DE69531197D1 - Verdrängungschromatographie von proteinen mittels verdrängungsreagentien von geringem molekulargewicht - Google Patents

Verdrängungschromatographie von proteinen mittels verdrängungsreagentien von geringem molekulargewicht

Info

Publication number
DE69531197D1
DE69531197D1 DE69531197T DE69531197T DE69531197D1 DE 69531197 D1 DE69531197 D1 DE 69531197D1 DE 69531197 T DE69531197 T DE 69531197T DE 69531197 T DE69531197 T DE 69531197T DE 69531197 D1 DE69531197 D1 DE 69531197D1
Authority
DE
Germany
Prior art keywords
displacement
proteins
molecular weight
low molecular
reagents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69531197T
Other languages
English (en)
Other versions
DE69531197T2 (de
Inventor
Steven M Cramer
James A Moore
Amitava Kundu
Yufei Li
Guhan Jayaraman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE69531197D1 publication Critical patent/DE69531197D1/de
Publication of DE69531197T2 publication Critical patent/DE69531197T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C215/00—Compounds containing amino and hydroxy groups bound to the same carbon skeleton
    • C07C215/02—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C215/40—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton with quaternised nitrogen atoms bound to carbon atoms of the carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
DE69531197T 1994-02-16 1995-02-16 Verdrängungschromatographie von proteinen mittels verdrängungsreagentien von geringem molekulargewicht Expired - Lifetime DE69531197T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US197146 1994-02-16
US08/197,146 US5478924A (en) 1994-02-16 1994-02-16 Displacement chromatography of proteins using low molecular weight displacers
PCT/US1995/001966 WO1995022555A1 (en) 1994-02-16 1995-02-16 Displacement chromatography of proteins using low molecular weight displacers

Publications (2)

Publication Number Publication Date
DE69531197D1 true DE69531197D1 (de) 2003-08-07
DE69531197T2 DE69531197T2 (de) 2004-07-01

Family

ID=22728236

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69531197T Expired - Lifetime DE69531197T2 (de) 1994-02-16 1995-02-16 Verdrängungschromatographie von proteinen mittels verdrängungsreagentien von geringem molekulargewicht

Country Status (7)

Country Link
US (2) US5478924A (de)
EP (1) EP0751952B1 (de)
JP (1) JP3995260B2 (de)
CA (1) CA2183160C (de)
DE (1) DE69531197T2 (de)
NO (1) NO317346B1 (de)
WO (1) WO1995022555A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5478924A (en) * 1994-02-16 1995-12-26 Cramer; Steven M. Displacement chromatography of proteins using low molecular weight displacers
EP0840893B1 (de) * 1996-01-19 1999-09-15 Cohesive Technologies Inc. Hochleistungsflüssigchromatographieverfahren und vorrichtung
US6225352B1 (en) 1996-08-14 2001-05-01 Warner-Lambert Company Low molecular weight dendritic compounds as pharmaceutical agents
US6239262B1 (en) * 1998-01-07 2001-05-29 Rensselaer Polytechnic Institute Low molecular weight displacers for protein purification in hydrophobic interaction and reversed phase chromatographic systems
EP0943632A1 (de) * 1998-03-18 1999-09-22 Ecole Polytechnique Federale De Lausanne Quaternäres Ammoniumpolymer, seine Herstellung, seine Verwendung in die Trennung von Biomolekülen
US6573373B1 (en) * 1999-06-29 2003-06-03 Isis Pharmaceuticals, Inc. High affinity, low molecular weight displacers for oligonucleotide purification
US20050176937A1 (en) * 2000-02-23 2005-08-11 Rensselaer Polytechnic Institute High throughput screening of potential displacer molecules
US6881540B2 (en) 2000-02-23 2005-04-19 Rensselaer Polytechnic Institute High throughtput screening of potential displacer molecules
US6929747B2 (en) 2002-02-28 2005-08-16 Renesselaer Polytechnic Institute High-affinity, low-molecular-mass displacers for ion-exchange chromatography
US6749749B2 (en) 2002-06-26 2004-06-15 Isco, Inc. Separation system, components of a separation system and methods of making and using them
US7291395B2 (en) * 2004-02-18 2007-11-06 Dionex Corporation Coated ion exchanged substrate and method of forming
WO2006028433A1 (en) * 2004-09-07 2006-03-16 Dyer Gordon W Use of compounds for the inhibition of proteins and uv protection
DE112006001012B4 (de) * 2005-04-22 2017-05-18 Waters Technologies Corp. (N.D.Ges.D. Staates Delaware) Verfahren für eine auf Massenspektrometrie gerichtete Aufbereitung von Biopolymeren
WO2007053235A1 (en) * 2005-11-04 2007-05-10 Sachem, Inc. Cation-exchange displacement chromatography process and cationic organic compounds for use as displacer compounds in cation-exchange displacement chromatography process
EP1960078B1 (de) * 2005-12-02 2014-03-12 Sachem, Inc. Anionenaustausch-verdrängungschromatographieverfahren
KR101310815B1 (ko) * 2006-03-16 2013-09-27 에치투엘 주식회사 중공사막 및 그의 제조방법
CL2007002615A1 (es) * 2006-09-08 2008-04-18 Wyeth Corp Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto.
RU2768003C2 (ru) 2013-03-08 2022-03-22 Джензим Корпорейшн Интегрированное непрерывное производство терапевтических белковых лекарственных веществ
CN105960170A (zh) * 2013-12-23 2016-09-21 X微生物有限责任公司 杀微生物聚合物及其使用方法
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
BR112021003420A2 (pt) 2018-08-31 2021-05-18 Genzyme Corporation resina cromatográfica estéril e uso da mesma em processos de manufatura

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2946757A (en) * 1955-05-02 1960-07-26 George B Butler Quaternary ammonium ion exchange resins of improved properties
US3651231A (en) * 1969-09-10 1972-03-21 Upjohn Co Process for preparing lactogenic factor
US4352919A (en) * 1981-02-02 1982-10-05 Milliken Research Corporation Aminoalkoxypentaerythritols
US5156835A (en) * 1987-01-30 1992-10-20 Colgate-Palmolive Company Antibacterial antiplaque oral composition
US5028696A (en) * 1988-10-28 1991-07-02 Torres Anthony R Ion exchange and separation method
IT1232311B (it) * 1989-09-04 1992-01-28 Sclavo Spa Metodo di purificazione di composti a struttura peptidica o pseudo peptidica a basso peso molecolare.
US5478924A (en) * 1994-02-16 1995-12-26 Cramer; Steven M. Displacement chromatography of proteins using low molecular weight displacers

Also Published As

Publication number Publication date
EP0751952A1 (de) 1997-01-08
CA2183160A1 (en) 1995-08-24
NO317346B1 (no) 2004-10-18
JP3995260B2 (ja) 2007-10-24
WO1995022555A1 (en) 1995-08-24
JPH09509178A (ja) 1997-09-16
US5606033A (en) 1997-02-25
DE69531197T2 (de) 2004-07-01
EP0751952B1 (de) 2003-07-02
US5478924A (en) 1995-12-26
NO963420L (no) 1996-08-15
EP0751952A4 (de) 1997-12-17
CA2183160C (en) 1999-12-21

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Legal Events

Date Code Title Description
8332 No legal effect for de
8370 Indication of lapse of patent is to be deleted