ATE168724T1 - Nachweis eines polynukleotids durch ersätzung einer kette an einer fangsonde - Google Patents

Nachweis eines polynukleotids durch ersätzung einer kette an einer fangsonde

Info

Publication number
ATE168724T1
ATE168724T1 AT95108635T AT95108635T ATE168724T1 AT E168724 T1 ATE168724 T1 AT E168724T1 AT 95108635 T AT95108635 T AT 95108635T AT 95108635 T AT95108635 T AT 95108635T AT E168724 T1 ATE168724 T1 AT E168724T1
Authority
AT
Austria
Prior art keywords
polynucleotide
capture probe
support
nucleic acid
label
Prior art date
Application number
AT95108635T
Other languages
English (en)
Inventor
Michael S Urdea
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/251,152 external-priority patent/US5118605A/en
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of ATE168724T1 publication Critical patent/ATE168724T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95108635T 1988-09-29 1988-10-03 Nachweis eines polynukleotids durch ersätzung einer kette an einer fangsonde ATE168724T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/251,152 US5118605A (en) 1984-10-16 1988-09-29 Polynucleotide determination with selectable cleavage sites

Publications (1)

Publication Number Publication Date
ATE168724T1 true ATE168724T1 (de) 1998-08-15

Family

ID=22950701

Family Applications (2)

Application Number Title Priority Date Filing Date
AT88309203T ATE133714T1 (de) 1988-09-29 1988-10-03 Polynukleotid-bestimmung mit selektierbaren spaltstellen
AT95108635T ATE168724T1 (de) 1988-09-29 1988-10-03 Nachweis eines polynukleotids durch ersätzung einer kette an einer fangsonde

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT88309203T ATE133714T1 (de) 1988-09-29 1988-10-03 Polynukleotid-bestimmung mit selektierbaren spaltstellen

Country Status (3)

Country Link
EP (1) EP0703296B1 (de)
AT (2) ATE133714T1 (de)
DE (1) DE3856224T2 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
CA2289925A1 (en) 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
WO1999024612A2 (en) 1997-11-06 1999-05-20 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
US6287772B1 (en) 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
AU760236B2 (en) 1998-06-18 2003-05-08 Exact Sciences Corporation Denaturing gradient affinity electrophoresis and methods of use thereof
US6238927B1 (en) 1998-10-05 2001-05-29 Mosaic Technologies, Incorporated Reverse displacement assay for detection of nucleic acid sequences
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
EP1520045A1 (de) * 2002-06-03 2005-04-06 PamGene B.V. Neue biochips mit höher dichte und verfahren zur analyse von analyten
US7340957B2 (en) 2004-07-29 2008-03-11 Los Alamos National Security, Llc Ultrasonic analyte concentration and application in flow cytometry
ATE538377T1 (de) 2007-04-02 2012-01-15 Acoustic Cytometry Systems Inc Verfahren zur verbesserten analyse von in einem akustischen feld fokussierten zellen und partikeln
US8266950B2 (en) 2007-12-19 2012-09-18 Los Alamos National Security, LLP Particle analysis in an acoustic cytometer
JP2012506705A (ja) 2008-10-27 2012-03-22 キアジェン ゲイサーズバーグ インコーポレイテッド 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム
CN102414327B (zh) 2009-05-01 2015-04-08 奇亚根盖瑟斯堡股份有限公司 用于在样品中检测rna剪接形式的非靶向扩增方法
WO2011032124A1 (en) 2009-09-14 2011-03-17 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
JP6103941B2 (ja) 2010-01-29 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド 核酸の配列特異的精製および多重分析のための方法および組成物
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
KR102929834B1 (ko) 2018-04-03 2026-02-23 더 로얄 인스티튜션 포 디 어드밴스먼트 오브 러닝/맥길 유니버시티 결합에 의한 공국재화 샌드위치 어세이
WO2021064460A1 (en) 2019-10-02 2021-04-08 The Royal Institution For The Advancement Of Learning/Mcgill University Colocalization-by-linkage sandwich assays for multiplexing

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3853914A (en) 1971-01-11 1974-12-10 Syva Co Ligand determination of spin labeled compounds by receptor displacement
US4174384A (en) 1975-06-30 1979-11-13 Syva Company Fluorescence quenching with immunological pairs in immunoassays
IT1079045B (it) 1977-05-16 1985-05-08 Syva Co Analisi imuunologica fluorescente a doppio ricettore
US4160645A (en) 1977-07-14 1979-07-10 Syva Company Catalyst mediated competitive protein binding assay
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4318890A (en) 1978-10-02 1982-03-09 Monahan Daniel E Reactor for generating granular metal hydride
US4318707A (en) 1978-11-24 1982-03-09 Syva Company Macromolecular fluorescent quencher particle in specific receptor assays
US4358535A (en) 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
FR2519005B1 (fr) 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
US4766064A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
AU6834187A (en) * 1985-12-17 1987-07-15 Genetics Institute Inc. Displacement polynucleotide method and reagent complex
DE3785658T2 (de) * 1986-08-11 1993-08-12 Siska Diagnostics Inc Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
DE3681176D1 (de) * 1986-12-01 1991-10-02 Molecular Biosystems Inc Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.

Also Published As

Publication number Publication date
DE3856224D1 (de) 1998-08-27
EP0703296B1 (de) 1998-07-22
EP0703296A1 (de) 1996-03-27
DE3856224T2 (de) 1998-12-03
ATE133714T1 (de) 1996-02-15

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