DE62237T1 - Verfahren zur feststellung der carcinogenen aktivitaet von chemischen oder physikalischen mitteln. - Google Patents

Verfahren zur feststellung der carcinogenen aktivitaet von chemischen oder physikalischen mitteln.

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Publication number
DE62237T1
DE62237T1 DE198282102458T DE82102458T DE62237T1 DE 62237 T1 DE62237 T1 DE 62237T1 DE 198282102458 T DE198282102458 T DE 198282102458T DE 82102458 T DE82102458 T DE 82102458T DE 62237 T1 DE62237 T1 DE 62237T1
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DE
Germany
Prior art keywords
dna
cells
carcinogenicity
transformed
cell line
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198282102458T
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English (en)
Inventor
Sara Kiryat Ono Lavi
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Yeda Research and Development Co Ltd
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Yeda Research and Development Co Ltd
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Filing date
Publication date
Application filed by Yeda Research and Development Co Ltd filed Critical Yeda Research and Development Co Ltd
Publication of DE62237T1 publication Critical patent/DE62237T1/de
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G01N33/5017—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity for testing neoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/91—Cell lines ; Processes using cell lines
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y10S436/813—Cancer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (1)

  1. VOSSIUS -VOSSIUS -TAUCH N Ε·=> - HEUNEMANN- RAUH
    PATENTANWÄLTE
    SIEBERTSTRASSE Λ ■ 8OO Q MÜNCHEN 86 · PHONE: (OBS) 47 4Ο75 CABLE: BENZOLP AT ENT MÖNCHEN ■ TELEX 5-29 45 3 VOPAT D
    u.Z.: R 742 EP
    Europäische Patentanmeldung Nr. 82102458.5
    Deutsche Übersetzung der Patentansprüche nach Artikel 67 (1) EPÜ
    Patentansprüche
    1. Verfahren zur Bestimmung der Karzinogenizität von chemischen oder physikalischen Agentien, dadurch gekennzeichnet, daß man eine ausgewählte Linie von mit Tumorvirus transformierten Zellen dem zu prüfenden Agens aussetzt und bestimmt, ob diese Aussetzung endogene Replikation der Virus DNS in diesen Zellen induziert, wobei die Induktion durch in situ Hybridisierung die Karzinogenizität des geprüften Agens anzeigt.
    2c Verfahren nach Anspruch 1, wobei die verwendeten Zellen SV40-transformierte Zellen des Chinesischen Hamsters sind.
    3. Verfahren nach Anspruch 2, wobei die verwendete ZeIllinie ein von Embryozellen des Chinesischen Hamsters abgeleitetes, als CO50 bezeichnetes Klon-Isolat oder eine als CO60 bezeichnete Zellinie oder eine andere SV40 transformierte Zellinie des Chinesischen Hamsters
    ist.
    35
    Verfahren nach einem der Ansprüche 1 bis 3, wobei die Replication der Virus- DNS durch die _in situ Hybridisierüngstechnik bestimmt wird,bei der der durch die Behandlung mit Karzinogenen erhöhte Gehalt an SV40-DNS oder an anderen spezifischen DNS-
    32
    Sequenzen durch Hybridisierung gegen eine P SV40-
    32 DNS Probe oder eine andere spezifische P-DNS Probe bestimmt wird.
    5. Verfahren nach einem der Ansprüche 1 bis 4, wobei das Karzinogen ein chemisches Karzinogen ist.
    6. Verfahren nach einem der Ansprüche 1 bis 4, wobei das Karzinogen ein physikalisches Karzinogen ist.
    7. Verfahren nach einem der Ansprüche 1 bis 6, dadurch gekennzeichnet, daß man das Ausmaß der DNS-Vermehrung mißt,das Information über den Grad der Karzinogenizität des geprüften Agens liefert.
    8. Verfahren zur Früherkennung von Karzinogenese auf
    der Basis von Genvermehrung,gekennzeichnet durch in situ Hybridisierung zwischen denauf Karzinogenizität geprüften
    32
    Zellen und P DNS Proben für vermehrte DNS Sequen-
    5 ζen, wobei der Test mit dispergierten Zellen(dispersed cell
    assay) oder der Test mit Zellen auf einer Scheibe (disc cell assay) benutzt wird.
    9. Testsatz zur Ausführung des Verfahrens gemäß Ansprüche 1 bis 8,enthaltend in Kombination Testzellen von C05Q, CO60 oder ähnliche Zellinien, foetales Kälberse-
    32 rum, Filter, Denhardt-Lösung, P SV40 DNA, markiert
    Q " O
    mit etwa 10 Impulsen/Minute pro Testsatz bei 10 Impulsen/Minute/^g DNS und durch mit Behandlung mit
    Ultraschall denaturierte Lachssperma-DNS. 35
    «3
    1 10. SV40 transformierte Embryozellinie C050 des Chinesischen Hamsters C.N.C.M. (Institut Pasteur) Nr. 1-188.
    11..-SV40 transformierte Embryozellinie C060 des Chinesi-5 sehen Hamsters C.N.C.M. (Institut Pasteur) Nr. 1-189.
    12. Verwendung der Zellinien nach einem der Ansprüche und 11 zur Bestimmung der Karzinogenizität von chemischen oder physikalischen Agentien. 10
DE198282102458T 1981-03-25 1982-03-24 Verfahren zur feststellung der carcinogenen aktivitaet von chemischen oder physikalischen mitteln. Pending DE62237T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IL62479A IL62479A (en) 1981-03-25 1981-03-25 Assay for the determination of carcinogenicity

Publications (1)

Publication Number Publication Date
DE62237T1 true DE62237T1 (de) 1983-04-28

Family

ID=11052533

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198282102458T Pending DE62237T1 (de) 1981-03-25 1982-03-24 Verfahren zur feststellung der carcinogenen aktivitaet von chemischen oder physikalischen mitteln.
DE8282102458T Expired DE3264849D1 (en) 1981-03-25 1982-03-24 Process for the determination of the carcinogenicity of chemical or physical agents

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8282102458T Expired DE3264849D1 (en) 1981-03-25 1982-03-24 Process for the determination of the carcinogenicity of chemical or physical agents

Country Status (5)

Country Link
US (1) US4532220A (de)
EP (1) EP0062237B1 (de)
JP (1) JPS57173756A (de)
DE (2) DE62237T1 (de)
IL (1) IL62479A (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2535342B1 (fr) * 1982-11-02 1986-05-16 Maillet Jeanne Extrait de mycobacterie en tant que nouveau moyen de detection d'activites cancerigenes et procede mettant en oeuvre ce moyen
US4689295A (en) * 1983-01-20 1987-08-25 Integrated Genetics, Inc. Test for Salmonella
US5030565A (en) * 1983-08-17 1991-07-09 Scripps Clinic And Research Foundation Polypeptide-induced monoclonal receptors to protein ligands
US5733738A (en) * 1983-08-17 1998-03-31 Ligand Pharmaceuticals Polypeptide-induced monoclonal receptors to protein ligands
US4792520A (en) * 1984-02-16 1988-12-20 University Of Cincinnati Methods and kits for identifying mutagenic agents and molecular mutations in DNA in mammalian cells
US4675283A (en) * 1984-07-19 1987-06-23 Massachusetts Institute Of Technology Detection and isolation of homologous, repeated and amplified nucleic acid sequences
US4735895A (en) * 1984-12-28 1988-04-05 Oncotech, Inc. Cancer susceptibility test
US5258283A (en) * 1985-11-05 1993-11-02 Battelle Memorial Institute Detection and differentiation of coxiella burnetii in biological fluids
US4876186A (en) * 1985-11-05 1989-10-24 Battelle Development Corporation Detection and differentiation of coxiella burnetii in biological fluids
WO1989005864A1 (en) * 1987-12-15 1989-06-29 The Trustees Of Princeton University Transgenic testing systems for mutagens and carcinogens
GB8905394D0 (en) * 1989-03-09 1989-04-19 Delta Biotechnology Ltd Detection of certain molecules
US5681737A (en) * 1993-10-15 1997-10-28 The Regents Of The University Of California Detection system for mutagens that also identifies mutagenic changes
US5501954A (en) * 1994-06-13 1996-03-26 Genzyme Corporation Method of detecting cellular material
JP2011002417A (ja) 2009-06-22 2011-01-06 Jx Nippon Oil & Energy Corp 絶縁抵抗測定装置及び絶縁抵抗測定方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4066510A (en) * 1976-05-24 1978-01-03 Massachusetts Institute Of Technology Assay for mutagenesis in diploid human lymphoblasts
US4072574A (en) * 1976-07-30 1978-02-07 The Institute For Cancer Research System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents
FR2403557A1 (fr) * 1977-09-19 1979-04-13 Beljanski Mirko Procede et reactifs de detection des substances cancerigenes et des substances anticancereuses
US4299915A (en) * 1977-10-17 1981-11-10 Massachusetts Institute Of Technology Assay for mutagenesis in bacterial cells
US4352880A (en) * 1979-09-17 1982-10-05 Massachusetts Institute Of Technology Diffusion bioassay for the quantitative determination of mutagenicity
US4302535A (en) * 1979-09-27 1981-11-24 Massachusetts Institute Of Technology Assay for mutagenesis in heterozygous diploid human lymphoblasts
US4345026A (en) * 1981-01-12 1982-08-17 The Children's Hospital Medical Center Mutagenicity assay

Also Published As

Publication number Publication date
US4532220A (en) 1985-07-30
IL62479A (en) 1984-11-30
EP0062237B1 (de) 1985-07-24
JPS57173756A (en) 1982-10-26
EP0062237A1 (de) 1982-10-13
DE3264849D1 (en) 1985-08-29
IL62479A0 (en) 1981-05-20

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