DE627028C - Process for obtaining physiologically active substances from muscles - Google Patents

Process for obtaining physiologically active substances from muscles

Info

Publication number
DE627028C
DE627028C DEI31356D DEI0031356D DE627028C DE 627028 C DE627028 C DE 627028C DE I31356 D DEI31356 D DE I31356D DE I0031356 D DEI0031356 D DE I0031356D DE 627028 C DE627028 C DE 627028C
Authority
DE
Germany
Prior art keywords
muscles
physiologically active
active substances
substances
solvents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEI31356D
Other languages
German (de)
Inventor
Dr Carl Ludwig Lautenschlaeger
Dr Willy Ludwig
Dr Otto Schaumann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IG Farbenindustrie AG
Original Assignee
IG Farbenindustrie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IG Farbenindustrie AG filed Critical IG Farbenindustrie AG
Priority to DEI31356D priority Critical patent/DE627028C/en
Application granted granted Critical
Publication of DE627028C publication Critical patent/DE627028C/en
Expired legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Cardiology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Vascular Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Description

Verfahren zur Gewinnung physiologisch wirksamer Substanzen aus Muskeln Das Patent 626 27,4 erstreckt sich auf ein Verfahren zur Gewinnung physiologisch wirksamer Substanzen aus Muskeln, wobei diese mit organischen Lösungsmitteln zweckmäßig in neutraler oder schwach alkalischer Reaktion extrahiert und die so gewonnenen Extrakte nach Entfernen der Ballaststoffe in wäßrige Lösung gebracht werden.Process for obtaining physiologically active substances from muscles The patent 626 27.4 extends to a method of obtaining physiological effective substances from muscles, these being expedient with organic solvents extracted in a neutral or weakly alkaline reaction and the thus obtained Extracts can be brought into aqueous solution after removing the dietary fiber.

In. weiterer Ausarbeitung des Verfahrens wurde nun die Beobachtung gemacht, daß sich die Rohkonzentrate durch Behandeln. mit wasserunlöslichen organischen Lösungsmitteln in saurer Lösung, in Lipoidlösungsmitteln lösliche und in nur wasserlösliche Stoffe zerlegen lassen. Die so getrennten und durch Entfernen der wasserunlöslichen Lösungsmittel und der Ballaststoffe weiter gereinigten Substanzen zeigen eine qualitativ verschiedene Wirkung, und zwar wirken die wasserlöslichen Stoffe auf die Reizleitung des isolierten Froschherzens, während die lipoidlöslichen Stoffe die Herzleistung nachhaltig steigern. Die in Lip;oidlösungsmitteln, wie Alkohol, Aceton, Äther usw., löslichen Stoffe sind frei von Phosphor und Schwefel. Sowohl die in Lipoidlösungsmitteln löslichen als auch die nur in Wasser löslichen Substanzen können als solche oder in geeigneten Lösungsmitteln gelöst zur Anwendung gelangen.In. Further elaboration of the procedure was now the observation made that the raw concentrates by treating. with water-insoluble organic Solvents in acidic solution, soluble in lipoid solvents and only soluble in water Let fabrics disassemble. The so separated and by removing the water-insoluble Solvent and the dietary fiber further purified substances show a qualitative different effects, namely the water-soluble substances act on the conduction of stimuli of the isolated frog heart, while the lipoid-soluble substances increase the cardiac output sustainably increase. Those in lipid solvents such as alcohol, acetone, ether, etc., Soluble substances are free from phosphorus and sulfur. Both those in lipoid solvents Soluble as well as the substances soluble only in water can be as such or be used dissolved in suitable solvents.

Beispiele i. 2 kg Kalbfleisch werden gemahlen und mit 2,5 1 Aceton + Zoo ccm n-Natronlauge 3 Stunden kalt extrahiert. Hierauf wird mit je 2 1 Aceton die Extraktion noch dreimal wiederholt. Der Gesamtextrakt wird bei - I2° C filtriert und im Vakuumeingedampft. Das Konzentrat wird mit Salzsäure schwach angesäuert und mit Äther extrahiert.Examples i. 2 kg of veal are ground and mixed with 2.5 1 of acetone + Zoo ccm of sodium hydroxide solution extracted cold for 3 hours. This is followed by 2 liters of acetone each repeat the extraction three more times. The total extract is filtered at - I2 ° C and evaporated in a vacuum. The concentrate is weakly acidified with hydrochloric acid and extracted with ether.

Der ätherlösliche Anteil wird zur Trockne eingedampft, der Rückstand mit trocknem Aceton aufgenommen, die Lösung ausgefroren und filtriert. Nach dem Eindampfen wird der hellgelbe pastenähnliche Rückstand in geeigneten Lösungsmitteln aufgenommen.The ether-soluble portion is evaporated to dryness, the residue taken up with dry acetone, the solution frozen out and filtered. After this The light yellow paste-like residue is evaporated in suitable solvents recorded.

Der wäßrige Extrakt ist eiweißfrei und sterilisierbar. Er kann sowohl in schwach saurer wie in schwach alkalischer Reaktion zur Anwendung gelangen.The aqueous extract is protein-free and sterilizable. He can do both be used in weakly acidic as well as in weakly alkaline reaction.

2. 2 kg Herz werden fettfrei präpariert, zerkleinert und mit 2,5 1 Aceton + 200 ccm n-Natronlauge 3 Stünden kalt extrahiert. Die Acetonextraktion wird dreimal mit je 21 Aceton wiederholt. Die weitere Behandlung geschieht wie in Beispiel i. Die letzten pasfieitähnlichen Rückstände können nach bekannten Methoden vom Cholesterin usw. befreit werden.2. 2 kg heart are prepared fat-free, minced and with 2.5 1 Acetone + 200 ccm sodium hydroxide solution extracted cold for 3 hours. The acetone extraction will repeated three times with 21% acetone each time. Further treatment happens as in example i. The last pasfieitäitähnlichen residues can after known Methods to be freed from cholesterol etc.

Claims (1)

PATENTANSPRUCH: . Abänderung des Verfahrens des Pateügs 626 274 zur Darstellung physiologisch wirksamer Substanzen, darin bestehend, daß man die nach dem Verfahren gewonnenen Rohkonzentratie aus Muskeln in saurer Lösung mit wasserunlöslichen organischen Lösungsmitteln behandelt und die so getrennten Stoffe von den Lösungsmitteln und Ballaststoffen befreit.PATENT CLAIM:. Modification of the procedure of Pateug 626 274 to Representation of physiologically active substances, consisting in that one after Raw concentrate obtained by the process from muscles in acidic solution with water-insoluble ones treated organic solvents and the so separated substances from the solvents and fiber exempt.
DEI31356D 1927-06-03 1927-06-03 Process for obtaining physiologically active substances from muscles Expired DE627028C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEI31356D DE627028C (en) 1927-06-03 1927-06-03 Process for obtaining physiologically active substances from muscles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEI31356D DE627028C (en) 1927-06-03 1927-06-03 Process for obtaining physiologically active substances from muscles

Publications (1)

Publication Number Publication Date
DE627028C true DE627028C (en) 1936-03-06

Family

ID=7187849

Family Applications (1)

Application Number Title Priority Date Filing Date
DEI31356D Expired DE627028C (en) 1927-06-03 1927-06-03 Process for obtaining physiologically active substances from muscles

Country Status (1)

Country Link
DE (1) DE627028C (en)

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