DE60323631D1 - Verfahren zur reinigung einer rhizomucor aspartat-protease - Google Patents

Verfahren zur reinigung einer rhizomucor aspartat-protease

Info

Publication number
DE60323631D1
DE60323631D1 DE60323631T DE60323631T DE60323631D1 DE 60323631 D1 DE60323631 D1 DE 60323631D1 DE 60323631 T DE60323631 T DE 60323631T DE 60323631 T DE60323631 T DE 60323631T DE 60323631 D1 DE60323631 D1 DE 60323631D1
Authority
DE
Germany
Prior art keywords
microbial protease
separation medium
rhizomucor
cleaning
aspartate protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60323631T
Other languages
English (en)
Inventor
Van Rudolf Beckhoven
Thierry Jean-Bernard Naeye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Application granted granted Critical
Publication of DE60323631D1 publication Critical patent/DE60323631D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
DE60323631T 2002-05-29 2003-05-28 Verfahren zur reinigung einer rhizomucor aspartat-protease Expired - Lifetime DE60323631D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02100578 2002-05-29
PCT/EP2003/005724 WO2003100048A1 (en) 2002-05-29 2003-05-28 Method for the purification of microbial protease

Publications (1)

Publication Number Publication Date
DE60323631D1 true DE60323631D1 (de) 2008-10-30

Family

ID=29558402

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60323631T Expired - Lifetime DE60323631D1 (de) 2002-05-29 2003-05-28 Verfahren zur reinigung einer rhizomucor aspartat-protease

Country Status (7)

Country Link
US (1) US7670821B2 (de)
EP (1) EP1507858B1 (de)
AT (1) ATE408681T1 (de)
AU (1) AU2003232845A1 (de)
DE (1) DE60323631D1 (de)
DK (1) DK1507858T3 (de)
WO (1) WO2003100048A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0004808D0 (sv) * 2000-12-20 2000-12-20 Apbiotech Ab Method for the purification of an enzyme
US20070215548A1 (en) * 2006-01-05 2007-09-20 Zhou Joe Xin H Systems and methods for packing chromatography columns
WO2007118838A1 (en) 2006-04-13 2007-10-25 Dsm Ip Assets B.V. Liquid composition comprising an aspartic protease
CN102383555A (zh) * 2011-10-11 2012-03-21 常熟市华夏建筑节能材料有限公司 一种节能天花板
KR101743094B1 (ko) * 2015-05-13 2017-06-07 대한민국 신규 리조무코르 푸실루스 ｍ2559 균주 및 이의 용도
WO2017089613A1 (en) 2015-11-26 2017-06-01 Csk Food Enrichment B.V. Liquid composition comprising an aspartic protease having improved enzyme stability

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE280333C (de)
US2683682A (en) * 1950-02-06 1954-07-13 Research Corp Differential inactivation of enzymes
DK110031A (de) * 1965-12-02
US4086139A (en) * 1976-04-09 1978-04-25 Gb Fermentation Industries Inc. Differential inactivation of amylase in amylase-protease mixtures
US4721673A (en) * 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
US4743551A (en) 1984-11-05 1988-05-10 Miles Inc. Purification of microbial rennet from Mucor miehei
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
DD280333A1 (de) * 1989-03-02 1990-07-04 Adw Ddr Verfahren zur gewinnung von milchfaellenden enzymen
US5139943A (en) * 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
DK165090D0 (da) 1990-07-09 1990-07-09 Kem En Tec As Konglomererede partikler
SE9101149D0 (sv) 1991-04-17 1991-04-17 Pharmacia Lkb Biotech Beads for down stream processing
AU653279B2 (en) * 1991-12-30 1994-09-22 Sanofi Novel 2-saccharinylmethyl heterocyclic carboxylates useful as proteolytic enzyme inhibitors and compositions and method of use thereof
DE4323913A1 (de) 1993-04-03 1995-01-19 Merck Patent Gmbh Trennmaterialien für die hydrophobe Chromatographie
DK47493D0 (da) 1993-04-27 1993-04-27 Novo Nordisk As Fremgangsmaade til fremstilling af ost
US20040214157A1 (en) 1994-06-29 2004-10-28 Simon C. Burton Chromatographic resins and methods for using same
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
FR2737892B1 (fr) * 1995-08-17 1997-10-24 Fournier Ind & Sante Nouveaux composes de benzenesulfonamide, procede de preparation et utilisation en therapeutique
EP0865485A1 (de) 1995-12-07 1998-09-23 Novo Nordisk A/S Selektive inaktivierung von enzymaktivitäten
SE9700383D0 (sv) 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
US6531485B2 (en) * 1997-06-23 2003-03-11 Pfizer Inc. Prostaglandin agonists
AU2001231527A1 (en) * 2000-02-08 2001-08-20 Upfront Chromatography A/S Novel resin materials and their use for recovering proteins or peptides
SE0004808D0 (sv) 2000-12-20 2000-12-20 Apbiotech Ab Method for the purification of an enzyme

Also Published As

Publication number Publication date
WO2003100048A1 (en) 2003-12-04
AU2003232845A1 (en) 2003-12-12
US7670821B2 (en) 2010-03-02
ATE408681T1 (de) 2008-10-15
EP1507858B1 (de) 2008-09-17
DK1507858T3 (da) 2009-02-02
US20050153418A1 (en) 2005-07-14
EP1507858A1 (de) 2005-02-23

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Legal Events

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