DE69023773D1 - Verfahren zur herstellung von polypeptiden in einem zellfreien translationssystem. - Google Patents

Verfahren zur herstellung von polypeptiden in einem zellfreien translationssystem.

Info

Publication number
DE69023773D1
DE69023773D1 DE69023773T DE69023773T DE69023773D1 DE 69023773 D1 DE69023773 D1 DE 69023773D1 DE 69023773 T DE69023773 T DE 69023773T DE 69023773 T DE69023773 T DE 69023773T DE 69023773 D1 DE69023773 D1 DE 69023773D1
Authority
DE
Germany
Prior art keywords
substrates
translation system
cell
translation
gtp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69023773T
Other languages
English (en)
Other versions
DE69023773T2 (de
Inventor
Sergei Jurievich Ovodov
Vladimir Ivanovich Baranov
July Borisovich Alakhov
Ljubov Anatolievna Ryabova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INST BELKA AKAD NAUK SSSR
Original Assignee
INST BELKA AKAD NAUK SSSR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INST BELKA AKAD NAUK SSSR filed Critical INST BELKA AKAD NAUK SSSR
Publication of DE69023773D1 publication Critical patent/DE69023773D1/de
Application granted granted Critical
Publication of DE69023773T2 publication Critical patent/DE69023773T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/18011—Details ssRNA Bacteriophages positive-sense
    • C12N2795/18111—Leviviridae
    • C12N2795/18122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE69023773T 1989-07-31 1990-06-05 Verfahren zur herstellung von polypeptiden in einem zellfreien translationssystem. Expired - Lifetime DE69023773T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SU4717700 1989-07-31
PCT/SU1990/000145 WO1991002075A1 (fr) 1989-07-31 1990-06-05 Procede d'obtention de polypeptides dans un systeme de translation exempt de cellules

Publications (2)

Publication Number Publication Date
DE69023773D1 true DE69023773D1 (de) 1996-01-04
DE69023773T2 DE69023773T2 (de) 1996-10-24

Family

ID=21460267

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69023773T Expired - Lifetime DE69023773T2 (de) 1989-07-31 1990-06-05 Verfahren zur herstellung von polypeptiden in einem zellfreien translationssystem.

Country Status (6)

Country Link
EP (1) EP0485608B1 (de)
JP (1) JP2891540B2 (de)
AT (1) ATE130633T1 (de)
CA (1) CA2064685C (de)
DE (1) DE69023773T2 (de)
WO (1) WO1991002075A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4930993A (en) * 1992-09-24 1994-04-12 Public Health Research Institute Of The City Of New York, Inc., The Coupled replication-translation methods and kits for protein synthesis
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
ATE368747T1 (de) * 2000-08-29 2007-08-15 Cellfree Sciences Co Ltd Verfahren zur synthese von zellfreien proteinen
CN1252261C (zh) * 2000-08-30 2006-04-19 株式会社无细胞科学 无细胞蛋白质合成用转录模板的设计、构建及使用其的稀释分批式麦胚芽无细胞蛋白质的合成法
WO2003064672A1 (fr) * 2002-01-31 2003-08-07 Yaeta Endo Extrait cellulaire pour synthese de proteine acellulaire et procede de production de cet extrait

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4382028A (en) * 1982-07-19 1983-05-03 Monsanto Company Separation of plasma proteins from cell culture systems
SU1618761A1 (ru) * 1987-04-29 1991-01-07 Институт Белка Ан Ссср Способ получени пептидов и белков в бесклеточной системе трансл ции

Also Published As

Publication number Publication date
EP0485608B1 (de) 1995-11-22
CA2064685A1 (en) 1991-02-01
ATE130633T1 (de) 1995-12-15
DE69023773T2 (de) 1996-10-24
EP0485608A1 (de) 1992-05-20
JP2891540B2 (ja) 1999-05-17
CA2064685C (en) 1996-06-18
EP0485608A4 (en) 1992-06-17
WO1991002075A1 (fr) 1991-02-21
JPH05505095A (ja) 1993-08-05

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