DE69403654D1 - Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen - Google Patents

Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen

Info

Publication number
DE69403654D1
DE69403654D1 DE69403654T DE69403654T DE69403654D1 DE 69403654 D1 DE69403654 D1 DE 69403654D1 DE 69403654 T DE69403654 T DE 69403654T DE 69403654 T DE69403654 T DE 69403654T DE 69403654 D1 DE69403654 D1 DE 69403654D1
Authority
DE
Germany
Prior art keywords
increasing
production
biologically active
recombinant proteins
active recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69403654T
Other languages
English (en)
Other versions
DE69403654T2 (de
Inventor
Francois C Baneyx
Anthony A Gatenby
Cathy E Kalbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of DE69403654D1 publication Critical patent/DE69403654D1/de
Publication of DE69403654T2 publication Critical patent/DE69403654T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69403654T 1993-03-29 1994-03-28 Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen Expired - Fee Related DE69403654T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3838093A 1993-03-29 1993-03-29
PCT/US1994/003148 WO1994023042A1 (en) 1993-03-29 1994-03-28 A method for enhancing the production of biologically active recombinant proteins

Publications (2)

Publication Number Publication Date
DE69403654D1 true DE69403654D1 (de) 1997-07-10
DE69403654T2 DE69403654T2 (de) 1997-12-11

Family

ID=21899615

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69403654T Expired - Fee Related DE69403654T2 (de) 1993-03-29 1994-03-28 Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen

Country Status (4)

Country Link
US (1) US5552301A (de)
EP (1) EP0695359B1 (de)
DE (1) DE69403654T2 (de)
WO (1) WO1994023042A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0748398A (ja) * 1993-08-03 1995-02-21 Nippon Oil Co Ltd 変性蛋白質の再生方法及び変性蛋白質再生剤
JP3656277B2 (ja) * 1995-05-17 2005-06-08 味の素株式会社 組換えｄｎａ法によるトランスグルタミナーゼの効率的製造法
CA2226399A1 (en) * 1997-03-19 1998-09-19 Hsp Research Institute, Inc. Escherichia coli mutant
US6677139B1 (en) * 1999-12-23 2004-01-13 Genecor International, Inc. Methods for production of proteins in host cells
US20040157289A1 (en) * 2002-09-06 2004-08-12 Salerno John C. Protein expression system
KR100530988B1 (ko) * 2003-03-14 2005-11-28 한국과학기술원 봉입체 결합 단백질을 코딩하는 유전자를 결핍시키거나 증폭시켜 목적 단백질을 제조하는 방법
CN101180308A (zh) * 2005-05-23 2008-05-14 韩国科学技术院 利用sHSPs制备目的蛋白的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4758512A (en) * 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
DK454286A (da) * 1985-09-27 1987-03-28 Smithkline Beckman Corp Fremgangsmaade til stabil ekspression af en fremmed polypeptidkodendesekvens samt hermed transformerede e. coli-celler
SE8505921D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab A method to export gene products to the growth medium of gram negative bacteria
US5158875A (en) * 1989-08-25 1992-10-27 Amgen Inc. Production of biologically active insulin-like growth factor i from high expression host cell systems

Also Published As

Publication number Publication date
WO1994023042A1 (en) 1994-10-13
EP0695359A1 (de) 1996-02-07
US5552301A (en) 1996-09-03
DE69403654T2 (de) 1997-12-11
EP0695359B1 (de) 1997-06-04

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee