DE60237194D1 - Plattform für protein-profiling - Google Patents

Plattform für protein-profiling

Info

Publication number
DE60237194D1
DE60237194D1 DE60237194T DE60237194T DE60237194D1 DE 60237194 D1 DE60237194 D1 DE 60237194D1 DE 60237194 T DE60237194 T DE 60237194T DE 60237194 T DE60237194 T DE 60237194T DE 60237194 D1 DE60237194 D1 DE 60237194D1
Authority
DE
Germany
Prior art keywords
active
activity
methods
proteins
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60237194T
Other languages
English (en)
Inventor
Matthew Patricelli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Activx Biosciences Inc
Original Assignee
Activx Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Activx Biosciences Inc filed Critical Activx Biosciences Inc
Application granted granted Critical
Publication of DE60237194D1 publication Critical patent/DE60237194D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors
    • G01N30/82—Automatic means therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60237194T 2001-03-02 2002-03-01 Plattform für protein-profiling Expired - Lifetime DE60237194D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27300701P 2001-03-02 2001-03-02
PCT/US2002/006234 WO2002071066A1 (en) 2001-03-02 2002-03-01 Protein profiling platform

Publications (1)

Publication Number Publication Date
DE60237194D1 true DE60237194D1 (de) 2010-09-16

Family

ID=23042159

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60237194T Expired - Lifetime DE60237194D1 (de) 2001-03-02 2002-03-01 Plattform für protein-profiling

Country Status (5)

Country Link
US (1) US7179655B2 (de)
EP (1) EP1370867B1 (de)
AT (1) ATE476654T1 (de)
DE (1) DE60237194D1 (de)
WO (1) WO2002071066A1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2652991A1 (en) * 2001-07-16 2003-11-13 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
WO2003079014A1 (en) * 2002-03-03 2003-09-25 Activx Biosciences, Inc. Tethered activity-based probes and uses thereof
AU2003286553A1 (en) * 2002-10-18 2004-05-04 Promega Corporation Methods for separating molecules
DK2259068T3 (en) * 2003-01-16 2013-11-11 Caprotec Bioanalytics Gmbh Capture compounds and methods for analyzing the proteome
GB0317123D0 (en) * 2003-07-22 2003-08-27 Xzillion Gmbh & Co Kg Characterising polypeptides
JP4679368B2 (ja) * 2003-12-11 2011-04-27 一洋 今井 発現微量タンパク質／ペプチドの検出・分離・同定法
EP1747455A2 (de) * 2004-04-30 2007-01-31 Mark Hayward Verfahren zum auf flüssigkeits- oder gaschromatographie/massenspektrometrie beruhenden biomolekularen screening mit hohem durchsatz für die arzneistoffindung
US7939331B2 (en) 2005-03-07 2011-05-10 Life Technologies Corporation Isotopically-labeled proteome standards
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
EP2403964B1 (de) 2009-03-02 2021-09-08 Massachusetts Institute of Technology Verfahren und produkte zur in-vivo-profilerstellung von enzymen
CN104634955B (zh) 2009-10-26 2016-09-14 雀巢产品技术援助有限公司 检测抗-tnf药物和自身抗体的试验
US9297808B2 (en) 2010-07-07 2016-03-29 Thermo Fisher Scientific Gmbh Analyte mass spectrometry quantitation using a universal reporter
EP3950704A1 (de) 2011-03-15 2022-02-09 Massachusetts Institute Of Technology Multiplexierte erkennung mit isotopencodierten reportern
AU2012278802B2 (en) 2011-07-06 2015-11-26 Société des Produits Nestlé S.A. Assays for detecting neutralizing autoantibodies to biologic therapy with TNF alpha
US8829426B2 (en) 2011-07-14 2014-09-09 The George Washington University Plume collimation for laser ablation electrospray ionization mass spectrometry
CN109813914A (zh) 2013-06-07 2019-05-28 皮尔斯生物科技有限公司 通过质谱分析用复用内标物对蛋白质和蛋白质修饰的绝对定量
CA2914754A1 (en) 2013-06-07 2014-12-11 Massachusetts Institute Of Technology Affinity-based detection of ligand-encoded synthetic biomarkers
GB201404756D0 (en) * 2014-03-17 2014-04-30 Analytica Llc Q Profiling apparatus and method
SG11201704200SA (en) 2014-12-05 2017-06-29 Nestec Sa Indirect homogeneous mobility shift assays for the detection of biologics in patient samples
WO2017031378A1 (en) * 2015-08-18 2017-02-23 Krusemark Casey J Systems and methods for proteomic activity analysis using dna-encoded probes
WO2017177115A1 (en) 2016-04-08 2017-10-12 Massachusetts Institute Of Technology Methods to specifically profile protease activity at lymph nodes
CA3022928A1 (en) 2016-05-05 2017-11-09 Massachusetts Institute Of Technology Methods and uses for remotely triggered protease activity measurements
US20180128832A1 (en) * 2016-11-09 2018-05-10 Richard-Allan Scientific Company Proteogenomic analysis system and methods
CA3059358A1 (en) 2017-04-07 2018-10-11 Massachusetts Institute Of Technology Methods to spatially profile protease activity in tissue and sections
US11054428B2 (en) 2018-03-05 2021-07-06 Massachusetts Institute Of Technology Inhalable nanosensors with volatile reporters and uses thereof
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
US11028425B2 (en) 2018-06-08 2021-06-08 Glympse Bio, Inc. Diagnosis and monitoring of liver disease
US11732009B2 (en) 2018-06-08 2023-08-22 Glympse Bio, Inc. Activity sensor with tunable analyte
EP3911753A1 (de) 2019-01-17 2021-11-24 Massachusetts Institute of Technology Sensoren zur erfassung und abbildung von krebsmetastasen

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4433051A (en) * 1981-10-14 1984-02-21 Yeda Research And Development Co., Ltd. Derivatives of α-difluoromethylornithine useful in analysis
US4415655A (en) * 1982-05-17 1983-11-15 Techamerica Group, Inc. Electrophoretic separation of isoenzymes utilizing a stable polyacrylamide system
US4481094A (en) * 1982-05-17 1984-11-06 Techamerica Group, Inc. Stabilized polyacrylamide gels and system for SDS electrophoresis
US4865707A (en) * 1986-10-21 1989-09-12 Northeastern University Capillary gel electrophoresis columns
US4946794A (en) * 1986-12-18 1990-08-07 Protein Databases, Inc. Visualization of proteins on electrophoresis gels using planar dyes
US5041538A (en) * 1987-08-28 1991-08-20 The Salk Institute For Biological Studies Mammalian follistatin
AU7779191A (en) 1990-04-11 1991-10-30 Indu Parikh Method for the purification of epidermal growth factor
US5290920A (en) * 1992-04-16 1994-03-01 Allelix Biopharmaceuticals Inc. Method of purifying human epidermal growth factor
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
AU676582B2 (en) * 1993-05-28 1997-03-13 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US5856082A (en) 1994-08-31 1999-01-05 University Of British Columbia Devices and methods for characterizing proteins and peptides
US6127134A (en) * 1995-04-20 2000-10-03 Carnegie Mellon University Difference gel electrophoresis using matched multiple dyes
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
GB9717926D0 (en) * 1997-08-22 1997-10-29 Micromass Ltd Methods and apparatus for tandem mass spectrometry
US6207370B1 (en) * 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
EP1050065A4 (de) * 1998-01-23 2004-03-31 Analytica Of Branford Inc Oberflächen-massenspektrometrie
EP1105517B2 (de) * 1998-08-25 2009-09-30 University Of Washington Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
US6379970B1 (en) 1999-04-30 2002-04-30 The Arizona Board Of Regents On Behalf Of The University Of Arizona Analysis of differential protein expression
US20020045194A1 (en) * 2000-04-10 2002-04-18 Cravatt Benjamin F. Proteomic analysis
WO2002038540A2 (en) * 2000-11-10 2002-05-16 The Regents Of The Univeristy Of California Activity-dependent cysteine protease profiling reagent

Also Published As

Publication number Publication date
EP1370867A4 (de) 2005-01-26
EP1370867A1 (de) 2003-12-17
ATE476654T1 (de) 2010-08-15
EP1370867B1 (de) 2010-08-04
US20020182651A1 (en) 2002-12-05
US7179655B2 (en) 2007-02-20
WO2002071066A1 (en) 2002-09-12

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Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: MARKS & CLERK (LUXEMBOURG) LLP, LUXEMBOURG, LU