DE69424153T2 - Methode zur verbesserten herstellung einer produzierende zellinie durch verwendung eines fusionsprotokolls - Google Patents

Methode zur verbesserten herstellung einer produzierende zellinie durch verwendung eines fusionsprotokolls

Info

Publication number
DE69424153T2
DE69424153T2 DE69424153T DE69424153T DE69424153T2 DE 69424153 T2 DE69424153 T2 DE 69424153T2 DE 69424153 T DE69424153 T DE 69424153T DE 69424153 T DE69424153 T DE 69424153T DE 69424153 T2 DE69424153 T2 DE 69424153T2
Authority
DE
Germany
Prior art keywords
cell line
pct
production
improving
producing cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69424153T
Other languages
English (en)
Other versions
DE69424153D1 (de
Inventor
Jack Spira
Lars Adamsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Swedish Orphan Biovitrum AB
Original Assignee
Pharmacia and Upjohn AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia and Upjohn AB filed Critical Pharmacia and Upjohn AB
Publication of DE69424153D1 publication Critical patent/DE69424153D1/de
Application granted granted Critical
Publication of DE69424153T2 publication Critical patent/DE69424153T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Data Exchanges In Wide-Area Networks (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE69424153T 1993-02-16 1994-02-15 Methode zur verbesserten herstellung einer produzierende zellinie durch verwendung eines fusionsprotokolls Expired - Lifetime DE69424153T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9300509A SE9300509D0 (sv) 1993-02-16 1993-02-16 Peg treatment
PCT/SE1994/000123 WO1994019459A1 (en) 1993-02-16 1994-02-15 Method for obtaining an increased production of a producing celline by using a fusion protocol

Publications (2)

Publication Number Publication Date
DE69424153D1 DE69424153D1 (de) 2000-05-31
DE69424153T2 true DE69424153T2 (de) 2000-11-16

Family

ID=20388915

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69424153T Expired - Lifetime DE69424153T2 (de) 1993-02-16 1994-02-15 Methode zur verbesserten herstellung einer produzierende zellinie durch verwendung eines fusionsprotokolls

Country Status (14)

Country Link
US (1) US5607845A (de)
EP (1) EP0684992B1 (de)
JP (1) JPH08509363A (de)
AT (1) ATE192191T1 (de)
AU (1) AU671195B2 (de)
CA (1) CA2156181C (de)
DE (1) DE69424153T2 (de)
DK (1) DK0684992T3 (de)
ES (1) ES2147781T3 (de)
GR (1) GR3034006T3 (de)
NZ (1) NZ261936A (de)
PT (1) PT684992E (de)
SE (1) SE9300509D0 (de)
WO (1) WO1994019459A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9303601D0 (sv) * 1993-11-01 1993-11-01 Kabi Pharmacia Ab Improved cell cultivation method and medium
US10329594B1 (en) * 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4659663A (en) * 1984-10-01 1987-04-21 Board Of Regents, University Of Texas System Methods for isolating cell fusion products
US4822470A (en) * 1987-10-09 1989-04-18 Baylor College Of Medicine Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
US4832814A (en) * 1987-12-28 1989-05-23 E. I. Du Pont De Nemours And Company Electrofusion cell and method of making the same
GB8807271D0 (en) * 1988-03-26 1988-04-27 Preece A W Cell fusion apparatus
SE465222C5 (sv) * 1989-12-15 1998-02-10 Pharmacia & Upjohn Ab Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning
SE468050C (sv) * 1991-03-15 1998-04-27 Pharmacia & Upjohn Ab Rekombinant derivat av human faktor VIII

Also Published As

Publication number Publication date
AU671195B2 (en) 1996-08-15
JPH08509363A (ja) 1996-10-08
ES2147781T3 (es) 2000-10-01
AU6119794A (en) 1994-09-14
DK0684992T3 (da) 2000-09-18
EP0684992B1 (de) 2000-04-26
CA2156181C (en) 2004-09-21
GR3034006T3 (en) 2000-11-30
SE9300509D0 (sv) 1993-02-16
ATE192191T1 (de) 2000-05-15
NZ261936A (en) 1996-05-28
DE69424153D1 (de) 2000-05-31
WO1994019459A1 (en) 1994-09-01
CA2156181A1 (en) 1994-09-01
US5607845A (en) 1997-03-04
EP0684992A1 (de) 1995-12-06
PT684992E (pt) 2000-10-31

Similar Documents

Publication Publication Date Title
ATE123925T1 (de) Verfahren zur herstellung eines enzymhydrolysates.
DE69333433D1 (de) Verfahren zur in vitro kultivierung dendritischer vorläuferzellen und deren verwendung zur immunogen herstellung
TR199701543T1 (xx) Memelilerde h�cre k�lt�rlerinin �retti�i proteinlerin sialilasyonunun kontrol�
LU90855I2 (en) Proteine 1 osteogenique recombinante humaine: bmp-7 et ses d-riv-s pharmaceutiquement acceptables
ATE330002T1 (de) Verfahren zur herstellung von membranvesikeln
ATE193020T1 (de) Verfahren zur herstellung von immunoglobulin g konzentrat
NO991271L (no) Benmorfogenetiske Protein-16-sammensetninger (BMP-16)
EP0400958A3 (de) Lebende Impfstoffe
DK0764209T3 (da) Albuminekspression i gær
DE69635924D1 (de) Isolierung und züchtung von schwannzellen
DE69736391D1 (de) Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium
DE69231995D1 (de) Verfahren zur Herstellung/Sekretion eines Proteins durch einen transformiertenSchimmelpilz unter Verwendung von Expressions-/Sekretions- Regulationsregionenaus Aspergillus
ATE92720T1 (de) Verfahren zur modifizierung von molke.
ATE169641T1 (de) Verfahren zur herstellung von stärkeabbauprodukten
CN106563173A (zh) 一种脱细胞生物真皮材料及其制备方法和应用
ATE280222T1 (de) Alkaline protease, verfahren zu ihrer herstellung,anwendung derselben und diese herstellende mikroorganismen
DE69424153T2 (de) Methode zur verbesserten herstellung einer produzierende zellinie durch verwendung eines fusionsprotokolls
ATE342062T1 (de) Angiotensin ii zur verbesserung der befruchtung
DE59607050D1 (de) Verfahren zur herstellung von riboflavin mittels mikroorganismen mit veränderter isocitratlyase-aktivität
DE69535126D1 (de) Verfahren zur herstellung von sekundären metaboliten
DE69528183T2 (de) Verfahren zur herstellung eines brotaufstrichs
ATE211460T1 (de) Verfahren zur herstellung von substituierten anthrachinonen und deren verwendung zur herstellung von rheinen
ATE101521T1 (de) Ein reines faktor i-protein und verfahren zur herstellung.
DK0504291T3 (da) Nye proteiner med oncostatin M-aktivitet og fremgangsmåde til deres fremstilling
HUT58790A (en) Process for producing cultivating medium for cell-cultures

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: BIOVITRUM AB, STOCKHOLM, SE