DE60313894D1 - Verfahren zum nachweis eines analyten in einer probe - Google Patents
Verfahren zum nachweis eines analyten in einer probeInfo
- Publication number
- DE60313894D1 DE60313894D1 DE60313894T DE60313894T DE60313894D1 DE 60313894 D1 DE60313894 D1 DE 60313894D1 DE 60313894 T DE60313894 T DE 60313894T DE 60313894 T DE60313894 T DE 60313894T DE 60313894 D1 DE60313894 D1 DE 60313894D1
- Authority
- DE
- Germany
- Prior art keywords
- analyte
- solid support
- oligo nucleotides
- probe
- analytes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012491 analyte Substances 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 4
- 239000000523 sample Substances 0.000 abstract 8
- 108091034117 Oligonucleotide Proteins 0.000 abstract 7
- 239000007787 solid Substances 0.000 abstract 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 6
- 238000001514 detection method Methods 0.000 abstract 3
- 238000003556 assay Methods 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02028582 | 2002-12-20 | ||
| EP02028582A EP1431398A1 (de) | 2002-12-20 | 2002-12-20 | Verfahren zum Nachweis die Menge eines Analyten in einer Mischung |
| US43943903P | 2003-01-13 | 2003-01-13 | |
| US439439P | 2003-01-13 | ||
| PCT/EP2003/014661 WO2004057023A1 (en) | 2002-12-20 | 2003-12-19 | A method for detecting an analyte in a sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60313894D1 true DE60313894D1 (de) | 2007-06-28 |
| DE60313894T2 DE60313894T2 (de) | 2008-01-17 |
Family
ID=32338069
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60313894T Expired - Lifetime DE60313894T2 (de) | 2002-12-20 | 2003-12-19 | Verfahren zum nachweis eines analyten in einer probe |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20060188880A1 (de) |
| EP (2) | EP1431398A1 (de) |
| AT (1) | ATE362549T1 (de) |
| AU (1) | AU2003290106A1 (de) |
| DE (1) | DE60313894T2 (de) |
| WO (1) | WO2004057023A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7223544B2 (en) | 2003-06-27 | 2007-05-29 | Cornell Research Foundation, Inc. | Nucleic acid-engineered materials |
| US20050255044A1 (en) * | 2004-05-14 | 2005-11-17 | Lomnes Stephen J | Contrast agent for combined modality imaging and methods and systems thereof |
| ATE529533T1 (de) * | 2004-06-11 | 2011-11-15 | Evotec Ag | Verfahren zum nachweis von analyten in einer probe |
| US7465561B2 (en) * | 2005-06-30 | 2008-12-16 | Roche Molecular Systems, Inc. | Probes and methods for hepatitis C virus typing using single probe analysis |
| US8486621B2 (en) | 2005-08-11 | 2013-07-16 | Cornell Research Foundation, Inc. | Nucleic acid-based matrixes |
| DE102006009516B4 (de) * | 2006-02-28 | 2007-12-06 | Analyticon Biotechnologies Ag | Testvorrichtung, Herstellungsverfahren dafür und Testverfahren |
| DE102008062372B3 (de) | 2008-12-17 | 2010-06-17 | Medizinische Hochschule Hannover | Nachweiskonjugat und Verfahren zur Analyse |
| WO2011090445A1 (en) * | 2010-01-22 | 2011-07-28 | Huseyin Avni Oktem | Method for detection of non-labeled pcr products on sandwich hybridization based array platforms |
| US10190986B2 (en) * | 2011-06-06 | 2019-01-29 | Abbott Laboratories | Spatially resolved ligand-receptor binding assays |
| US20150045249A1 (en) * | 2012-02-27 | 2015-02-12 | Toray Industries, Inc. | Nucleic acid detection method |
| WO2014083474A1 (en) * | 2012-11-30 | 2014-06-05 | Koninklijke Philips N.V. | Capture particle for selectively binding a target molecule |
| WO2014113502A1 (en) * | 2013-01-15 | 2014-07-24 | Quanterix Corporation | Detection of dna or rna using single molecule arrays and other techniques |
| US20190376963A1 (en) * | 2018-05-04 | 2019-12-12 | Abbott Laboratories | Sequential sampling method for improving immunoassay sensitivity and kinetics of small volume samples |
| CN111394432B (zh) * | 2020-03-27 | 2023-03-24 | 深圳闪量科技有限公司 | 基于通用探针芯片的多重定量pcr检测系统 |
| CA3186950A1 (en) * | 2020-07-23 | 2022-01-27 | Scott Benson | Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same |
| EP4341671A1 (de) * | 2021-05-19 | 2024-03-27 | Leica Microsystems CMS GmbH | Verfahren zur analyse einer biologischen probe oder einer chemischen verbindung oder eines chemischen elements |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990001564A1 (en) * | 1988-08-09 | 1990-02-22 | Microprobe Corporation | Methods for multiple target analyses through nucleic acid hybridization |
| US5784162A (en) * | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| US6861264B2 (en) * | 1992-01-27 | 2005-03-01 | Cis Bio International | Method of measuring the luminescence emitted in a luminescent assay |
| AU697841B2 (en) | 1993-11-12 | 1998-10-15 | Phri Properties, Inc. | Hybridization probes for nucleic acid detection, universal stems, methods and kits |
| US5750356A (en) * | 1996-05-31 | 1998-05-12 | Anergen, Inc. | Method for monitoring T cell reactivity |
| US6361944B1 (en) * | 1996-07-29 | 2002-03-26 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| US6544730B1 (en) * | 1997-10-27 | 2003-04-08 | Prescott Deininger | High density polymorphic genetic locus |
| US6251615B1 (en) * | 1998-02-20 | 2001-06-26 | Cell Analytics, Inc. | Cell analysis methods |
| US6309833B1 (en) * | 1999-04-12 | 2001-10-30 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures |
| IL150113A0 (en) * | 1999-12-15 | 2002-12-01 | Peptor Ltd | Fragments and antagonists of heat shock protein 60 |
| US20030082571A1 (en) * | 2001-04-10 | 2003-05-01 | Kachab Edward Hanna | Linear nucleic acid and sequence therefor |
| US7172804B2 (en) * | 2001-07-17 | 2007-02-06 | Northwestern University | Film-immobilized capture particles |
| US7221785B2 (en) * | 2002-05-21 | 2007-05-22 | Agilent Technologies, Inc. | Method and system for measuring a molecular array background signal from a continuous background region of specified size |
| US7202036B2 (en) * | 2003-06-12 | 2007-04-10 | Los Alamos National Security, Llc | Quenching methods for background reduction in luminescence-based probe-target binding assays |
| EP1829964A4 (de) * | 2004-12-08 | 2009-03-04 | Takeshi Yamamoto | Verfahren zur untersuchung einer gensequenz |
-
2002
- 2002-12-20 EP EP02028582A patent/EP1431398A1/de not_active Withdrawn
-
2003
- 2003-12-19 WO PCT/EP2003/014661 patent/WO2004057023A1/en active IP Right Grant
- 2003-12-19 EP EP03782468A patent/EP1585829B1/de not_active Expired - Lifetime
- 2003-12-19 AU AU2003290106A patent/AU2003290106A1/en not_active Abandoned
- 2003-12-19 US US10/539,628 patent/US20060188880A1/en not_active Abandoned
- 2003-12-19 DE DE60313894T patent/DE60313894T2/de not_active Expired - Lifetime
- 2003-12-19 AT AT03782468T patent/ATE362549T1/de not_active IP Right Cessation
-
2010
- 2010-05-10 US US12/662,896 patent/US8580577B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| DE60313894T2 (de) | 2008-01-17 |
| EP1431398A1 (de) | 2004-06-23 |
| AU2003290106A1 (en) | 2004-07-14 |
| WO2004057023A1 (en) | 2004-07-08 |
| EP1585829A1 (de) | 2005-10-19 |
| US20060188880A1 (en) | 2006-08-24 |
| ATE362549T1 (de) | 2007-06-15 |
| US20110105348A1 (en) | 2011-05-05 |
| EP1585829B1 (de) | 2007-05-16 |
| US8580577B2 (en) | 2013-11-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |