DE69333669D1 - Mutagenese und selektionsmethoden in den selben wirtszellen - Google Patents
Mutagenese und selektionsmethoden in den selben wirtszellenInfo
- Publication number
- DE69333669D1 DE69333669D1 DE69333669T DE69333669T DE69333669D1 DE 69333669 D1 DE69333669 D1 DE 69333669D1 DE 69333669 T DE69333669 T DE 69333669T DE 69333669 T DE69333669 T DE 69333669T DE 69333669 D1 DE69333669 D1 DE 69333669D1
- Authority
- DE
- Germany
- Prior art keywords
- peptide
- protein molecule
- host cell
- mutagenesis
- host cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000002703 mutagenesis Methods 0.000 title 1
- 231100000350 mutagenesis Toxicity 0.000 title 1
- 238000010187 selection method Methods 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 102000004169 proteins and genes Human genes 0.000 abstract 5
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 150000007523 nucleic acids Chemical group 0.000 abstract 2
- 238000007792 addition Methods 0.000 abstract 1
- 230000006154 adenylylation Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000022811 deglycosylation Effects 0.000 abstract 1
- 230000030609 dephosphorylation Effects 0.000 abstract 1
- 238000006209 dephosphorylation reaction Methods 0.000 abstract 1
- 238000013467 fragmentation Methods 0.000 abstract 1
- 238000006062 fragmentation reaction Methods 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 230000013595 glycosylation Effects 0.000 abstract 1
- 238000006206 glycosylation reaction Methods 0.000 abstract 1
- 239000003102 growth factor Substances 0.000 abstract 1
- 230000033444 hydroxylation Effects 0.000 abstract 1
- 238000005805 hydroxylation reaction Methods 0.000 abstract 1
- 238000006317 isomerization reaction Methods 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
- 230000026731 phosphorylation Effects 0.000 abstract 1
- 238000006366 phosphorylation reaction Methods 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Eye Examination Apparatus (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Saccharide Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US85241292A | 1992-03-16 | 1992-03-16 | |
US852412 | 1992-03-16 | ||
PCT/US1993/002349 WO1993019170A1 (en) | 1992-03-16 | 1993-03-09 | Selection methods |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69333669D1 true DE69333669D1 (de) | 2004-11-25 |
DE69333669T2 DE69333669T2 (de) | 2006-03-09 |
Family
ID=25313246
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69333669T Expired - Lifetime DE69333669T2 (de) | 1992-03-16 | 1993-03-09 | Mutagenese und selektionsmethoden in den selben wirtszellen |
Country Status (11)
Country | Link |
---|---|
US (3) | US6087177A (de) |
EP (2) | EP0631620B1 (de) |
JP (2) | JP3691846B2 (de) |
AT (1) | ATE280219T1 (de) |
AU (1) | AU687347B2 (de) |
CA (1) | CA2132306C (de) |
DE (1) | DE69333669T2 (de) |
ES (1) | ES2231769T3 (de) |
IL (1) | IL105038A0 (de) |
WO (1) | WO1993019170A1 (de) |
ZA (1) | ZA931835B (de) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6846628B1 (en) * | 1994-04-29 | 2005-01-25 | Jacob Nathaniel Wohlstadter | Selection methods |
US5914256A (en) * | 1995-06-07 | 1999-06-22 | Wohlstadter Jacob N | Method for promoting enzyme diversity |
ATE274594T1 (de) * | 1995-06-07 | 2004-09-15 | Jacob N Wohlstadter | Methode zur erhöhung der enzymdiversität |
US5919681A (en) * | 1995-06-07 | 1999-07-06 | Wohlstadter Jacob N | Method for promoting enzyme diversity |
US6506602B1 (en) | 1996-03-25 | 2003-01-14 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
DE69740033D1 (de) | 1996-07-03 | 2010-12-09 | Merial Inc | Rekombinanter hunde-adenovirus 2 (cav2), welcher exogene dna enthält |
US20070009930A1 (en) * | 1996-12-18 | 2007-01-11 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US7148054B2 (en) * | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
AU743305C (en) | 1997-01-17 | 2006-03-30 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
EP1036198B1 (de) | 1997-12-08 | 2012-09-26 | California Institute Of Technology | Verfahren zur Herstellung von Polynukleotid- und Polypeptidsequenzen |
US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
US6365410B1 (en) * | 1999-05-19 | 2002-04-02 | Genencor International, Inc. | Directed evolution of microorganisms |
JP2002136288A (ja) * | 2000-11-06 | 2002-05-14 | National Cancer Center-Japan | 酵母を用いた真核生物遺伝子の機能推定方法 |
CA2450577A1 (en) * | 2001-06-21 | 2003-01-03 | Diversa Corporation | Methods for the manufacture of pure single enantiomer compounds and for selecting enantioselective enzymes |
US20040137547A1 (en) * | 2001-12-28 | 2004-07-15 | Medtronic Minimed, Inc. | Method for formulating a glucose oxidase enzyme with a desired property or properties and a glucose oxidase enzyme with the desired property |
CN110546256A (zh) * | 2016-12-20 | 2019-12-06 | 彼得·B·雷因茨 | 通过调控突变率的定向进化 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1186660A3 (de) * | 1985-03-30 | 2002-03-20 | KAUFFMAN, Stuart A. | Verfahren zum Erhalten von DNA, RNA, Peptiden, Polypeptiden oder Proteinen durch ein DNA-Rekombinations-Verfahren |
AU637969B2 (en) * | 1987-10-07 | 1993-06-17 | Washington University | A method of maintaining a desired recombinant gene in a genetic population of cells |
US5096815A (en) * | 1989-01-06 | 1992-03-17 | Protein Engineering Corporation | Generation and selection of novel dna-binding proteins and polypeptides |
FI88309C (fi) * | 1989-04-20 | 1993-04-26 | Bio Orbit Oy | Ny biotest |
US5236836A (en) * | 1989-04-25 | 1993-08-17 | Igen, Inc. | Autoantibodies which enhance the rate of a chemical reaction |
US5283173A (en) * | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
AU7742691A (en) * | 1990-04-13 | 1991-11-11 | Schering Corporation | Novel protease assays |
US5258289A (en) * | 1990-09-05 | 1993-11-02 | Davis Claude G | Method for the selecting of genes encoding catalytic antibodies |
AU8740491A (en) * | 1990-09-28 | 1992-04-28 | Protein Engineering Corporation | Proteinaceous anti-dental plaque agents |
-
1993
- 1993-03-09 WO PCT/US1993/002349 patent/WO1993019170A1/en active IP Right Grant
- 1993-03-09 AT AT93907504T patent/ATE280219T1/de active
- 1993-03-09 EP EP93907504A patent/EP0631620B1/de not_active Expired - Lifetime
- 1993-03-09 JP JP51665493A patent/JP3691846B2/ja not_active Expired - Lifetime
- 1993-03-09 DE DE69333669T patent/DE69333669T2/de not_active Expired - Lifetime
- 1993-03-09 CA CA2132306A patent/CA2132306C/en not_active Expired - Lifetime
- 1993-03-09 EP EP04017517A patent/EP1477558A1/de not_active Withdrawn
- 1993-03-09 ES ES93907504T patent/ES2231769T3/es not_active Expired - Lifetime
- 1993-03-09 AU AU38089/93A patent/AU687347B2/en not_active Expired
- 1993-03-12 IL IL105038A patent/IL105038A0/xx unknown
- 1993-03-15 ZA ZA931835A patent/ZA931835B/xx unknown
-
1994
- 1994-04-29 US US08/235,437 patent/US6087177A/en not_active Expired - Lifetime
-
1995
- 1995-05-23 US US08/447,515 patent/US6162640A/en not_active Expired - Lifetime
- 1995-06-07 US US08/485,324 patent/US6043093A/en not_active Expired - Lifetime
-
2005
- 2005-04-19 JP JP2005121219A patent/JP2005270109A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP0631620B1 (de) | 2004-10-20 |
EP1477558A1 (de) | 2004-11-17 |
ZA931835B (en) | 1993-10-05 |
AU687347B2 (en) | 1998-02-26 |
CA2132306C (en) | 2012-07-10 |
EP0631620A1 (de) | 1995-01-04 |
ATE280219T1 (de) | 2004-11-15 |
DE69333669T2 (de) | 2006-03-09 |
US6162640A (en) | 2000-12-19 |
JP2005270109A (ja) | 2005-10-06 |
CA2132306A1 (en) | 1993-09-30 |
AU3808993A (en) | 1993-10-21 |
ES2231769T3 (es) | 2005-05-16 |
WO1993019170A1 (en) | 1993-09-30 |
US6043093A (en) | 2000-03-28 |
JPH08501201A (ja) | 1996-02-13 |
US6087177A (en) | 2000-07-11 |
EP0631620A4 (de) | 1997-11-12 |
JP3691846B2 (ja) | 2005-09-07 |
IL105038A0 (en) | 1993-07-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8327 | Change in the person/name/address of the patent owner |
Owner name: WOHLSTADTER, JACOB N., GAITHERSBURG, MD., US |
|
8381 | Inventor (new situation) |
Inventor name: INVENTOR IS APPLICANT |
|
8364 | No opposition during term of opposition |