DE69511389T2 - System für Gelelektrophorese - Google Patents

System für Gelelektrophorese

Info

Publication number
DE69511389T2
DE69511389T2 DE69511389T DE69511389T DE69511389T2 DE 69511389 T2 DE69511389 T2 DE 69511389T2 DE 69511389 T DE69511389 T DE 69511389T DE 69511389 T DE69511389 T DE 69511389T DE 69511389 T2 DE69511389 T2 DE 69511389T2
Authority
DE
Germany
Prior art keywords
gel electrophoresis
electrophoresis
gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69511389T
Other languages
English (en)
Other versions
DE69511389D1 (de
Inventor
Sheldon Engelhorn
Timothy Updyke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
NOVEX
Novex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22830063&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69511389(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by NOVEX, Novex Inc filed Critical NOVEX
Application granted granted Critical
Publication of DE69511389D1 publication Critical patent/DE69511389D1/de
Publication of DE69511389T2 publication Critical patent/DE69511389T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69511389T 1994-03-31 1995-03-29 System für Gelelektrophorese Expired - Lifetime DE69511389T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/221,939 US5578180A (en) 1994-03-31 1994-03-31 System for PH-neutral longlife precast electrophoresis gel
PCT/US1995/003647 WO1995027197A1 (en) 1994-03-31 1995-03-29 System for ph-neutral longlife precast electrophoresis gel

Publications (2)

Publication Number Publication Date
DE69511389D1 DE69511389D1 (de) 1999-09-16
DE69511389T2 true DE69511389T2 (de) 2000-04-20

Family

ID=22830063

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69511389T Expired - Lifetime DE69511389T2 (de) 1994-03-31 1995-03-29 System für Gelelektrophorese

Country Status (8)

Country Link
US (5) US5578180A (de)
EP (1) EP0753142B1 (de)
JP (4) JP3679117B2 (de)
KR (1) KR100368955B1 (de)
AU (1) AU698145B2 (de)
CA (1) CA2186120C (de)
DE (1) DE69511389T2 (de)
WO (1) WO1995027197A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7422670B2 (en) 1994-03-31 2008-09-09 Timothy V Updyke System for pH-neutral stable electrophoresis gel

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US6143154A (en) * 1994-03-31 2000-11-07 Novex System for PH-neutral stable electrophoresis gel
US5578180A (en) * 1994-03-31 1996-11-26 Novel Experimental Technology System for PH-neutral longlife precast electrophoresis gel
SE9404141D0 (sv) * 1994-11-30 1994-11-30 Pharmacia Biotech Ab New buffer system for electrophoresis
US7824532B2 (en) * 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
ES2308800T3 (es) * 1997-01-08 2008-12-01 Invitrogen Corporation Metodos para la produccion de proteinas.
US6464850B1 (en) 1998-07-31 2002-10-15 Biowhittaker Molecular Applications, Inc. Method for producing hydrophilic monomers and uses thereof
US6758953B2 (en) 1999-10-28 2004-07-06 Nathan A. Thomas Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes
JP3942001B2 (ja) * 1999-12-02 2007-07-11 ハイモ株式会社 電気泳動用ポリアクリルアミドプレキャストゲル，その製造方法及び蛋白質の分離分析方法
EP1167962B1 (de) 1999-12-02 2014-01-08 Hymo Corporation Vorgegossene Polyacrylamidgele, Verfahren zu deren Herstellung und diese Gele verwendendes elektrophoretisches Verfahren
AUPQ571400A0 (en) * 2000-02-18 2000-03-16 Life Therapeutics Limited Improved electrophoresis gels
AU759336B2 (en) * 2000-02-18 2003-04-10 Dycent Biotech (Shanghai) Co., Limited Improved electrophoresis gels
US20040014082A1 (en) * 2000-08-11 2004-01-22 Invitrogen Corporation Highly homogeneous molecular markers for electrophoresis
US6562213B1 (en) 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
US20020134680A1 (en) * 2001-03-08 2002-09-26 Shmuel Cabilly Apparatus and method for electrophoresis
EP2196799A3 (de) * 2001-07-19 2010-08-25 Applera Corporation Puffer zur Elektrophorese und deren Verwendung
SE0201655D0 (sv) * 2002-05-31 2002-05-31 Amersham Biosciences Ab A method of electrophoresis
WO2004025259A2 (en) 2002-09-12 2004-03-25 Molecular Probes, Inc. Site-specific labeling of affinity tags in fusion proteins
US7850835B2 (en) * 2003-05-09 2010-12-14 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
US20050121325A1 (en) * 2003-09-19 2005-06-09 Timothy Updyke Composite compositions for electrophoresis
EP1664754A4 (de) * 2003-09-22 2010-08-25 Life Technologies Corp Vorrichtung zur gleichzeitigen elektrophorese in mehreren gelen
US7781173B2 (en) 2003-09-25 2010-08-24 Life Technologies Corporation Homogeneous populations of molecules
US20050103629A1 (en) * 2003-10-07 2005-05-19 Tom Diller Isoelectric focusing gels and methods of use thereof
US20060118418A1 (en) * 2004-12-02 2006-06-08 Mathoor Sivaram Electrophoresis gels and buffers and methods of performing electrophoresis
EP1853696B1 (de) 2005-02-24 2019-04-17 Life Technologies (Israel) Ltd. Vorrichtungen, systeme und kits zum elektroblotting sowie verfahren zu ihrer verwendung
WO2008042495A2 (en) 2006-07-21 2008-04-10 Life Technologies Corporation Sharply resolving labeled protein molecular weight standards
AU2008290079A1 (en) * 2007-08-17 2009-02-26 Hymo Corporation Precast gel for electrophoresis, method for producing the same, and use of the same
JP4838358B2 (ja) * 2007-08-17 2011-12-14 ハイモ株式会社 電気泳動用プレキャストゲル、その製造方法及びその使用方法
US20100044229A1 (en) * 2008-07-11 2010-02-25 Life Technologies Corporation Electrophoretically Enhanced Detection of Analytes on a Solid Support
US8282800B2 (en) 2008-09-02 2012-10-09 Bio-Rad Laboratories, Inc. Hydrolysis-resistant polyacrylamide gels
US8945360B2 (en) * 2009-01-27 2015-02-03 Bio-Rad Laboratories, Inc. High-performing electrophoresis gels with extended shelf lives
JP5619165B2 (ja) * 2009-08-24 2014-11-05 ライフ テクノロジーズ コーポレーション 高速高分離能のゲル電気泳動のためのシステム
EP2564187A4 (de) * 2010-04-27 2014-12-10 Dgel Electrosystem Inc Elektrophoresepuffer für schnellere migration, verbesserte auflösung und verlängerte haltbarkeit
WO2013006961A1 (en) * 2011-07-14 2013-01-17 Dgel Electrosystem Inc. Electrophoresis buffer for extending the useful electrophoresis life of an electrophoresis gel
US9291596B2 (en) 2013-02-12 2016-03-22 Pierce Biotechnology, Inc. Electroblot transfer buffer
WO2014150275A1 (en) 2013-03-15 2014-09-25 Bio-Rad Laboratories, Inc. Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability
USD738527S1 (en) 2013-05-28 2015-09-08 Life Technologies Corporation Electroblotting apparatus
GB2544198B (en) 2014-05-21 2021-01-13 Integenx Inc Fluidic cartridge with valve mechanism
US10690627B2 (en) 2014-10-22 2020-06-23 IntegenX, Inc. Systems and methods for sample preparation, processing and analysis
JP7116734B2 (ja) * 2017-02-24 2022-08-10 ライフ テクノロジーズ コーポレーション 毛細管電気泳動システム、関連する装置、および関連する方法
CN112305048B (zh) * 2020-12-22 2021-03-30 苏桥生物(苏州)有限公司 Mes和sds缓冲液在降低毛细管电泳碎片峰中的应用
CN113325055A (zh) * 2021-04-22 2021-08-31 中国人民解放军空军军医大学 聚丙烯酰胺凝胶电泳缓冲系统及其应用

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US3948743A (en) * 1975-04-14 1976-04-06 Bio-Rad Laboratories Method for gel electrophoresis
US4139440A (en) * 1977-06-20 1979-02-13 Government Of The United States Electrofocusing in buffers
US4209373A (en) * 1979-06-01 1980-06-24 Corning Glass Works Acidic agar gel electrochromatography of hemoglobins
US4542200A (en) * 1982-02-19 1985-09-17 Fmc Corporation Polyacrylamide cross-linked with a polysaccharide resin as electrophoretic gel medium
CA1206666A (en) * 1982-02-19 1986-06-24 Samuel Nochumson Copolymer of acrylamide and polysaccharide resin as electrophoretic gel medium
US4504641A (en) * 1982-02-19 1985-03-12 Fmc Corporation Polyacrylamide cross-linked with a polysaccharide resin as electrophoretic gel medium
US4481094A (en) * 1982-05-17 1984-11-06 Techamerica Group, Inc. Stabilized polyacrylamide gels and system for SDS electrophoresis
JPS59212751A (ja) * 1983-05-19 1984-12-01 Fuji Photo Film Co Ltd 電気泳動媒体材料の製造方法
US4950708A (en) * 1988-08-29 1990-08-21 Bio-Rad Laboratories, Inc. Stable polyacrylamide gels containing chaotropic agents
JPH05505877A (ja) * 1990-08-29 1993-08-26 ミコフ，ブディン トリス―タウリネート―ホルメート緩衝系と好適な均一電気泳動プレートとを用いた均一ゲル状中でのｓｄｓ電気泳動による蛋白質分離
JP2588059B2 (ja) * 1990-11-19 1997-03-05 ハイモ株式会社 電気泳動用ポリアクリルアミドゲルの製造方法
US5120413A (en) * 1991-05-31 1992-06-09 Beckman Instruments, Inc. Analysis of samples utilzing capillary electrophoresis
JP3071926B2 (ja) * 1992-01-28 2000-07-31 第一化学薬品株式会社 ポリアクリルアミド系電気泳動用ゲルプレートの製造方法
US5213669A (en) * 1992-01-31 1993-05-25 Beckman Instruments, Inc. Capillary column containing a dynamically cross-linked composition and method of use
US5275708A (en) * 1992-03-20 1994-01-04 Thomas Jefferson University Cetyltrimethylammonium bromide gel electrophoresis
WO1994023092A1 (en) * 1993-03-26 1994-10-13 United States Biochemical Corporation Improved sequencing gel
US5578180A (en) * 1994-03-31 1996-11-26 Novel Experimental Technology System for PH-neutral longlife precast electrophoresis gel

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7422670B2 (en) 1994-03-31 2008-09-09 Timothy V Updyke System for pH-neutral stable electrophoresis gel
US7452453B2 (en) 1994-03-31 2008-11-18 Invitrogen Corporation System for pH-neutral stable electrophoresis gel
US7892409B2 (en) 1994-03-31 2011-02-22 Life Technologies Corporation System for pH-neutral stable electrophoresis gel
US7967966B2 (en) 1994-03-31 2011-06-28 Life Technologies Corporation System for pH-neutral stable electrophoresis gel

Also Published As

Publication number Publication date
US6162338A (en) 2000-12-19
CA2186120A1 (en) 1995-10-12
US6059948A (en) 2000-05-09
CA2186120C (en) 2005-06-21
AU2192695A (en) 1995-10-23
US5578180A (en) 1996-11-26
JP4055862B2 (ja) 2008-03-05
JP2007263976A (ja) 2007-10-11
EP0753142B1 (de) 1999-08-11
JP2004163442A (ja) 2004-06-10
KR100368955B1 (ko) 2003-04-11
AU698145B2 (en) 1998-10-22
JPH09512907A (ja) 1997-12-22
US6096182A (en) 2000-08-01
JP3934114B2 (ja) 2007-06-20
JP2006177986A (ja) 2006-07-06
EP0753142A1 (de) 1997-01-15
WO1995027197A1 (en) 1995-10-12
US5922185A (en) 1999-07-13
DE69511389D1 (de) 1999-09-16
JP3679117B2 (ja) 2005-08-03

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: INVITROGEN CORP.(N.D.GES.D.STAATES DELAWARE), CARL

8327 Change in the person/name/address of the patent owner

Owner name: LIFE TECHNOLOGIES CORP. (N.D.GES.D. STAATES DE, US