DE6753413U - LIGHTING DEVICE FOR FLUORESCENCE MICROSCOPES - Google Patents

Description

P.A. 409 835 * 7/15/68

Patent-Treuhand-Gesellschaft for electric light bulbs BbH, Munich

"Illumination device for fluorescence microscopes"

The innovation relates to a lighting device for fluorescence microscopes with at least one discharge lamp as the light source, in particular for the investigation of phosphors used in lighting technology and for use in analytical chemistry as a supplement the UV analysis lamp in the microscopic range.

The fluorescence microscopy served so far essentially to make microscopic To examine preparations in the field of medicine, food analysis, forensics and the like. The objects to be examined are thereby either by short-wave light immediately or after treatment with fluorescent substances to emit fluorescent light stimulated.

The optical structure of the lighting device has been customary up to now Fluorescence microscopes looked so that the high-pressure mercury discharge diarrhea or the like set as a realized point light source in a reflector and the emanating from the reflector Rays were directed through a condenser for fluoroscopy onto the object.

*) 42 h 14/05 - / -

In fluorescence microscopy, one comes to the requirement of a point light source in the beam path of the lighting device inevitably to high or extremely high pressure lamps and thus to limit the wavelength of the exciting radiation downwards. A fluorescence microscopy below 300 nm was so far only possible with great effort.

In contrast, the task of the innovation is to investigate For phosphors which can be excited by shorter UV radiation, a lighting device specially suitable for this purpose as an additional device for any microscope.

For this purpose, the lighting device mentioned at the beginning is in accordance with the innovation characterized in that at least one low-pressure metal vapor discharge lamp with a high proportion of radiation at wavelengths around 250 nm is provided as dark field lighting.

By using a low-pressure metal halide discharge lamp with a high proportion of radiation at wavelengths of us 250 ns in the lighting device With a fluorescence microscope, it is now possible for the first time to use phosphors that are produced by short-wave UV radiation can be excited below 300 nm. as A low-pressure metal vapor discharge lamp is particularly suitable for this purpose with a mercury low-pressure discharge lamp high radiation component at 254 nm.

If you choose the mercury low-pressure discharge lamp as a ring lamp, so the electrode ends should be set as sharp as possible, so that a practically completely circular radiation source is achieved.

To achieve the effect of the dark field lighting in accordance with the innovation the ring lamp is placed above or below the microscope

λ ι

Table arranged so that no direct light from the lamp enters the observation doorway. The effect of dark field lighting, which is usually only achieved by condensers specially designed for this purpose, is achieved with the suitably mounted ring lamp without any additional equipment. Due to the high proportion of 254 nm radiation from the Hg low-pressure lamp, neither an excitation filter nor a UV blocking filter is necessary for dark field lighting. The use of a filter which is transparent to 254 nm and blocks visible light would make a fluorescence illumination device very expensive.

For the investigation of phosphors, fluorescent minerals and for ¥ ^ Tven-a * m§ in aef fluorescence analysis in analytical chemistry, a device for observing the afterglow is also provided. This device makes it possible, for example, to identify phases in mixtures of phosphors that have luminescent times of different lengths. For this purpose, the excitation laser si ^ - operated only one half-wave of an alternating current. By a synchoni- * is ** 4.s Blssde, vi «eg Lgehsiscneige acngexritten by a synchronous motor, in the observation beam path the afterglow object is observed only in the afterglow phase.

To clarify the lighting device according to ύ & τ innovation - »ground two versions explained in the drawings by way of example *

It shows

FIG. 1 shows a side view of a fluorescence microscope with one Bing lamp comprehensive innovation lighting device,

FIG. 2 in a plan view from below in an enlarged representation, the neuemngsgeaäSe ring lamp under the microscope table of the Microscope of Figtrr 1,

Figure 3 shows the section along the line III-III according to Figure 2 "

FIG. 4 shows a side view of another fluorescence microscope with the additional device for observing the Afterglow.

In Figures 1 to 3, an embodiment of a fluorescence microscope for excitation at 254 nm is shown as the light source a low-pressure mercury discharge lamp 1 is provided in a ring shape. It is preferably a low-pressure mercury UV lamp with, for example, 10 Vatt power in the manner of disinfection lamps. The electrode ends 2 of the lamp vessel are set as sharply as possible at 3, thus creating a practically completely circular radiation source to enable. Of course, other suitable low-pressure discharge lamps can also be used Find application. For dark field illumination, the lamp is at such a distance under the microscope = table 4 mounted so that no direct light enters the observation beam path, but can only fall on the object. The slide 5 consists of a plastic frame 6 on the a cover glass 7 is cemented on.

The material to be examined 8, e.g. a fluorescent powder with particles about iO / µm in size are below that of the light source facing side of the cover glass 7 applied. The application is easy with the help of a drop of relaxed water.

The ring lamp can also be arranged above the microscope table to achieve reflected light illumination. If you want dark field observations enable, one must also ensure that no direct light enters the observation beam path, but only reaches the object. In this case too, observation in the afterglow phase is possible.

FIG. 4 shows an embodiment of a fluorescence microscope for the excitation at 254 nm, and for the observation during the afterglow of the phosphor. The excitation glass 1 is only a bit of a harbveil of a ¥ echseiaii-w «iti3 b £ t ~ i = efesn» Ssr synchronous motor 9 rotates the siren disc 10, the peripheral openings 11 of which then release the observation beam path when the excitation light has gone out. The phase position between the lamp and the siren disc is adjusted by turning the motor housing.

- Protection claims -

-j-

Claims (4)

EA.409835 * 15.7.68'J protection claims 1. Illumination device for fluorescence microscopes, especially for examining those which can be excited by UV radiation Phosphors with at least one metal vapor discharge lamp as the UV light source, characterized in that at least one low-pressure metal vapor discharge lamp (1) has high proportion of radiation at ¥ ¥ ellenlengths around 250 in is intended as dark field lighting. 2. Lighting device according to claim 1, characterized in that a low-pressure mercury discharge larape is provided as the low-pressure metal vapor discharge lamp « 3. Lighting device according to claim 2, characterized in that the low-pressure mercury discharge lamp is a ring lamp (l) with s ort arf attached electrode ends (2), the so above or below the microscope ^ table (4) is arranged that no direct light enters the observation beam path, but only reaches the object. 4. Device for observing the afterglow using of a lighting device according to Claims 1 to 3, characterized in that the excitation lamp is operated with only one half-wave of an alternating current, and the observation beam path is released by a synchronized aperture during the lamp's pause in darkness. Dr. Hb / Pa / Wg.