DE69511903T2 - System zur kontinnierlichen räumlichen Abbildung für Abtastmikroskopie - Google Patents

System zur kontinnierlichen räumlichen Abbildung für Abtastmikroskopie

Info

Publication number
DE69511903T2
DE69511903T2 DE69511903T DE69511903T DE69511903T2 DE 69511903 T2 DE69511903 T2 DE 69511903T2 DE 69511903 T DE69511903 T DE 69511903T DE 69511903 T DE69511903 T DE 69511903T DE 69511903 T2 DE69511903 T2 DE 69511903T2
Authority
DE
Germany
Prior art keywords
image
scanning microscopy
imaging system
spatial imaging
continuous spatial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69511903T
Other languages
English (en)
Other versions
DE69511903D1 (de
Inventor
Jeffrey H Price
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE69511903D1 publication Critical patent/DE69511903D1/de
Publication of DE69511903T2 publication Critical patent/DE69511903T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microscoopes, Condenser (AREA)
  • Automatic Focus Adjustment (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Eye Examination Apparatus (AREA)
  • Image Processing (AREA)
  • Closed-Circuit Television Systems (AREA)
DE69511903T 1994-07-01 1995-06-30 System zur kontinnierlichen räumlichen Abbildung für Abtastmikroskopie Expired - Fee Related DE69511903T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27001794A 1994-07-01 1994-07-01

Publications (2)

Publication Number Publication Date
DE69511903D1 DE69511903D1 (de) 1999-10-07
DE69511903T2 true DE69511903T2 (de) 2000-04-20

Family

ID=23029550

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69511903T Expired - Fee Related DE69511903T2 (de) 1994-07-01 1995-06-30 System zur kontinnierlichen räumlichen Abbildung für Abtastmikroskopie
DE69508248T Expired - Lifetime DE69508248T2 (de) 1994-07-01 1995-06-30 Autofokussystem für abtastmikroskopie

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69508248T Expired - Lifetime DE69508248T2 (de) 1994-07-01 1995-06-30 Autofokussystem für abtastmikroskopie

Country Status (11)

Country Link
EP (2) EP0769159B1 (de)
JP (1) JPH10502466A (de)
AT (2) ATE177539T1 (de)
AU (1) AU2960395A (de)
CA (1) CA2192986C (de)
DE (2) DE69511903T2 (de)
DK (2) DK0769159T3 (de)
ES (2) ES2140178T3 (de)
GR (1) GR3031737T3 (de)
MX (1) MX9606683A (de)
WO (1) WO1996001438A1 (de)

Cited By (6)

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DE10115309A1 (de) * 2001-03-28 2002-10-02 Gnothis Holding Sa Ecublens Mikroskopanordnung zur Fluoreszenzspektorskopie, insbesondere Fluoreszenzkorrelationsspektroskopie
DE102011007751A1 (de) * 2011-04-20 2012-10-25 Carl Zeiss Microimaging Gmbh Weitfeldmikroskop und Verfahren zur Weitfeldmikroskopie
US9145589B2 (en) 2007-02-05 2015-09-29 Intelligent Biosystems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US9828632B2 (en) 2007-02-05 2017-11-28 Intelligent Bio-Systems, Inc. Detection device and methods of use
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches

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US6640014B1 (en) * 1999-01-22 2003-10-28 Jeffrey H. Price Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy
US7151847B2 (en) 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
US6743576B1 (en) 1999-05-14 2004-06-01 Cytokinetics, Inc. Database system for predictive cellular bioinformatics
US6651008B1 (en) 1999-05-14 2003-11-18 Cytokinetics, Inc. Database system including computer code for predictive cellular bioinformatics
EP1190271B1 (de) * 1999-06-04 2003-09-03 Janssen Pharmaceutica N.V. Robustes autofokussystem für mikroskop
DE60045076D1 (de) * 1999-08-10 2010-11-18 Cellavision Ab Verfahren und Vorrichtungen in einem optischen System
US6583865B2 (en) * 2000-08-25 2003-06-24 Amnis Corporation Alternative detector configuration and mode of operation of a time delay integration particle analyzer
AU2002232892B2 (en) 2000-10-24 2008-06-26 Intrexon Corporation Method and device for selectively targeting cells within a three -dimensional specimen
US7218764B2 (en) 2000-12-04 2007-05-15 Cytokinetics, Inc. Ploidy classification method
EP1368689B1 (de) 2001-02-02 2006-06-14 Cellomics, Inc. Verfahren zur schätzung der besten anfangsfokussierung
US6956961B2 (en) 2001-02-20 2005-10-18 Cytokinetics, Inc. Extracting shape information contained in cell images
US7016787B2 (en) 2001-02-20 2006-03-21 Cytokinetics, Inc. Characterizing biological stimuli by response curves
DE50212392D1 (de) * 2001-09-11 2008-07-31 Leica Microsystems Verfahren und vorrichtung zur optischen untersuchung eines objektes
DE10234404B4 (de) 2002-07-29 2021-10-14 Leica Microsystems Cms Gmbh Verfahren, Anordnung und Software zur Überwachung und Kontrolle eines Mikroskops
DE10362244B4 (de) * 2003-04-29 2014-06-26 Carl Zeiss Microscopy Gmbh Verfahren zur Bestimmung der Fokusposition und der Verkippung der Fokusebene bei der Abbildung einer Probe
DE10319182B4 (de) 2003-04-29 2008-06-12 Carl Zeiss Jena Gmbh Verfahren und Anordnung zur Bestimmung der Fokusposition bei der Abbildung einer Probe
EP1646926A2 (de) 2003-07-18 2006-04-19 Cytokinetics, Inc. Charakterisierung biologischer stimuli durch ansprechkurven
US7235353B2 (en) 2003-07-18 2007-06-26 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
US20050014217A1 (en) 2003-07-18 2005-01-20 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
US7232221B2 (en) 2004-02-24 2007-06-19 Searete, Llc Volumetric imaging using “virtual” lenslets
US8169534B2 (en) 2004-02-24 2012-05-01 The Invention Science Fund I, Llc Volumetric imaging using “virtual” lenslets
EP3410395B1 (de) * 2004-05-27 2020-03-11 Leica Biosystems Imaging, Inc. System und verfahren zur bewertung der bildqualität virtueller objektträger
US7323318B2 (en) 2004-07-15 2008-01-29 Cytokinetics, Inc. Assay for distinguishing live and dead cells
US7417213B2 (en) 2005-06-22 2008-08-26 Tripath Imaging, Inc. Apparatus and method for rapid microscopic image focusing having a movable objective
US20070031056A1 (en) 2005-08-02 2007-02-08 Perz Cynthia B System for and method of focusing in automated microscope systems
CN101438147B (zh) 2006-05-31 2011-09-28 奥林巴斯株式会社 生物试样摄像方法及生物试样摄像装置
DE102006055987B4 (de) * 2006-11-24 2011-06-01 Pentacon Gmbh Foto- Und Feinwerktechnik Verfahren zur Fokussierung optischer Bilderfassungseinrichtungen
US20090185734A1 (en) * 2008-01-18 2009-07-23 Hemocue Ab Apparatus and method for analysis of particles in a liquid sample
DE102008015885A1 (de) * 2008-03-26 2009-10-01 Synentec Gmbh Vorrichtung und Verfahren zur Autofokussierung von optischen Geräten, insbesondere von Mikroskopen
DE102009045107A1 (de) * 2009-09-29 2011-03-31 Carl Zeiss Imaging Solutions Gmbh Automatisches Fokussierverfahren für ein optisches Instrument zum vergrößerten Betrachten eines Objektes
US8774488B2 (en) 2010-03-11 2014-07-08 Cellscape Corporation Method and device for identification of nucleated red blood cells from a maternal blood sample
WO2011161594A1 (en) * 2010-06-24 2011-12-29 Koninklijke Philips Electronics N.V. Autofocus based on differential measurements
US9522396B2 (en) 2010-12-29 2016-12-20 S.D. Sight Diagnostics Ltd. Apparatus and method for automatic detection of pathogens
US20140125776A1 (en) * 2011-05-25 2014-05-08 Huron Technologies International Inc, 3d pathology slide scanner
CN104169719B (zh) 2011-12-29 2017-03-08 思迪赛特诊断有限公司 用于检测生物样品中病原体的方法和系统
EP2999988A4 (de) 2013-05-23 2017-01-11 S.D. Sight Diagnostics Ltd. Verfahren und system zur bildgebung einer zellprobe
IL227276A0 (en) 2013-07-01 2014-03-06 Parasight Ltd A method and system for obtaining a monolayer of cells, for use specifically for diagnosis
US10831013B2 (en) 2013-08-26 2020-11-10 S.D. Sight Diagnostics Ltd. Digital microscopy systems, methods and computer program products
DE102014104430B4 (de) 2014-03-28 2015-10-29 Jenoptik Optical Systems Gmbh Objektiv mit axialem Verstellglied zur aktiven Verstellung von Objektivelementen
JP6219214B2 (ja) 2014-03-31 2017-10-25 富士フイルム株式会社 細胞撮像制御装置および方法並びにプログラム
CN107077732B (zh) 2014-08-27 2020-11-24 思迪赛特诊断有限公司 用于对数字显微镜计算聚焦变化的系统及方法
CN114674825A (zh) 2015-09-17 2022-06-28 思迪赛特诊断有限公司 用于检测身体样本中实体的方法和设备
US9939623B2 (en) * 2015-10-19 2018-04-10 Molecular Devices, Llc Microscope system with transillumination-based autofocusing for photoluminescence imaging
JP6578928B2 (ja) * 2015-12-16 2019-09-25 コニカミノルタ株式会社 蛍光画像の合焦位置特定システム、合焦位置特定方法および合焦位置特定プログラム
CA3018536A1 (en) 2016-03-30 2017-10-05 S.D. Sight Diagnostics Ltd Distinguishing between blood sample components
US11099175B2 (en) 2016-05-11 2021-08-24 S.D. Sight Diagnostics Ltd. Performing optical measurements on a sample
EP4177593A1 (de) 2016-05-11 2023-05-10 S.D. Sight Diagnostics Ltd. Probenträger für optische messungen
WO2018097707A1 (en) * 2016-11-25 2018-05-31 Teledyne Dalsa B.V. Method for reconstructing a 2d image from a plurality of x-ray images
EP3351992B1 (de) * 2017-01-24 2019-10-23 Horiba France SAS Mikrospektrometriemessverfahren und -system
EP3396430B1 (de) 2017-04-27 2023-08-16 Euroimmun Medizinische Labordiagnostika AG Optische abtastanordnung und verfahren
JP7214729B2 (ja) 2017-11-14 2023-01-30 エス．ディー．サイト ダイアグノスティクス リミテッド 光学測定用試料収容器
AU2018375358B2 (en) * 2017-11-28 2021-02-04 Leica Biosystems Imaging, Inc. Dual processor image processing
GB2600996A (en) 2020-11-17 2022-05-18 Ffei Ltd Image scanning apparatus and method

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10115309A1 (de) * 2001-03-28 2002-10-02 Gnothis Holding Sa Ecublens Mikroskopanordnung zur Fluoreszenzspektorskopie, insbesondere Fluoreszenzkorrelationsspektroskopie
US9145589B2 (en) 2007-02-05 2015-09-29 Intelligent Biosystems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US9791409B2 (en) 2007-02-05 2017-10-17 Intelligent Biosystems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US9828632B2 (en) 2007-02-05 2017-11-28 Intelligent Bio-Systems, Inc. Detection device and methods of use
US10222349B2 (en) 2007-02-05 2019-03-05 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
DE112008000363B4 (de) 2007-02-05 2021-12-02 Qiagen Sciences, LLC (n.d.Ges.d. Staates Delaware) Vorrichtung zur Detektion und ihre Verwendung
US11371092B2 (en) 2007-02-05 2022-06-28 Qiagen Sciences, Llc Detection device and methods of use
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
DE102011007751A1 (de) * 2011-04-20 2012-10-25 Carl Zeiss Microimaging Gmbh Weitfeldmikroskop und Verfahren zur Weitfeldmikroskopie
US9588328B2 (en) 2011-04-20 2017-03-07 Carl Zeiss Microscopy Gmbh Wide-field microscope and method for wide-field microscopy
DE102011007751B4 (de) 2011-04-20 2023-10-19 Carl Zeiss Microscopy Gmbh Weitfeldmikroskop und Verfahren zur Weitfeldmikroskopie

Also Published As

Publication number Publication date
DE69508248T2 (de) 1999-11-04
AU2960395A (en) 1996-01-25
ES2140178T3 (es) 2000-02-16
ATE184113T1 (de) 1999-09-15
DE69508248D1 (de) 1999-04-15
EP0769159B1 (de) 1999-03-10
WO1996001438A1 (en) 1996-01-18
ES2131838T3 (es) 1999-08-01
CA2192986A1 (en) 1996-01-18
DK0769159T3 (da) 1999-09-27
EP0834758B1 (de) 1999-09-01
EP0834758A3 (de) 1998-05-06
ATE177539T1 (de) 1999-03-15
EP0834758A2 (de) 1998-04-08
GR3031737T3 (en) 2000-02-29
MX9606683A (es) 1997-12-31
DK0834758T3 (da) 1999-12-13
EP0769159A1 (de) 1997-04-23
CA2192986C (en) 2002-01-22
JPH10502466A (ja) 1998-03-03
DE69511903D1 (de) 1999-10-07

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