DE68928899D1 - Leadersequenz zur induzierung einer posttranslationalen modifikation von polypeptiden in bakterien, sowie dafür kodierendes gen - Google Patents

Leadersequenz zur induzierung einer posttranslationalen modifikation von polypeptiden in bakterien, sowie dafür kodierendes gen

Info

Publication number
DE68928899D1
DE68928899D1 DE68928899A DE68928899A DE68928899D1 DE 68928899 D1 DE68928899 D1 DE 68928899D1 DE 68928899 A DE68928899 A DE 68928899A DE 68928899 A DE68928899 A DE 68928899A DE 68928899 D1 DE68928899 D1 DE 68928899D1
Authority
DE
Germany
Prior art keywords
polypeptides
post
leader sequence
inducing
bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE68928899A
Other languages
German (de)
English (en)
Other versions
DE68928899T2 (de
DE68928899T4 (de
Inventor
Norman Hansen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland at Baltimore
University of Maryland at College Park
Original Assignee
University of Maryland at Baltimore
University of Maryland at College Park
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland at Baltimore, University of Maryland at College Park filed Critical University of Maryland at Baltimore
Application granted granted Critical
Publication of DE68928899D1 publication Critical patent/DE68928899D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE68928899A 1988-07-05 1989-06-30 Leadersequenz zur induzierung einer posttranslationalen modifikation von polypeptiden in bakterien, sowie dafür kodierendes gen Expired - Fee Related DE68928899D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/214,959 US5218101A (en) 1988-07-05 1988-07-05 Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor
PCT/US1989/002820 WO1990000558A1 (en) 1988-07-05 1989-06-30 Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor

Publications (1)

Publication Number Publication Date
DE68928899D1 true DE68928899D1 (de) 1999-02-18

Family

ID=22801074

Family Applications (2)

Application Number Title Priority Date Filing Date
DE68928899T Expired - Lifetime DE68928899T4 (de) 1988-07-05 1989-06-30 Leadersequenz zur induzierung einer posttranslationalen modifikation von polypeptiden in bakterien, sowie dafür kodierendes gen
DE68928899A Expired - Fee Related DE68928899D1 (de) 1988-07-05 1989-06-30 Leadersequenz zur induzierung einer posttranslationalen modifikation von polypeptiden in bakterien, sowie dafür kodierendes gen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE68928899T Expired - Lifetime DE68928899T4 (de) 1988-07-05 1989-06-30 Leadersequenz zur induzierung einer posttranslationalen modifikation von polypeptiden in bakterien, sowie dafür kodierendes gen

Country Status (14)

Country Link
US (1) US5218101A (no)
EP (1) EP0423224B1 (no)
JP (2) JP3326172B2 (no)
KR (1) KR0137961B1 (no)
AT (1) ATE175417T1 (no)
AU (1) AU640110B2 (no)
CA (1) CA1339414C (no)
DE (2) DE68928899T4 (no)
DK (1) DK191D0 (no)
IL (1) IL90832A (no)
NO (1) NO309200B1 (no)
NZ (1) NZ229538A (no)
WO (1) WO1990000558A1 (no)
ZA (1) ZA894721B (no)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516682A (en) * 1988-07-05 1996-05-14 University Of Maryland Subtilin variant of enhanced stability and activity
US5348881A (en) * 1990-03-13 1994-09-20 Quest International Flavors & Good Ingredients Company, Division Of Indopco, Inc. Multiple bacteriocin producing lactococcus and compositions
NL9000753A (nl) * 1990-03-30 1991-10-16 Nl Zuivelonderzoek Inst Dna-fragment, ten minste coderend voor een deel van het extracellulaire eiwit met een schijnbaar molecuulgewicht van 60 kda van lactococcus lactis stammen zoals de lactococcus lactis ssp. lactis stam mg1363, combinaties hiervan met voor homologe of heterologe eiwitten coderende dna-sequenties alsmede de hiermee verkregen homologe en heterologe eiwitten.
DE69232930T2 (de) * 1991-04-11 2003-12-11 Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo), Ede Nisin a aehnliche lantibiotika, diese produzierende milchsaeurebakterien und deren herstellung
US5861376A (en) * 1991-07-01 1999-01-19 Quest International Flavors & Food Ingredients Company, Division Of Indopco, Inc. Synthetically derived peptide
GB9207267D0 (en) * 1992-04-02 1992-05-13 Agricultural & Food Res Nisins
DE69433357T2 (de) * 1994-09-12 2004-09-09 Aventis Pharma Deutschland Gmbh Rekombinantes Mersacidin und Verfahren zu seiner Herstellung
US5919695A (en) * 1997-04-22 1999-07-06 Quest International, B.V. Method and culture for inhibiting undesired microorganisms with culture of Bacillus subtilis
US6953683B2 (en) * 1997-07-18 2005-10-11 University Of Maryland Compositions and methods of inhibiting bacterial spore germination
AU8484798A (en) * 1997-07-18 1999-02-10 University Of Maryland Sublancin lantibiotic produced by (bacillus subtilis) 168
EP1140975A4 (en) * 1998-12-24 2003-01-08 Univ Maryland SUBTILINE STABLE AND METHODS OF PREPARING SAME
US6759205B2 (en) 2000-06-29 2004-07-06 University Of Maryland Construction of a strain of Bacillus subtilis 168 that displays the sublancin lantibiotic on the surface of the cell
US6846804B2 (en) * 2000-06-29 2005-01-25 University Of Maryland, College Park Office Of Technology Commercialization Construction of a structural variant of sublancin to facilitate its isolation and use in bioremediation of environmental contamination by gram-positive spore formers such as bacillus anthrasis
US7033766B2 (en) * 2000-06-29 2006-04-25 University Of Maryland Office Of Technology Commercialization Construction and screening of lantibody display libraries
WO2003063887A1 (en) * 2002-01-14 2003-08-07 University Of Maryland, College Park Compositions and method of inhibiting bacterial spore germination
US6861236B2 (en) * 2002-05-24 2005-03-01 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way
CN101115838B (zh) 2004-12-07 2015-08-12 莫菲西斯公司 产生和分泌修饰的肽的方法
BRPI0707782A2 (pt) * 2006-02-13 2011-05-10 Boehringer Ingelheim Vetmed processo para a fabricaÇço de lantibiàticos
US7923533B2 (en) * 2006-06-29 2011-04-12 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7816101B2 (en) * 2006-06-29 2010-10-19 The Invention Science Fund I, Llc Apparatus for arbitrary peptide synthesis
US7879975B2 (en) * 2006-06-29 2011-02-01 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7910695B2 (en) * 2006-06-29 2011-03-22 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4716115A (en) * 1983-09-06 1987-12-29 Microlife Technics, Inc. Derived nisin producing microorganisms, method of production and use and products obtained thereby

Also Published As

Publication number Publication date
JPH03505670A (ja) 1991-12-12
JP3326172B2 (ja) 2002-09-17
IL90832A0 (en) 1990-01-18
NO905638D0 (no) 1990-12-28
EP0423224A1 (en) 1991-04-24
DK191A (da) 1991-01-02
JP3418388B2 (ja) 2003-06-23
ATE175417T1 (de) 1999-01-15
NO905638L (no) 1990-12-28
WO1990000558A1 (en) 1990-01-25
US5218101A (en) 1993-06-08
DE68928899T2 (de) 1999-08-12
NO309200B1 (no) 2000-12-27
KR0137961B1 (ko) 1998-04-30
IL90832A (en) 1995-06-29
KR900701819A (ko) 1990-12-04
ZA894721B (en) 1990-03-28
JP2002191383A (ja) 2002-07-09
EP0423224B1 (en) 1999-01-07
EP0423224A4 (en) 1991-11-06
DE68928899T4 (de) 1999-11-25
AU3968489A (en) 1990-02-05
DK191D0 (da) 1991-01-02
CA1339414C (en) 1997-09-02
NZ229538A (en) 1992-01-29
AU640110B2 (en) 1993-08-19

Similar Documents

Publication Publication Date Title
DE68928899D1 (de) Leadersequenz zur induzierung einer posttranslationalen modifikation von polypeptiden in bakterien, sowie dafür kodierendes gen
ES2031806T3 (es) Factor de permeabilidad vascular humano.
NL300062I2 (nl) Osteogene middelen.
ATE138102T1 (de) Verfahren zur herstellung von cysteinfreien peptiden
DK1019073T3 (da) Apoliprotein A-I agonister og deres anvendelse til at behandle dyslipidæmiske lidelser
ES2120964T3 (es) Peptidos osteogenicos.
ATE188994T1 (de) Methoden zur trans-destabilisierung von spezifischen proteinen in vivo
PT81445A (de) Verfahren zur spaltung von peptiden und proteinen an der methionyl-bindung
ATE193208T1 (de) Verwendung synthetischer peptide zur induktion der toleranz gegen t- und b-zell epitope von autoantigenen
DE3881023D1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.
DE3873273D1 (de) Verfahren zur selektiven spaltung von fusionsproteinen.
ATE113986T1 (de) Verfahren zur herstellung von aprotinin und aprotinin-homologen in hefe.
DE68923496D1 (de) Verfahren zur Herstellung von motilinähnlichen Polypeptiden und seine Expression.
ATE141645T1 (de) In vitro-behandlung von fusionsproteinen
DE59006998D1 (de) Verfahren zur herstellung von hirudin.
ES2033212A6 (es) Procedimiento para la obtencion de peptidos puramente glicosilados, parcialmente glicosilados o no glicosilados.
DE58907746D1 (de) Verfahren zur Bestimmung von Fructosamin.
DK0540560T3 (da) Ancrod-lignende proteiner, fremstilling og anvendelse deraf
DE60138885D1 (de) Polypeptide mit aktivität der stimulierung von neutrophilen
ATE76081T1 (de) Verfahren zur herstellung von dipeptiden mit nterminalen nichtproteinogenen aminosaeuren.
ATE79651T1 (de) Rekombinante materialien und verfahren zur herstellung von menschliche konnektive gewebe aktivierende peptide iii und deren analoge.
DE3485883D1 (de) Rekombinante materialien und verfahren zur herstellung von menschliche konnektive gewebe aktivierende peptide iii und deren analoge.
ATE102950T1 (de) Verfahren zur herstellung von dipeptiden mit cterminalen nicht-proteinogenen aminosaeuren.
ATE91303T1 (de) Verfahren zur herstellung von peptiden durch spezifische spaltung von gentechnisch gewonnenen fusionsproteinen mit collagenasen.
ATE128717T1 (de) Schizosaccharomyces-spezifische polypeptide.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee