DE60336736D1 - Codon-optimierte synthetische plasmide - Google Patents

Codon-optimierte synthetische plasmide

Info

Publication number
DE60336736D1
DE60336736D1 DE60336736T DE60336736T DE60336736D1 DE 60336736 D1 DE60336736 D1 DE 60336736D1 DE 60336736 T DE60336736 T DE 60336736T DE 60336736 T DE60336736 T DE 60336736T DE 60336736 D1 DE60336736 D1 DE 60336736D1
Authority
DE
Germany
Prior art keywords
plasmide
codon
optimized synthetic
optimized
synthetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60336736T
Other languages
English (en)
Inventor
Ruxandra Draghia-Akli
Ronald V Abruzzese
Douglas R Kern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VGX Pharmaceuticals LLC
Original Assignee
VGX Pharmaceuticals LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VGX Pharmaceuticals LLC filed Critical VGX Pharmaceuticals LLC
Application granted granted Critical
Publication of DE60336736D1 publication Critical patent/DE60336736D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/02—Drugs for disorders of the endocrine system of the hypothalamic hormones, e.g. TRH, GnRH, CRH, GRH, somatostatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth-hormone releasing factors (GH-RF) (Somatoliberin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
DE60336736T 2002-07-16 2003-07-15 Codon-optimierte synthetische plasmide Expired - Lifetime DE60336736D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39624702P 2002-07-16 2002-07-16
PCT/US2003/021917 WO2004007678A2 (en) 2002-07-16 2003-07-15 Codon optimized synthetic plasmids

Publications (1)

Publication Number Publication Date
DE60336736D1 true DE60336736D1 (de) 2011-05-26

Family

ID=30115994

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60336736T Expired - Lifetime DE60336736D1 (de) 2002-07-16 2003-07-15 Codon-optimierte synthetische plasmide

Country Status (8)

Country Link
US (2) US7316925B2 (de)
EP (1) EP1543020B1 (de)
AR (1) AR040575A1 (de)
AT (1) ATE505476T1 (de)
AU (1) AU2003249208B2 (de)
DE (1) DE60336736D1 (de)
TW (1) TW200403338A (de)
WO (1) WO2004007678A2 (de)

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EP2842964A1 (de) * 2001-06-05 2015-03-04 Curevac GmbH Virtuelles Verfahren zur Ermittlung einer modifzierten mRNA-Sequenz
DE10162480A1 (de) 2001-12-19 2003-08-07 Ingmar Hoerr Die Applikation von mRNA für den Einsatz als Therapeutikum gegen Tumorerkrankungen
EP1539959A2 (de) * 2002-09-18 2005-06-15 Centre Hospitalier de l'Université de Montréal (CHUM) Ghrh-analoge
US7361642B2 (en) 2003-08-04 2008-04-22 Vgx Pharmaceuticals, Inc. Canine specific growth hormone releasing hormone
US7825233B2 (en) * 2004-06-30 2010-11-02 Allergan, Inc. Optimizing expression of active Botulinum Toxin type E
JP4550115B2 (ja) * 2004-08-03 2010-09-22 ジーンアート・アクチエンゲゼルシャフト ＣｐＧ含量を変化させることにより遺伝子発現を調整する方法
CA2575994A1 (en) * 2004-08-04 2006-02-16 Allergan, Inc. Optimizing expression of active botulinum toxin type a
DE102004042546A1 (de) * 2004-09-02 2006-03-09 Curevac Gmbh Kombinationstherapie zur Immunstimulation
KR101136688B1 (ko) * 2004-09-09 2012-04-18 퀄컴 인코포레이티드 이방성 필터링을 이용한 싱글-패스 리샘플링 시스템 및방법
US7846720B2 (en) * 2005-01-26 2010-12-07 Vgx Pharmaceuticals, Inc. Optimized high yield synthetic plasmids
WO2009022236A2 (en) 2007-08-16 2009-02-19 Tripep Ab Immunogen platform
CN201397956Y (zh) * 2009-03-23 2010-02-03 富士康(昆山)电脑接插件有限公司 电连接器组件
NZ598275A (en) * 2009-08-06 2014-11-28 Cmc Icos Biolog Inc Methods for improving recombinant protein expression
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
DK3590949T3 (da) 2010-10-01 2022-07-11 Modernatx Inc Ribonukleinsyrer indeholdende n1-methyl-pseudouracil og anvendelse heraf
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
SG10201602654SA (en) 2011-10-03 2016-05-30 Moderna Therapeutics Inc Modified nucleosides,nucleotides,and nucleic acids,and uses thereof
US8993316B2 (en) 2011-11-16 2015-03-31 Brian P. Hanley Methods and compositions for gene therapy and GHRH therapy
WO2013090648A1 (en) 2011-12-16 2013-06-20 modeRNA Therapeutics Modified nucleoside, nucleotide, and nucleic acid compositions
US9347073B2 (en) 2012-02-10 2016-05-24 The Board Of Trustees Of The Leland Stanford Junior University Mini-intronic plasmid vectors
US9303079B2 (en) 2012-04-02 2016-04-05 Moderna Therapeutics, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
JP2015516143A (ja) 2012-04-02 2015-06-08 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． ヒト疾患に関連するタンパク質の産生のための修飾ポリヌクレオチド
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
LT2922554T (lt) 2012-11-26 2022-06-27 Modernatx, Inc. Terminaliai modifikuota rnr
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
AU2014329452B2 (en) 2013-10-03 2019-06-20 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
WO2015062738A1 (en) 2013-11-01 2015-05-07 Curevac Gmbh Modified rna with decreased immunostimulatory properties
CN106659803A (zh) 2014-04-23 2017-05-10 摩登纳特斯有限公司 核酸疫苗
WO2020014528A1 (en) * 2018-07-13 2020-01-16 The Regents Of The University Of California Retrotransposon-based delivery vehicle and methods of use thereof
US11795207B2 (en) 2021-03-30 2023-10-24 AAVnerGene Inc. Modified plasma clotting factor VIII and method of use thereof
JP2024511851A (ja) 2021-03-30 2024-03-15 エーエーブイネルジーン インク． 改変血漿凝固第ｖｉｉｉ因子およびその使用方法
US20230365991A1 (en) 2022-05-12 2023-11-16 AAVnerGene Inc. Compositions and methods for recombinant parvovirus production

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US5134120A (en) * 1984-08-03 1992-07-28 Cornell Research Foundation, Inc. Use of growth hormone to enhance porcine weight gain
FR2622455B1 (fr) * 1987-11-04 1991-07-12 Agronomique Inst Nat Rech Application du facteur de stimulation de la secretion de l'hormone de croissance humaine, de ses fragments actifs et des analogues correspondants, pour augmenter la production laitiere et le poids des nouveau-nes chez les mammiferes
US5298422A (en) * 1991-11-06 1994-03-29 Baylor College Of Medicine Myogenic vector systems
JP2586367B2 (ja) * 1992-04-15 1997-02-26 日本電気株式会社 軟磁性材料とその製造方法および磁気ヘッド
US6114148C1 (en) * 1996-09-20 2012-05-01 Gen Hospital Corp High level expression of proteins
WO1999005300A2 (en) * 1997-07-24 1999-02-04 Valentis, Inc. Ghrh expression system and methods of use
US6924365B1 (en) * 1998-09-29 2005-08-02 Transkaryotic Therapies, Inc. Optimized messenger RNA
BR0012748A (pt) * 1999-07-26 2003-06-10 Baylor College Of Medcine Análogo super-ativo do hormÈnio de lberação do hormõnio do crescimento porcino
AU2001245427A1 (en) * 2000-03-03 2001-09-17 Valentis, Inc. Nucleic acid formulations for gene delivery and methods of use
AR035671A1 (es) * 2000-12-12 2004-06-23 Advisys Inc Administracion de secuencia de acido nucleico a un animal hembra, para aumentar el crecimiento de la descendencia

Also Published As

Publication number Publication date
US20080194511A1 (en) 2008-08-14
US20040092009A1 (en) 2004-05-13
WO2004007678A3 (en) 2004-08-12
US7316925B2 (en) 2008-01-08
WO2004007678B1 (en) 2004-10-07
AR040575A1 (es) 2005-04-13
EP1543020B1 (de) 2011-04-13
EP1543020A2 (de) 2005-06-22
WO2004007678A2 (en) 2004-01-22
AU2003249208B2 (en) 2010-03-04
EP1543020A4 (de) 2006-11-22
ATE505476T1 (de) 2011-04-15
AU2003249208A1 (en) 2004-02-02
TW200403338A (en) 2004-03-01

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