DE69421618T2 - In situ extraktion mikrobieller dna - Google Patents

In situ extraktion mikrobieller dna

Info

Publication number
DE69421618T2
DE69421618T2 DE69421618T DE69421618T DE69421618T2 DE 69421618 T2 DE69421618 T2 DE 69421618T2 DE 69421618 T DE69421618 T DE 69421618T DE 69421618 T DE69421618 T DE 69421618T DE 69421618 T2 DE69421618 T2 DE 69421618T2
Authority
DE
Germany
Prior art keywords
microbial dna
situ extraction
dna
situ
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69421618T
Other languages
English (en)
Other versions
DE69421618D1 (de
Inventor
Scott Lawrence Rakestraw
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of DE69421618D1 publication Critical patent/DE69421618D1/de
Publication of DE69421618T2 publication Critical patent/DE69421618T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69421618T 1993-08-13 1994-08-04 In situ extraktion mikrobieller dna Expired - Lifetime DE69421618T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10744093A 1993-08-13 1993-08-13
PCT/US1994/008498 WO1995005461A1 (en) 1993-08-13 1994-08-04 In situ extraction of microbial dna

Publications (2)

Publication Number Publication Date
DE69421618D1 DE69421618D1 (de) 1999-12-16
DE69421618T2 true DE69421618T2 (de) 2000-05-31

Family

ID=22316646

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69421618T Expired - Lifetime DE69421618T2 (de) 1993-08-13 1994-08-04 In situ extraktion mikrobieller dna

Country Status (9)

Country Link
US (1) US5595876A (de)
EP (1) EP0713528B1 (de)
JP (1) JP2638684B2 (de)
AT (1) ATE186569T1 (de)
AU (1) AU7406194A (de)
CA (1) CA2169271A1 (de)
DE (1) DE69421618T2 (de)
IL (1) IL110463A0 (de)
WO (1) WO1995005461A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5777098A (en) * 1996-07-23 1998-07-07 University Of North Dakota Medical Education Research Foundation DNA purification procedure
JP3133020B2 (ja) * 1997-09-03 2001-02-05 アサマ化成株式会社 真核微生物用抗菌剤およびそれを用いる真核微生物の増殖抑制方法
US6265164B1 (en) * 1998-03-26 2001-07-24 Biochain Institute, Inc. Compositions and methods for directly and rapidly analyzing the biochemical components of microorganisms
CA2270023C (en) * 1998-04-28 2010-10-12 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
FR2780059B1 (fr) * 1998-06-17 2002-10-11 Bio Merieux Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus
JP2000262247A (ja) 1999-03-19 2000-09-26 Asama Kasei Kk 免疫調節剤、免疫調節食品および免疫調節飼料
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
EP1207208A3 (de) * 2000-11-15 2003-12-10 Becton Dickinson and Company Methode zur Erhaltung von Zellen und Nukleinsäurezielmolekülen
AU2003220999A1 (en) * 2002-02-25 2003-09-09 Qualicon, Inc. Process for preparing processed sample liquid solution for electrophoresis
US7374649B2 (en) * 2002-11-21 2008-05-20 E. I. Du Pont De Nemours And Company Dispersion of carbon nanotubes by nucleic acids
US7214484B2 (en) * 2002-12-17 2007-05-08 Sigma-Aldrich Co. Compositions and methods for nucleic acid extraction from biological samples
US8298758B2 (en) 2003-12-26 2012-10-30 Prima Meat Packers, Ltd. Method of multiplex microorganism detection
WO2009126099A1 (en) * 2008-04-10 2009-10-15 Denator Aktiebolag Device for storing a biological sample and for preparing the biological sample
WO2011022228A2 (en) * 2009-08-20 2011-02-24 Srisuda Dhamwichukorn Apparatus and method for enhanced disruption and extraction of intracellular materials from microbial cells
EP2591102B1 (de) 2010-07-07 2015-05-13 Diagon Kft. Verfahren zur spezifischen isolierung des dna-gesamtgehalts bakterieller erreger und kit für diesen zweck
KR101749419B1 (ko) 2015-10-15 2017-07-03 주식회사 어큐진 파라핀포매된 샘플로부터 dna를 추출하는 방법
EP3448998B1 (de) * 2016-04-27 2020-06-03 Gen-Probe Incorporated Reagens für blutzelllyse
US10428370B2 (en) * 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830969A (en) * 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
GB8427006D0 (en) * 1984-10-25 1984-11-28 Iq Bio Ltd Determination of chlamydia trachomatis
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
JPH01285858A (ja) * 1988-05-13 1989-11-16 Eiken Chem Co Ltd 抗酸菌の核酸配列測定用試薬およびこの試薬を用いる核酸配列測定法
WO1990002179A1 (en) * 1988-08-31 1990-03-08 Lifecodes Corporation Method for the isolation of dna
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
US5185242A (en) * 1991-06-24 1993-02-09 Becton Dickinson And Company Method for lysing mycobacteria using achromopeptidase
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
EP0552571A1 (de) * 1992-01-09 1993-07-28 Becton, Dickinson and Company Freigabe von intrazellularen Komponenten

Also Published As

Publication number Publication date
ATE186569T1 (de) 1999-11-15
DE69421618D1 (de) 1999-12-16
JPH09500793A (ja) 1997-01-28
EP0713528B1 (de) 1999-11-10
CA2169271A1 (en) 1995-02-23
AU7406194A (en) 1995-03-14
US5595876A (en) 1997-01-21
IL110463A0 (en) 1994-10-21
JP2638684B2 (ja) 1997-08-06
WO1995005461A1 (en) 1995-02-23
EP0713528A1 (de) 1996-05-29

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Legal Events

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