DE69127552D1 - Methode zum screening einer dna bibliothek - Google Patents

Methode zum screening einer dna bibliothek

Info

Publication number
DE69127552D1
DE69127552D1 DE69127552T DE69127552T DE69127552D1 DE 69127552 D1 DE69127552 D1 DE 69127552D1 DE 69127552 T DE69127552 T DE 69127552T DE 69127552 T DE69127552 T DE 69127552T DE 69127552 D1 DE69127552 D1 DE 69127552D1
Authority
DE
Germany
Prior art keywords
screening
dna library
homologous
methods
recombination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69127552T
Other languages
English (en)
Inventor
Douglas Treco
Allan Miller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shire Human Genetics Therapies Inc
Original Assignee
Transkaryotic Therapies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transkaryotic Therapies Inc filed Critical Transkaryotic Therapies Inc
Application granted granted Critical
Publication of DE69127552D1 publication Critical patent/DE69127552D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigation Of Foundation Soil And Reinforcement Of Foundation Soil By Compacting Or Drainage (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Superconductors And Manufacturing Methods Therefor (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Alcoholic Beverages (AREA)
DE69127552T 1991-08-02 1991-11-21 Methode zum screening einer dna bibliothek Expired - Lifetime DE69127552D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73986191A 1991-08-02 1991-08-02
PCT/US1991/008679 WO1993003183A1 (en) 1991-08-02 1991-11-21 Library screening method

Publications (1)

Publication Number Publication Date
DE69127552D1 true DE69127552D1 (de) 1997-10-09

Family

ID=24974078

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69127552T Expired - Lifetime DE69127552D1 (de) 1991-08-02 1991-11-21 Methode zum screening einer dna bibliothek

Country Status (12)

Country Link
EP (1) EP0596885B1 (de)
JP (1) JPH06508983A (de)
AT (1) ATE157708T1 (de)
AU (2) AU1278192A (de)
CA (1) CA2114713A1 (de)
DE (1) DE69127552D1 (de)
IE (1) IE914397A1 (de)
IL (1) IL100151A0 (de)
MX (1) MX9200096A (de)
NZ (1) NZ240749A (de)
PT (1) PT100753A (de)
WO (1) WO1993003183A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR930701625A (ko) * 1990-07-13 1993-06-12 리처드 에프. 셀든 라이브러리 스크리닝 방법
PT839211E (pt) * 1995-07-13 2001-10-30 Forschungszentrum Juelich Gmbh Processo para a preparacao de riboflavina por meio de microorganismos com actividade de isocitratoliase modificada
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
JP4771563B2 (ja) 1996-12-06 2011-09-14 アムジエン・インコーポレーテツド Il−1媒介疾患を処置するためにil−1インヒビターを使用する組合せ療法
US7220893B1 (en) 1999-05-19 2007-05-22 Cornell Research Foundation, Inc. Plasmids and methods for construction of non-redundant, indexed, saturation, gene-disruption plant and animal libraries

Also Published As

Publication number Publication date
PT100753A (pt) 1993-09-30
MX9200096A (es) 1993-02-01
EP0596885B1 (de) 1997-09-03
ATE157708T1 (de) 1997-09-15
EP0596885A1 (de) 1994-05-18
AU6211296A (en) 1996-12-05
NZ240749A (en) 1994-04-27
AU1278192A (en) 1993-03-02
WO1993003183A1 (en) 1993-02-18
CA2114713A1 (en) 1993-02-18
JPH06508983A (ja) 1994-10-13
IL100151A0 (en) 1992-08-18
AU693712B2 (en) 1998-07-02
IE914397A1 (en) 1993-02-10

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Legal Events

Date Code Title Description
8332 No legal effect for de