1996-06-13
AU4114596A (en) 1996-06-26
EP0797671A1 (de) 1997-10-01
US6171823B1 (en) 2001-01-09

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8332 No legal effect for de
8370 Indication related to discontinuation of the patent is to be deleted
8364 No opposition during term of opposition