DE60237194D1 - Plattform für protein-profiling - Google Patents

Plattform für protein-profiling

Info

Publication number
DE60237194D1
DE60237194D1 DE60237194T DE60237194T DE60237194D1 DE 60237194 D1 DE60237194 D1 DE 60237194D1 DE 60237194 T DE60237194 T DE 60237194T DE 60237194 T DE60237194 T DE 60237194T DE 60237194 D1 DE60237194 D1 DE 60237194D1
Authority
DE
Germany
Prior art keywords
active
activity
methods
proteins
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60237194T
Other languages
English (en)
Inventor
Matthew Patricelli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Activx Biosciences Inc
Original Assignee
Activx Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Activx Biosciences Inc filed Critical Activx Biosciences Inc
Application granted granted Critical
Publication of DE60237194D1 publication Critical patent/DE60237194D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors
    • G01N30/82—Automatic means therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60237194T 2001-03-02 2002-03-01 Plattform für protein-profiling Expired - Lifetime DE60237194D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27300701P 2001-03-02 2001-03-02
PCT/US2002/006234 WO2002071066A1 (en) 2001-03-02 2002-03-01 Protein profiling platform

Publications (1)

Publication Number Publication Date
DE60237194D1 true DE60237194D1 (de) 2010-09-16

Family

ID=23042159

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60237194T Expired - Lifetime DE60237194D1 (de) 2001-03-02 2002-03-01 Plattform für protein-profiling

Country Status (5)

Country Link
US (1) US7179655B2 (de)
EP (1) EP1370867B1 (de)
AT (1) ATE476654T1 (de)
DE (1) DE60237194D1 (de)
WO (1) WO2002071066A1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2053406A3 (de) * 2001-07-16 2009-06-24 caprotec bioanalytics GmbH Capture Compounds, Sammlung derselben und Verfahren zur Proteomanalyse und Komplexzusammensetzung
AU2003225799A1 (en) * 2002-03-03 2003-09-29 Activx Biosciences, Inc. Tethered activity-based probes and uses thereof
AU2003286553A1 (en) * 2002-10-18 2004-05-04 Promega Corporation Methods for separating molecules
CA2658334C (en) * 2003-01-16 2012-07-10 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
GB0317123D0 (en) * 2003-07-22 2003-08-27 Xzillion Gmbh & Co Kg Characterising polypeptides
US8796037B2 (en) * 2003-12-11 2014-08-05 Kazuhiro Imai Method of detection, separation and identification for expressed trace protein/peptide
US20080305958A1 (en) * 2004-04-30 2008-12-11 Mark Hayward Process for Liquid or gas Chromatography/Mass Spectrometry Based Biomolecular Screening for Drug Discovery
WO2006096704A2 (en) 2005-03-07 2006-09-14 Invitrogen Corporation Isotopically-labeled proteome standards
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
US8673267B2 (en) 2009-03-02 2014-03-18 Massachusetts Institute Of Technology Methods and products for in vivo enzyme profiling
NZ621655A (en) 2009-10-26 2015-08-28 Nestec Sa Assays for the detection of anti-tnf drugs and autoantibodies
GB2495036B (en) 2010-07-07 2019-03-27 Thermo Fisher Scient Gmbh Kits for analyte mass spectrometry quantitation using a universal reporter
CA3214092A1 (en) 2011-03-15 2012-09-20 Massachusetts Institute Of Technology Multiplexed detection with isotope-coded reporters
CN103782172A (zh) 2011-07-06 2014-05-07 雀巢产品技术援助有限公司 检测针对使用TNFα的生物疗法的中和性自体抗体的测定法
CA2841752A1 (en) 2011-07-14 2013-06-13 The George Washington University Plume collimation for laser ablation electrospray ionization mass spectrometry
CN105452481A (zh) 2013-06-07 2016-03-30 麻省理工学院 基于亲和力检测配体编码的合成性生物标记物
WO2014197754A1 (en) 2013-06-07 2014-12-11 Pierce Biotechnology, Inc. Absolute quantitation of proteins and protein modifications by mass spectrometry with multiplexed internal standards
GB201404756D0 (en) * 2014-03-17 2014-04-30 Analytica Llc Q Profiling apparatus and method
MX2017007232A (es) 2014-12-05 2017-10-16 Nestec Sa Ensayos de desplazamiento por movilidad homogeneos indirectos para la deteccion de productos biologicos en muestras de pacientes.
WO2017031378A1 (en) * 2015-08-18 2017-02-23 Krusemark Casey J Systems and methods for proteomic activity analysis using dna-encoded probes
US11448643B2 (en) 2016-04-08 2022-09-20 Massachusetts Institute Of Technology Methods to specifically profile protease activity at lymph nodes
CA3022928A1 (en) 2016-05-05 2017-11-09 Massachusetts Institute Of Technology Methods and uses for remotely triggered protease activity measurements
US20180128832A1 (en) * 2016-11-09 2018-05-10 Richard-Allan Scientific Company Proteogenomic analysis system and methods
AU2018248327A1 (en) 2017-04-07 2019-10-17 Massachusetts Institute Of Technology Methods to spatially profile protease activity in tissue and sections
WO2019173332A1 (en) 2018-03-05 2019-09-12 Massachusetts Institute Of Technology Inhalable nanosensors with volatile reporters and uses thereof
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
US11732009B2 (en) 2018-06-08 2023-08-22 Glympse Bio, Inc. Activity sensor with tunable analyte
US11028425B2 (en) 2018-06-08 2021-06-08 Glympse Bio, Inc. Diagnosis and monitoring of liver disease
EP3911753A1 (de) 2019-01-17 2021-11-24 Massachusetts Institute of Technology Sensoren zur erfassung und abbildung von krebsmetastasen

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4433051A (en) 1981-10-14 1984-02-21 Yeda Research And Development Co., Ltd. Derivatives of α-difluoromethylornithine useful in analysis
US4415655A (en) 1982-05-17 1983-11-15 Techamerica Group, Inc. Electrophoretic separation of isoenzymes utilizing a stable polyacrylamide system
US4481094A (en) 1982-05-17 1984-11-06 Techamerica Group, Inc. Stabilized polyacrylamide gels and system for SDS electrophoresis
US4865707A (en) 1986-10-21 1989-09-12 Northeastern University Capillary gel electrophoresis columns
US4946794A (en) 1986-12-18 1990-08-07 Protein Databases, Inc. Visualization of proteins on electrophoresis gels using planar dyes
US5041538A (en) 1987-08-28 1991-08-20 The Salk Institute For Biological Studies Mammalian follistatin
PT97321A (pt) 1990-04-11 1992-01-31 Indu Parikh Processo de purificacao de factor de crescimento epidermico
US5290920A (en) 1992-04-16 1994-03-01 Allelix Biopharmaceuticals Inc. Method of purifying human epidermal growth factor
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
ATE242485T1 (de) 1993-05-28 2003-06-15 Baylor College Medicine Verfahren und massenspektrometer zur desorption und ionisierung von analyten
US5856082A (en) * 1994-08-31 1999-01-05 University Of British Columbia Devices and methods for characterizing proteins and peptides
US6127134A (en) 1995-04-20 2000-10-03 Carnegie Mellon University Difference gel electrophoresis using matched multiple dyes
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
GB9717926D0 (en) 1997-08-22 1997-10-29 Micromass Ltd Methods and apparatus for tandem mass spectrometry
US6207370B1 (en) * 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
EP1050065A4 (de) 1998-01-23 2004-03-31 Analytica Of Branford Inc Oberflächen-massenspektrometrie
ATE253126T1 (de) * 1998-08-25 2003-11-15 Univ Washington Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
US6379970B1 (en) * 1999-04-30 2002-04-30 The Arizona Board Of Regents On Behalf Of The University Of Arizona Analysis of differential protein expression
CA2403296A1 (en) 2000-04-10 2001-10-18 Benjamin F. Cravatt Proteomic analysis using activity-based probe libraries
AU2002232681A1 (en) * 2000-11-10 2002-05-21 The Regents Of The Univeristy Of California Activity-dependent cysteine protease profiling reagent

Also Published As

Publication number Publication date
EP1370867A1 (de) 2003-12-17
ATE476654T1 (de) 2010-08-15
US7179655B2 (en) 2007-02-20
WO2002071066A1 (en) 2002-09-12
EP1370867B1 (de) 2010-08-04
US20020182651A1 (en) 2002-12-05
EP1370867A4 (de) 2005-01-26

Similar Documents

Publication Publication Date Title
DE60237194D1 (de) Plattform für protein-profiling
Ploug et al. Peptide-derived antagonists of the urokinase receptor. affinity maturation by combinatorial chemistry, identification of functional epitopes, and inhibitory effect on cancer cell intravasation
Angel et al. Mapping extracellular matrix proteins in formalin-fixed, paraffin-embedded tissues by MALDI imaging mass spectrometry
Sauls et al. Modification of fibrinogen by homocysteine thiolactone increases resistance to fibrinolysis: a potential mechanism of the thrombotic tendency in hyperhomocysteinemia
Thomadaki et al. Whole-saliva proteolysis and its impact on salivary diagnostics
O'Leary et al. MYBPC3 truncation mutations enhance actomyosin contractile mechanics in human hypertrophic cardiomyopathy
WO2002063271A3 (en) Activity based probe analysis
Camporeale et al. K8 and K12 are biotinylated in human histone H4
Kaneko et al. Comparison of matrix proteins in different types of urinary stone by proteomic analysis using liquid chromatography–tandem mass spectrometry
ATE463512T1 (de) Identifizierung und charakterisierung von funktionsblockierenden anti-ed-b-fibronektin antikörpern
WO2005010215A3 (en) Methods for identifying tolerance modulatory compounds and uses therefor
FR2827866B1 (fr) Peptides synthetiques ou naturels liant la proteine phosphatase 2a, methode d'identification et utilisations
Rengasamy et al. Bioactive peptides and proteins as alternative antiplatelet drugs
Sabo et al. Conformational Analysis of γ ‘Peptide (410− 427) Interactions with Thrombin Anion Binding Exosite II
WO2003028543A3 (en) Nucleic acid molecules, polypeptides and uses therefor, including diagnosis and treatment of alzheimer's disease
Steen et al. Analysis of bromotryptophan and hydroxyproline modifications by high-resolution, high-accuracy precursor ion scanning utilizing fragment ions with mass-deficient mass tags
DE60034966D1 (de) Auswertung von biologischen mitteln in lebenden zielzellen
DE69811348D1 (de) Verfahren zur modulierung des wachstums kollateraler arterien und / oder anderer arterien aus bestehenden arteriolen verbindungen
DE69931482D1 (de) Isolierte peptide die aminosäuresequenzen von ny-eso-1 entsprechen und an mhc-klasse i und mhc klasse ii moleküle binden, und deren verwendungen
Farrugia et al. Analysis of a soluble (UreD: UreF: UreG) 2 accessory protein complex and its interactions with Klebsiella aerogenes urease by mass spectrometry
MXPA05011085A (es) Composiciones y metodos relacionados a stop-1.
DE69841322D1 (de) Hepatitis c rezeptorprotein cd81
BRPI0509857A (pt) agrecanase e proteìna isolada ou recombinante, polinucleotìdeo, kit ou sistema de ensaio, método para identificar um composto capaz de modular a atividade de uma agrecanase, anticorpo, composição, célula hospedeira, método para produzir agrecanase truncada purificada, e, método para o tratamento de uma condição inflamatória em um paciente
ATE384263T1 (de) Verfahren zur bestimmung von biologischen agenzien in lebenden zielzellen
DE50212280D1 (de) Verwendung von hmgb-proteinen und dafür codierenden nukleinsäuren

Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: MARKS & CLERK (LUXEMBOURG) LLP, LUXEMBOURG, LU