DE69228098T2 - Verfahren zur Synthese von stabiler, einzelsträndiger cDNS in Eukaryoten durch Transfektion mit einem bakteriellen "Retron" enthaltenden Vektor und der verwendete Vektor - Google Patents

Verfahren zur Synthese von stabiler, einzelsträndiger cDNS in Eukaryoten durch Transfektion mit einem bakteriellen "Retron" enthaltenden Vektor und der verwendete Vektor

Info

Publication number
DE69228098T2
DE69228098T2 DE69228098T DE69228098T DE69228098T2 DE 69228098 T2 DE69228098 T2 DE 69228098T2 DE 69228098 T DE69228098 T DE 69228098T DE 69228098 T DE69228098 T DE 69228098T DE 69228098 T2 DE69228098 T2 DE 69228098T2
Authority
DE
Germany
Prior art keywords
retron
eukaryotes
transfection
synthesis
stable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69228098T
Other languages
English (en)
Other versions
DE69228098D1 (de
Inventor
Shohei Miyata
Atsushi Ohshima
Sumiko Inouye
Masayori Inouye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Medicine and Dentistry of New Jersey
Rutgers State University of New Jersey
Original Assignee
University of Medicine and Dentistry of New Jersey
Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Medicine and Dentistry of New Jersey, Rutgers State University of New Jersey filed Critical University of Medicine and Dentistry of New Jersey
Publication of DE69228098D1 publication Critical patent/DE69228098D1/de
Application granted granted Critical
Publication of DE69228098T2 publication Critical patent/DE69228098T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69228098T 1991-08-30 1992-08-28 Verfahren zur Synthese von stabiler, einzelsträndiger cDNS in Eukaryoten durch Transfektion mit einem bakteriellen "Retron" enthaltenden Vektor und der verwendete Vektor Expired - Fee Related DE69228098T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/753,110 US5436141A (en) 1989-02-24 1991-08-30 Method for synthesizing stable single-stranded CDNA in eukaryotes by means of a bacterial retron and products

Publications (2)

Publication Number Publication Date
DE69228098D1 DE69228098D1 (de) 1999-02-18
DE69228098T2 true DE69228098T2 (de) 1999-07-01

Family

ID=25029201

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69228098T Expired - Fee Related DE69228098T2 (de) 1991-08-30 1992-08-28 Verfahren zur Synthese von stabiler, einzelsträndiger cDNS in Eukaryoten durch Transfektion mit einem bakteriellen "Retron" enthaltenden Vektor und der verwendete Vektor

Country Status (5)

Country Link
US (1) US5436141A (de)
EP (1) EP0532380B1 (de)
JP (1) JP3140206B2 (de)
CA (1) CA2075515C (de)
DE (1) DE69228098T2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849563A (en) * 1989-02-24 1998-12-15 The University Of Medecine And Dentistry Of New Jersey Eukaryotes expressing single stranded hybrid molecules
US5780269A (en) * 1989-02-24 1998-07-14 The University Of Medicine And Denistry Of New Jersey Hybrid molecules
US6017737A (en) * 1989-02-24 2000-01-25 The University Of Medicine And Denistry Of New Jersey E. coli msDNA synthesizing system, products and uses
FR2675803B1 (fr) * 1991-04-25 1996-09-06 Genset Sa Oligonucleotides fermes, antisens et sens et leurs applications.
CA2151484A1 (en) 1992-12-09 1994-06-23 Jeffrey E. Miller Methods and compositions for cdna synthesis
US5366690A (en) * 1993-06-18 1994-11-22 Combustion Engineering, Inc. Zirconium alloy with tin, nitrogen, and niobium additions
US20070160581A1 (en) * 1994-04-29 2007-07-12 Cytogenix, Inc. Production of ssDNA in vivo
WO2000022114A1 (en) * 1998-10-09 2000-04-20 Ingene, Inc. PRODUCTION OF ssDNA $i(IN VIVO)
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
KR20010042069A (ko) 1998-03-20 2001-05-25 베니텍 오스트레일리아 리미티드 유전자 발현 조절방법
WO2000022113A1 (en) * 1998-10-09 2000-04-20 Ingene, Inc. ENZYMATIC SYNTHESIS OF ssDNA
AU2004205192B2 (en) * 1998-10-09 2007-09-20 Cytogenix, Inc. Production of ssDNA in vivo
US20030082800A1 (en) * 1998-10-09 2003-05-01 Cytogenix, Inc. In vivo ssDNA expression vectors for altering gene expression
DK1119615T3 (da) * 1998-10-09 2007-02-12 Cytogenix Inc Fremstilling af ssDNA in vivo
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
US7419964B2 (en) * 1999-09-16 2008-09-02 Cytogenix, Inc. Treatment of HSV-related pathologies using ssDNA
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna
AU2011253686C1 (en) * 2001-09-28 2014-04-03 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Micro-RNA molecules
EP2428568B1 (de) 2001-09-28 2018-04-25 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mikrorna-moleküle
WO2003093424A2 (en) * 2002-05-01 2003-11-13 Cytogenix, Inc. In vivo ssdna expression vectors for altering gene expression
US20050260588A1 (en) * 2002-05-01 2005-11-24 Conrad Charles A In vivo ssdna expression vectors for altering gene expression
EP1517992B1 (de) * 2002-06-05 2013-08-21 Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Retrons für gen-targeting
US20040248101A1 (en) * 2003-06-03 2004-12-09 Cytogenix, Inc. Identification of novel antibacteria agents by screening the single-stranded DNA expression library
US20050020526A1 (en) * 2003-06-03 2005-01-27 Cytogenix, Inc. Oligodeoxynucleotide intervention for prevention and treatment of sepsis
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2023183588A1 (en) * 2022-03-25 2023-09-28 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Methods of assessing engineered retron activity, and uses thereof
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0132309A3 (de) * 1983-06-27 1985-12-27 The Board Of Trustees Of The Leland Stanford Junior University Regulierbares eukaryotisches Promotorelement
US5079151A (en) * 1989-02-24 1992-01-07 University Of Medicine And Dentistry Of New Jersey Production of branched RNA-linked multi-copy single-stranded DNA using permeabilized cells

Also Published As

Publication number Publication date
CA2075515C (en) 2005-11-01
EP0532380B1 (de) 1999-01-07
CA2075515A1 (en) 1993-03-01
EP0532380A2 (de) 1993-03-17
DE69228098D1 (de) 1999-02-18
US5436141A (en) 1995-07-25
JP3140206B2 (ja) 2001-03-05
EP0532380A3 (de) 1994-05-04
JPH06217776A (ja) 1994-08-09

Similar Documents

Publication Publication Date Title
DE69228098D1 (de) Verfahren zur Synthese von stabiler, einzelsträndiger cDNS in Eukaryoten durch Transfektion mit einem bakteriellen "Retron" enthaltenden Vektor und der verwendete Vektor
DE69212342D1 (de) Verfahren zur Herstellung von polymerischen Schäumen und chemische Zusammensetzung zu dessen Herstellung
DE59306172D1 (de) Flammenhydrolytisch hergestelltes Titandioxid-Mischoxid, Verfahren zu seiner Herstellung und Verwendung
DE59306638D1 (de) Verfahren zur Herstellung von 2,2,6,6-Tetramethylpiperidin-N-oxyl und in seiner 4-Stellung substituierten Derivaten
DE69327764D1 (de) Verfahren zur Herstellung von granulösen Nahrungsmitteln
DE59200764D1 (de) Verfahren und Einrichtung zur Aufbereitung oder Herstellung von Kunststoffen.
DE69415936T2 (de) Verfahren zur Herstellung von schlagzähen Polyacetalharzzusammensetzungen
DE69204378D1 (de) Zusammensetzung zur Beschichtung und diesbezügliches Verfahren zur Umhüllung von gekörntem Düngemittel.
DE69212569D1 (de) Verfahren zur Herstellung von Legierungspulvern des SE-Fe/Co-B-M-Typs und gebundene Magnete mit diesem Legierungspulver
VN274A1 (en) 4-benzoylisoxazole compositions, process for preparing thereof, herbicide containing thereof and their use
DE69120041D1 (de) Kontinuierliches Verfahren zur Herstellung von Copolymeren aus konjugierten Dienen und vinylaromatischen Verbindungen
DE69418160T2 (de) Vorrichtung zur Herstellung eines kosmetischen Produktes auf eine dosierte Weise, und zum Spenden dieses Produktes
DE69318858T2 (de) Verfahren zur herstellung von duroplastischen, mesogenen, orientierten kunststoffen enthaltenden verbundmaterialien
DE69424343T2 (de) Kontinuierliches Verfahren zur Herstellung von gummimodifizierten Styrolharzen und dadurch erzeugte Produkte
DE69220837D1 (de) Copolymer auf Basis von Maleimid, Verfahren zu seiner Herstellung und dieses enthaltende thermoplastische Harzzusammensetzung
DE69205535D1 (de) Verfahren zur Herstellung von Phenylbutyronitrilen und Verwendung in der Parfümerie des 2,2-Dimethyl-4-Phenyl-Valeriannitrile.
DE59204049D1 (de) Verfahren zur Herstellung von Hydroxylammoniumsalzen.
DE69204467D1 (de) Verfahren zur Ansäuerung von Flüssigmist, in welchem Ammoniak gebunden ist und Kohlendioxyd freigesetzt wird.
DE3868686D1 (de) Nahrungsprodukt in form von garnelen und vorrichtung zu dessen herstellung.
DE69300221D1 (de) Kunststoffbehälter und verfahren zu seiner herstellung.
DE68911148D1 (de) Zusammensetzungen und verfahren zur herstellung von schaumkunststoffen.
DE69201245D1 (de) Verfahren zur Synthese von Hydrogenoperfluoralkanen.
DE59301021D1 (de) Verfahren zur kontinuierlichen Herstellung von 3-Cyano-3,5,5-trimethylcyclohexanon.
DE59300883D1 (de) Verfahren zur Herstellung von Dianthrachinon-N,N'-dihydroazin und dessen Chlorierungsprodukten.
DE59202454D1 (de) Verfahren zur Herstellung von TiO2-haltigen Pellets und deren Verwendung.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee