DE60335129D1 - VERFAHREN ZUR ERZEUGUNG VERBESSERTER ANTIKöRPER PRODUZIERENDER ZELLINIEN MIT VERBESSERTEN WACHSTUMSEIGENSCHAFTEN - Google Patents

VERFAHREN ZUR ERZEUGUNG VERBESSERTER ANTIKöRPER PRODUZIERENDER ZELLINIEN MIT VERBESSERTEN WACHSTUMSEIGENSCHAFTEN

Info

Publication number
DE60335129D1
DE60335129D1 DE60335129T DE60335129T DE60335129D1 DE 60335129 D1 DE60335129 D1 DE 60335129D1 DE 60335129 T DE60335129 T DE 60335129T DE 60335129 T DE60335129 T DE 60335129T DE 60335129 D1 DE60335129 D1 DE 60335129D1
Authority
DE
Germany
Prior art keywords
genes
protein production
production
useful
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60335129T
Other languages
English (en)
Inventor
Luigi Grasso
Bradford J Kline
Nicholas C Nicolaides
Philip M Sass
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Morphotek Inc
Original Assignee
Morphotek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphotek Inc filed Critical Morphotek Inc
Application granted granted Critical
Publication of DE60335129D1 publication Critical patent/DE60335129D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DE60335129T 2002-07-19 2003-07-21 VERFAHREN ZUR ERZEUGUNG VERBESSERTER ANTIKöRPER PRODUZIERENDER ZELLINIEN MIT VERBESSERTEN WACHSTUMSEIGENSCHAFTEN Expired - Lifetime DE60335129D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39702702P 2002-07-19 2002-07-19
PCT/US2003/022743 WO2004009782A2 (en) 2002-07-19 2003-07-21 Methods for generating enhanced antibody-producing cell lines with improved growth characteristics

Publications (1)

Publication Number Publication Date
DE60335129D1 true DE60335129D1 (de) 2011-01-05

Family

ID=30770977

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60335129T Expired - Lifetime DE60335129D1 (de) 2002-07-19 2003-07-21 VERFAHREN ZUR ERZEUGUNG VERBESSERTER ANTIKöRPER PRODUZIERENDER ZELLINIEN MIT VERBESSERTEN WACHSTUMSEIGENSCHAFTEN

Country Status (8)

Country Link
US (2) US20040115695A1 (de)
EP (1) EP1551988B1 (de)
JP (1) JP4527531B2 (de)
AT (1) ATE489474T1 (de)
AU (1) AU2003256642B2 (de)
CA (1) CA2493679A1 (de)
DE (1) DE60335129D1 (de)
WO (1) WO2004009782A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60329526D1 (de) 2002-11-15 2009-11-12 Morphotek Inc Verfahren zur erzeugung einer hohen antikörperproduktion von durch in-vitro-immunisierung entstandenen hybridomen
WO2005080431A2 (en) * 2004-02-12 2005-09-01 Morphotek, Inc. Monoclonal antibodies that specifically bind to folate receptor alpha
WO2006005367A1 (en) 2004-07-14 2006-01-19 Igeneon Krebs-Immuntherapie Forschungs-Und Entwicklungs-Ag N-glycosylated antibody
AU2006223301B2 (en) 2005-03-10 2010-11-04 Eisai, Inc. Anti-mesothelin antibodies
CA2607444C (en) 2005-04-22 2015-03-10 Morphotek, Inc. Antibodies with immune effector activity and that internalize in folate receptor alpha-positive cells
AU2007237985A1 (en) * 2006-04-17 2007-10-25 Morphotek, Inc. Whole genome evolution technology applied to improve protein and antibody yields by cells
EP2115127A4 (de) * 2007-01-17 2010-04-07 Life Technologies Corp Verfahren und zusammensetzungen zur verbesserung der zellgesundheit in kulturen
WO2008103474A1 (en) 2007-02-20 2008-08-28 Anaptysbio, Inc. Methods of generating libraries and uses thereof
EP2855506B1 (de) * 2012-05-25 2017-03-08 Well Resources Limited Peptid und verwendung davon

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4732973A (en) * 1984-06-14 1988-03-22 Chiron Corporation Active site modified protease α-1-antitrypsin inhibitors
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein
US5530101A (en) * 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6191268B1 (en) * 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
US5907079A (en) * 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US5885827A (en) * 1996-01-23 1999-03-23 The Regents Of The Universtiy Of California Eukaryotic high rate mutagenesis system
US6326204B1 (en) * 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP2261373A3 (de) * 1997-01-17 2011-12-14 Codexis Mayflower Holdings, LLC Evolution prokaryotischer ganzer Zellen durch rekursive Sequenzrekombination
US20020012991A1 (en) * 1997-04-07 2002-01-31 Florence Chua Nee Ho Kit Fong Cell culture media for enhanced protein production
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
US6890730B1 (en) * 1999-12-10 2005-05-10 The Salk Institute Sequence and method for increasing protein expression in cellular expression systems
US6825038B2 (en) * 2000-05-12 2004-11-30 The Johns Hopkins University Method for generating hypermutable organisms
CA2428214C (en) * 2000-11-07 2010-02-02 Morphotek, Inc. Methods for generating genetically altered antibody-producing cell lines with improved antibody characteristics
JP5036119B2 (ja) * 2001-01-15 2012-09-26 モーフオテク・インコーポレーテツド ミスマッチ修復の化学阻害剤

Also Published As

Publication number Publication date
WO2004009782A2 (en) 2004-01-29
AU2003256642A2 (en) 2004-02-09
WO2004009782A3 (en) 2004-09-23
US20040115695A1 (en) 2004-06-17
EP1551988B1 (de) 2010-11-24
AU2003256642A1 (en) 2004-02-09
US20090155797A1 (en) 2009-06-18
JP2005533499A (ja) 2005-11-10
JP4527531B2 (ja) 2010-08-18
CA2493679A1 (en) 2004-01-29
ATE489474T1 (de) 2010-12-15
EP1551988A2 (de) 2005-07-13
AU2003256642B2 (en) 2008-05-01
EP1551988A4 (de) 2007-11-14

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