DE60314491D1 - Hefe-Extraktlösung, ihre Herstellung und Verwendung zur zellfreien Proteinsynthese - Google Patents

Hefe-Extraktlösung, ihre Herstellung und Verwendung zur zellfreien Proteinsynthese

Info

Publication number
DE60314491D1
DE60314491D1 DE60314491T DE60314491T DE60314491D1 DE 60314491 D1 DE60314491 D1 DE 60314491D1 DE 60314491 T DE60314491 T DE 60314491T DE 60314491 T DE60314491 T DE 60314491T DE 60314491 D1 DE60314491 D1 DE 60314491D1
Authority
DE
Germany
Prior art keywords
cell
protein synthesis
free protein
extract solution
yeast extract
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60314491T
Other languages
English (en)
Other versions
DE60314491T2 (de
Inventor
Takashi Suzuki
Toru Ezure
Masaaki Ito
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Application granted granted Critical
Publication of DE60314491D1 publication Critical patent/DE60314491D1/de
Publication of DE60314491T2 publication Critical patent/DE60314491T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04M—TELEPHONIC COMMUNICATION
    • H04M1/00—Substation equipment, e.g. for use by subscribers
    • H04M1/60—Substation equipment, e.g. for use by subscribers including speech amplifiers
    • H04M1/6033—Substation equipment, e.g. for use by subscribers including speech amplifiers for providing handsfree use or a loudspeaker mode in telephone sets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04R—LOUDSPEAKERS, MICROPHONES, GRAMOPHONE PICK-UPS OR LIKE ACOUSTIC ELECTROMECHANICAL TRANSDUCERS; DEAF-AID SETS; PUBLIC ADDRESS SYSTEMS
    • H04R1/00—Details of transducers, loudspeakers or microphones
    • H04R1/10—Earpieces; Attachments therefor ; Earphones; Monophonic headphones
    • H04R1/1041—Mechanical or electronic switches, or control elements
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04R—LOUDSPEAKERS, MICROPHONES, GRAMOPHONE PICK-UPS OR LIKE ACOUSTIC ELECTROMECHANICAL TRANSDUCERS; DEAF-AID SETS; PUBLIC ADDRESS SYSTEMS
    • H04R2499/00—Aspects covered by H04R or H04S not otherwise provided for in their subgroups
    • H04R2499/10—General applications
    • H04R2499/11—Transducers incorporated or for use in hand-held devices, e.g. mobile phones, PDA's, camera's
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04R—LOUDSPEAKERS, MICROPHONES, GRAMOPHONE PICK-UPS OR LIKE ACOUSTIC ELECTROMECHANICAL TRANSDUCERS; DEAF-AID SETS; PUBLIC ADDRESS SYSTEMS
    • H04R3/00—Circuits for transducers, loudspeakers or microphones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Signal Processing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE60314491T 2003-01-07 2003-12-30 Hefe-Extraktlösung, ihre Herstellung und Verwendung zur zellfreien Proteinsynthese Expired - Fee Related DE60314491T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003001317 2003-01-07
JP2003001317A JP2004208640A (ja) 2003-01-07 2003-01-07 無細胞系タンパク質合成用酵母抽出液およびその調製方法、ならびにそれを用いた無細胞系タンパク質合成方法

Publications (2)

Publication Number Publication Date
DE60314491D1 true DE60314491D1 (de) 2007-08-02
DE60314491T2 DE60314491T2 (de) 2008-02-21

Family

ID=32501191

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60314491T Expired - Fee Related DE60314491T2 (de) 2003-01-07 2003-12-30 Hefe-Extraktlösung, ihre Herstellung und Verwendung zur zellfreien Proteinsynthese

Country Status (7)

Country Link
US (1) US7387884B2 (de)
EP (1) EP1437399B1 (de)
JP (1) JP2004208640A (de)
KR (1) KR20040063809A (de)
CN (1) CN1530373A (de)
AT (1) ATE365203T1 (de)
DE (1) DE60314491T2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4091901B2 (ja) * 2003-11-13 2008-05-28 株式会社島津製作所 無細胞系タンパク質合成におけるミクロソーム膜添加による翻訳後修飾方法
WO2014144583A2 (en) 2013-03-15 2014-09-18 Northwestern University Methods for cell- free protein synthesis
CN106459892A (zh) 2014-04-24 2017-02-22 快速微型生物系统公司 微生物生长培养基和使用其的方法
CN106978349B (zh) * 2016-09-30 2018-06-22 康码（上海）生物科技有限公司 一种体外蛋白质合成的试剂盒及其制备方法
CN108535489B (zh) * 2017-03-04 2019-04-19 康码（上海）生物科技有限公司 一种用于体外蛋白质合成的蛋白合成体系、试剂盒及其制备方法
JP2020127364A (ja) * 2017-04-28 2020-08-27 Ｓｐｉｂｅｒ株式会社 無細胞タンパク質合成用反応混合物、これを用いた無細胞タンパク質合成方法、及び無細胞タンパク質合成用キット
CN107299058A (zh) * 2017-07-21 2017-10-27 天津大学 一种用于无细胞蛋白质合成的细胞提取物及其制备方法
CN109321620A (zh) * 2017-07-31 2019-02-12 康码（上海）生物科技有限公司 一种蛋白合成冻干制剂及其制备方法和应用
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
US12098433B2 (en) 2018-08-03 2024-09-24 Northwestern University On demand, portable, cell-free molecular sensing platform
CN109321547B (zh) * 2018-10-08 2021-01-15 广东菲鹏生物有限公司 一种RNasin的制备方法
EP3670646A1 (de) * 2018-12-21 2020-06-24 Ohly GmbH Funktionelles hefeproteinkonzentrat
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69940525D1 (de) * 1999-05-11 2009-04-16 Cellfree Sciences Co Ltd Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins
JP2002125693A (ja) * 2000-10-19 2002-05-08 Toyobo Co Ltd 無細胞タンパク質合成用細胞抽出液組成物
JP4750299B2 (ja) * 2001-03-08 2011-08-17 独立行政法人理化学研究所 無細胞タンパク質合成系によるタンパク質の製造方法

Also Published As

Publication number Publication date
CN1530373A (zh) 2004-09-22
US7387884B2 (en) 2008-06-17
EP1437399B1 (de) 2007-06-20
DE60314491T2 (de) 2008-02-21
EP1437399A1 (de) 2004-07-14
US20040137560A1 (en) 2004-07-15
KR20040063809A (ko) 2004-07-14
ATE365203T1 (de) 2007-07-15
JP2004208640A (ja) 2004-07-29

Similar Documents

Publication Publication Date Title
DE60314491D1 (de) Hefe-Extraktlösung, ihre Herstellung und Verwendung zur zellfreien Proteinsynthese
EP2270031A3 (de) Heterologe Expression neisserscher Proteine
BR0210599A (pt) Uso de monóxido de carbono, método para manutenção de uma célula animal in vitro, artigo de manufatura, bem como meio celular estéril
WO2004029219A3 (en) Cell-based rna interference and related methods and compositions
WO2002067812A3 (en) Tympanic membrane patch
WO2001080865A3 (en) Joint repair using mesenchymal stem cells
AU2002320317A1 (en) Novel mesogens and methods for their synthesis and use
AU2003295310A1 (en) Battery-boosted, rapid startup of frozen fuel cell
WO2006045116A3 (en) In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
NO20052363L (no) Interferon-alfa polypeptider og konjugater
EP1576117A3 (de) Verfahren zur gewinnung lebensfähiger menschlicher leberzellen, einschliesslich hepatischer stamm-/vorläuferzellen
WO2002018572A3 (en) Membrane penetrating peptides and uses thereof
TW200505501A (en) CCI-779 lyophilized formulations
EA200701293A1 (ru) Терапевтическая композиция фактора роста кератиноцитов
WO2005084223A3 (en) Methods and compositions for hair growth
WO2007003324A3 (de) Zellfreies transplantat aus matrix und serum
WO2004031343A3 (en) Human foreskin cells suitable for culturing stem cells
WO2001034824A3 (en) Methods and compositions comprising renilla gfp
NO20031251D0 (no) Fremgangsmåte for fremstilling av små soyabaserte måltid med lavt fett- oghöyt proteininnhold
WO2004081033A3 (en) Synthesis of proteins by cell-free protein expression
WO2005105899A3 (fr) Gel concentre en polyester sulfonique ramifie et procede de preparation de ce gel
WO2005019471A3 (en) Periostin-like factor: compositions and methods for making and using the same
TW200420530A (en) Novel method for preparing methyl 2-diphenylmethylsulfinylacetate
AU2002221814A1 (en) Cell constructs which can be obtained from mesenschymal stem cells and cells derivable therefrom and the use thereof
BRPI0403200A (pt) Método para recrescimento de células embriogênicas crioconservadas de conìferas

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee