DE68929192T2 - Elektrophorese unter Verwendung elektrischer Felder einer vorgegebenen Kontur - Google Patents

Elektrophorese unter Verwendung elektrischer Felder einer vorgegebenen Kontur

Info

Publication number
DE68929192T2
DE68929192T2 DE68929192T DE68929192T DE68929192T2 DE 68929192 T2 DE68929192 T2 DE 68929192T2 DE 68929192 T DE68929192 T DE 68929192T DE 68929192 T DE68929192 T DE 68929192T DE 68929192 T2 DE68929192 T2 DE 68929192T2
Authority
DE
Germany
Prior art keywords
electrodes
electrophoresis
electric fields
field
clamped
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE68929192T
Other languages
English (en)
Other versions
DE68929192D1 (de
Inventor
Gilbert Chu
Douglas Vollrath
Ron Davis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Application granted granted Critical
Publication of DE68929192D1 publication Critical patent/DE68929192D1/de
Publication of DE68929192T2 publication Critical patent/DE68929192T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44713—Particularly adapted electric power supply
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Power Engineering (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Electrostatic Spraying Apparatus (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Electrostatic Separation (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE68929192T 1988-08-23 1989-08-21 Elektrophorese unter Verwendung elektrischer Felder einer vorgegebenen Kontur Expired - Fee Related DE68929192T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/235,296 US5165898A (en) 1986-08-13 1988-08-23 Electrophoresis using contour-clamped electric fields

Publications (2)

Publication Number Publication Date
DE68929192D1 DE68929192D1 (de) 2000-05-18
DE68929192T2 true DE68929192T2 (de) 2000-11-23

Family

ID=22884910

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68929192T Expired - Fee Related DE68929192T2 (de) 1988-08-23 1989-08-21 Elektrophorese unter Verwendung elektrischer Felder einer vorgegebenen Kontur

Country Status (8)

Country Link
US (1) US5165898A (de)
EP (1) EP0356187B1 (de)
JP (1) JP2744293B2 (de)
AT (1) ATE191787T1 (de)
CA (1) CA1340313C (de)
DE (1) DE68929192T2 (de)
ES (1) ES2147174T3 (de)
GR (1) GR3033906T3 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5549796A (en) * 1986-08-14 1996-08-27 The Board Of Trustees Of The Leland Stanford Junior University Electrophoresis using contour-clamped electric fields
US6808609B1 (en) 1990-02-28 2004-10-26 Aclara Biosciences, Inc. Device and method for moving charged particles
US5935401A (en) * 1996-09-18 1999-08-10 Aclara Biosciences Surface modified electrophoretic chambers
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5126022A (en) * 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5298143A (en) * 1991-10-25 1994-03-29 Washington State University Research Foundation Electrophoretic processing
GB2264783A (en) * 1992-02-24 1993-09-08 Gen Electric Co Plc Electrophoretic analysis method utilising wave effect
US5645702A (en) * 1995-06-07 1997-07-08 Hewlett-Packard Company Low voltage miniaturized column analytical apparatus and method
EP0745844A3 (de) * 1995-02-07 1998-03-04 Centro Nacional De Investigaciones Cientificas Vorrichtung zur Trennung von chromosomengrossen DNS-Molekülen durch Elektrophorese
AU6574601A (en) * 2000-06-07 2001-12-17 Centro Nacional De Investigaciones Cientificas (Cnic) Pulsed field electrophoresis chambers, accessories and method of utilization for seperation of DNA molecules
JP2004518663A (ja) * 2000-12-18 2004-06-24 トラスティーズ オブ プリンストン ユニバーシティ 非対称なパルスフィールド電気泳動を使用した巨大分子の分画
CU22855A1 (es) * 2001-06-08 2003-05-26 Cnic Ct Nac Investigaciones Circuito para imponer los voltajes en los electrodos de las cubetas del sistema chef de electroforesis de campos pulsantes
US7597791B2 (en) * 2001-10-19 2009-10-06 The Trustees Of Princeton University Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules
US6905585B2 (en) * 2002-09-11 2005-06-14 Temple University Of The Commonwealth System Of Higher Education Automated system for high-throughput electrophoretic separations
DE602004017443D1 (de) * 2003-04-14 2008-12-11 Caliper Life Sciences Inc Reduktion der migrations-shift-assay-interferenz
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US8133371B2 (en) * 2004-02-02 2012-03-13 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
WO2009094772A1 (en) 2008-02-01 2009-08-06 The University Of British Columbia Methods and apparatus for particle introduction and recovery
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
CA2742327A1 (en) 2011-05-20 2012-11-20 The University Of British Columiba Systems and methods for enhanced scoda
WO2013166444A2 (en) 2012-05-04 2013-11-07 Boreal Genomics Corp. Biomarker analysis using scodaphoresis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
EP3168290B1 (de) * 2014-07-07 2021-12-08 Logos Biosystems, Inc. Vorrichtung zur klärung von gewebe mittels elektrophorese
ES2936844T3 (es) * 2015-03-06 2023-03-22 4D Lifetec Ag Sistema de electroforesis en gel para electroforesis en gel unicelular
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
JP7038209B2 (ja) * 2017-11-13 2022-03-17 エフ．ホフマン－ラ ロシュ アーゲー エピタコ電気泳動を使用する試料分析のための装置
MX2022005183A (es) 2019-10-29 2022-08-08 Quantum Si Inc Bombeo peristáltico de fluidos y métodos, sistemas y dispositivos asociados.

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4148703A (en) * 1976-02-11 1979-04-10 Morton Weintraub Method of electrophoretic purification of enzymes and peptides by means of an adjustable, specialized, geometrically located electrode system
US4371746A (en) * 1978-01-05 1983-02-01 Peptek, Incorporated Edge terminations for impedance planes
US4315812A (en) * 1980-05-28 1982-02-16 Karlson Eskil L Apparatus for continuous electrochromatographic separation
US4473452A (en) * 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
US4670119A (en) * 1985-10-25 1987-06-02 Hurd Stanley M Isoelectric focusing device and process
DE3784983T2 (de) * 1986-08-13 1993-11-11 Univ Leland Stanford Junior Elektrophorese, welche homogene oder inhomogene elektrische Felder mit bestimmtem Umkreis benutzt.
FR2620230B1 (fr) * 1987-09-07 1990-02-23 Ari Sa Procede et dispositif pour l'electrophorese des macromolecules de grandes dimensions

Also Published As

Publication number Publication date
EP0356187A2 (de) 1990-02-28
US5165898A (en) 1992-11-24
ATE191787T1 (de) 2000-04-15
JP2744293B2 (ja) 1998-04-28
EP0356187A3 (de) 1991-10-09
EP0356187B1 (de) 2000-04-12
DE68929192D1 (de) 2000-05-18
ES2147174T3 (es) 2000-09-01
GR3033906T3 (en) 2000-11-30
CA1340313C (en) 1999-01-12
JPH02114169A (ja) 1990-04-26

Similar Documents

Publication Publication Date Title
DE68929192T2 (de) Elektrophorese unter Verwendung elektrischer Felder einer vorgegebenen Kontur
NO872232D0 (no) Fettinversjons-gelelektroforese.
TW345683B (en) Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
ATE175723T1 (de) Elektrochemische denatusierung von doppelsträngiger nukleinsäure
ATE106256T1 (de) Apparat zur regeneration von nerven bei säugern.
DE69632820D1 (de) Vorrichtung und verfahren zur elektrophorese
DE69314818D1 (de) Akkumulator mit nichtwässrigem Elektrolyt und Verfahren zur Herstellung von positivem Elektrodenmaterial
DE3874213D1 (de) Verfahren zur orientierung von fluessigkristallinen polymeren mittels elektrischer felder und mittels dieses verfahrens hergestellte orientierte materialien aus fluessigkristallinen polymeren.
DE69334211D1 (de) Multidrug resistenz gen
EP0365321A3 (de) Elektrophoresegerät
AT368400B (de) Zelle mit selektiv durchlaessigen membranen und ionenharzen und elektroden
DE69626984T2 (de) Ionenkanal
EP0256737A3 (en) Electrophoresis using contour-clamped homogeneous electric fields
MX26714A (es) Procedimiento para la preparacion de albumina de SUERO HUMANO VECTOR Y PLASMIDO USADOS ASI COMO LEVADURA METILOTROFICA TRANSFORMADA OBTENIDA
BR8401937A (pt) Processo de eletrolise e celula eletrolitica
EP0327363A3 (de) Trennung von grossen DNA-Molekülen in asymmetrischen elektrischen Wechselfeldern
DE3776505D1 (de) Gegendrehverfahren zum richten von profilen und richtmaschine zur anwendung dieses verfahrens.
EP0175371A3 (en) Method for silver staining proteins and nucleic acids
DE3686714D1 (de) Elektrophorese-verfahren und vorrichtung zur trennung von teilchen in einem trennmedium.
BR8806985A (pt) Processo de ajuste de eletrodos de celulas eletroliticas
ATE68104T1 (de) Kontinuierliches scheidungsverfahren durch elektrophorese und elektroosmose von elektrisch geladenen pulverisierten feststoffen.
DE3882220D1 (de) Herstellung von chlordioxyd in einer elektrolytischen zelle.
ATE101645T1 (de) Verfahren und anlage zur gewinnung von plasmiden und cosmiden.
AU8082794A (en) Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
AT380182B (de) Apparat zur elektrophorese mit polyakrylamidgel mit orientiertem stromfeld

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee