DE60317693D1 - Methode und verwendung von thermostabilen rna-ligasen - Google Patents

Methode und verwendung von thermostabilen rna-ligasen

Info

Publication number
DE60317693D1
DE60317693D1 DE60317693T DE60317693T DE60317693D1 DE 60317693 D1 DE60317693 D1 DE 60317693D1 DE 60317693 T DE60317693 T DE 60317693T DE 60317693 T DE60317693 T DE 60317693T DE 60317693 D1 DE60317693 D1 DE 60317693D1
Authority
DE
Germany
Prior art keywords
thermostabiles
rna ligases
ligases
rna
thermostabiles rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60317693T
Other languages
English (en)
Other versions
DE60317693T2 (de
Inventor
Arnthor Aevarsson
Olafur H Fridjonsson
Thorarinn Blondal
Sigurlaug Skirnisdottir
Sigridur Hjorleifsdottir
Gudmundur Oli Hreggvidsson
Jakob Kristjansson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epicentre Technologies Corp
Original Assignee
Prokaria Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ltd filed Critical Prokaria Ltd
Publication of DE60317693D1 publication Critical patent/DE60317693D1/de
Application granted granted Critical
Publication of DE60317693T2 publication Critical patent/DE60317693T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE60317693T 2002-09-20 2003-09-19 Methode und verwendung von thermostabilen rna-ligasen Expired - Lifetime DE60317693T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US251667 1988-09-29
US10/251,667 US20040058330A1 (en) 2002-09-20 2002-09-20 Methods of use for thermostable RNA ligases
PCT/IS2003/000029 WO2004027056A2 (en) 2002-09-20 2003-09-19 Methods of use for thermostable rna ligases

Publications (2)

Publication Number Publication Date
DE60317693D1 true DE60317693D1 (de) 2008-01-03
DE60317693T2 DE60317693T2 (de) 2008-10-30

Family

ID=31992794

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60317693T Expired - Lifetime DE60317693T2 (de) 2002-09-20 2003-09-19 Methode und verwendung von thermostabilen rna-ligasen

Country Status (5)

Country Link
US (1) US20040058330A1 (de)
EP (1) EP1546355B1 (de)
AU (1) AU2003260945A1 (de)
DE (1) DE60317693T2 (de)
WO (1) WO2004027056A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1791682B (zh) * 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
WO2005047543A2 (en) * 2003-06-10 2005-05-26 Applera Corporation Ligation assay
US20090246756A1 (en) * 2004-05-06 2009-10-01 Prokaria Ehe Thermostable polypeptide having polynucleotide kinase activity and/or phosphatase activity
US20070092883A1 (en) * 2005-10-26 2007-04-26 De Luwe Hoek Octrooien B.V. Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA)
US20080194416A1 (en) * 2007-02-08 2008-08-14 Sigma Aldrich Detection of mature small rna molecules
WO2009135212A2 (en) * 2008-05-02 2009-11-05 Epicentre Technologies Corporation Selective 5' ligation tagging of rna
US20090061424A1 (en) * 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
JP2012517813A (ja) * 2009-02-16 2012-08-09 エピセンター テクノロジーズ コーポレイション １本鎖ｄｎａの鋳型非依存性ライゲーション
US20130143276A1 (en) * 2010-04-01 2013-06-06 New England Biolabs, Inc. Compositions and Methods for Adenylating Oligonucleotides
EP2665833B1 (de) 2011-01-17 2017-04-19 Life Technologies Corporation Arbeitsablauf zur nachweis von liganden mit nukleinsäuren
US20190062827A1 (en) 2016-03-14 2019-02-28 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
CA3158739A1 (en) * 2019-11-19 2021-05-27 Xiao Bing Wang Method for identifying gene fusions by circle cdna amplification
CN111334501B (zh) * 2020-03-13 2024-03-15 北京安诺优达医学检验实验室有限公司 适于冠状病毒保存和稀释复合缓冲液及其应用
WO2022015461A1 (en) 2020-07-17 2022-01-20 Qiagen Beverly Llc Thermostable ligase with reduced sequence bias
CN112680797B (zh) * 2021-02-04 2023-09-26 广州大学 一种去除高丰度rna的测序文库及其构建方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) * 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US6413720B1 (en) * 1999-01-11 2002-07-02 Signal Pharmaceuticals, Inc. Method for the affinity isolation of newly synthesized RNA
US6492161B1 (en) * 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US6242189B1 (en) * 1999-10-01 2001-06-05 The Regents Of The University Of California Selective isolation of bacterial mRNA
US20010031466A1 (en) * 1999-12-02 2001-10-18 Malek Lawrence T. Preparation of sequence libraries from non-denatured RNA and kits therefor
US7229760B2 (en) * 2000-03-24 2007-06-12 Micromet Ag mRNA amplification
US6368801B1 (en) * 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase

Also Published As

Publication number Publication date
EP1546355A2 (de) 2005-06-29
US20040058330A1 (en) 2004-03-25
DE60317693T2 (de) 2008-10-30
AU2003260945A1 (en) 2004-04-08
WO2004027056A3 (en) 2004-04-22
EP1546355B1 (de) 2007-11-21
WO2004027056A2 (en) 2004-04-01
AU2003260945A8 (en) 2004-04-08

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: EPICENTRE TECHNOLOGIES CORP., MADISON, WIS., US