DE60223276D1 - Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen - Google Patents
Verfahren zur Blockierung von unspezifischen Hybridisierungen von NukleinsäuresequenzenInfo
- Publication number
- DE60223276D1 DE60223276D1 DE60223276T DE60223276T DE60223276D1 DE 60223276 D1 DE60223276 D1 DE 60223276D1 DE 60223276 T DE60223276 T DE 60223276T DE 60223276 T DE60223276 T DE 60223276T DE 60223276 D1 DE60223276 D1 DE 60223276D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- acid sequences
- blocking nonspecific
- nonspecific hybridizations
- hybridizations
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US31611601P | 2001-08-31 | 2001-08-31 | |
US316116P | 2001-08-31 | ||
PCT/US2002/027799 WO2003020902A2 (en) | 2001-08-31 | 2002-09-03 | Methods for blocking nonspecific hybridizations of nucleic acid sequences |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60223276D1 true DE60223276D1 (de) | 2007-12-13 |
DE60223276T2 DE60223276T2 (de) | 2008-08-14 |
Family
ID=23227533
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60223276T Expired - Lifetime DE60223276T2 (de) | 2001-08-31 | 2002-09-03 | Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen |
Country Status (6)
Country | Link |
---|---|
US (1) | US20040009487A1 (de) |
EP (1) | EP1438435B1 (de) |
JP (1) | JP2005502346A (de) |
AU (1) | AU2002332778A1 (de) |
DE (1) | DE60223276T2 (de) |
WO (1) | WO2003020902A2 (de) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6780591B2 (en) * | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US7501245B2 (en) * | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US20020072060A1 (en) * | 2000-07-19 | 2002-06-13 | Getts Robert C. | Methods for detecting and assaying nucleic acid sequences |
WO2001066555A1 (en) * | 2000-03-08 | 2001-09-13 | Datascope Investment Corp. | Methods for assay and detection on a microarray |
US20040115692A1 (en) * | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
JP2004523243A (ja) * | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
US7094537B2 (en) * | 2002-04-30 | 2006-08-22 | Agilent Technologies, Inc. | Micro arrays with structured and unstructured probes |
US20040185451A1 (en) * | 2003-03-21 | 2004-09-23 | Leproust Eric M. | Methods for detecting the presence of a nucleic acid analyte in a sample |
US20050170367A1 (en) * | 2003-06-10 | 2005-08-04 | Quake Stephen R. | Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids |
ATE373729T1 (de) * | 2003-07-03 | 2007-10-15 | Danmarks Og Groenlands Geol Un | Verfahren zur selektiven detektion einer zielnukleinsäure |
US7169560B2 (en) * | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US20060172408A1 (en) * | 2003-12-01 | 2006-08-03 | Quake Steven R | Device for immobilizing chemical and biochemical species and methods of using same |
WO2005080605A2 (en) | 2004-02-19 | 2005-09-01 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
US20050239085A1 (en) * | 2004-04-23 | 2005-10-27 | Buzby Philip R | Methods for nucleic acid sequence determination |
US20050260609A1 (en) * | 2004-05-24 | 2005-11-24 | Lapidus Stanley N | Methods and devices for sequencing nucleic acids |
US7476734B2 (en) * | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
US20070117103A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
US20070117104A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
DE602005027700D1 (de) * | 2004-05-25 | 2011-06-09 | Helicos Biosciences Corp | Verfahren zur nukleinsäureimmobilisierung |
US20060024678A1 (en) * | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
US20060118754A1 (en) * | 2004-12-08 | 2006-06-08 | Lapen Daniel C | Stabilizing a polyelectrolyte multilayer |
US7220549B2 (en) * | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
US20060172328A1 (en) * | 2005-01-05 | 2006-08-03 | Buzby Philip R | Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction |
US7482120B2 (en) * | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
US20060263790A1 (en) * | 2005-05-20 | 2006-11-23 | Timothy Harris | Methods for improving fidelity in a nucleic acid synthesis reaction |
US7550264B2 (en) * | 2005-06-10 | 2009-06-23 | Datascope Investment Corporation | Methods and kits for sense RNA synthesis |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US20100173287A1 (en) * | 2005-10-20 | 2010-07-08 | Hitachi, Ltd. | Method of analyzing nucleic acid |
CA2630416A1 (en) | 2005-11-18 | 2007-06-28 | The Children's Mercy Hospital | Mitigation of cot-1 dna distortion in nucleic acid hybridization |
US20070117102A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
US20070128610A1 (en) * | 2005-12-02 | 2007-06-07 | Buzby Philip R | Sample preparation method and apparatus for nucleic acid sequencing |
WO2008046056A1 (en) * | 2006-10-13 | 2008-04-17 | Welldoc Communications, Inc. | Reduction of nonspecific binding in nucleic acid assays and nucleic acid synthesis reactions |
US8293684B2 (en) * | 2006-11-29 | 2012-10-23 | Exiqon | Locked nucleic acid reagents for labelling nucleic acids |
JP4551917B2 (ja) * | 2007-07-18 | 2010-09-29 | 株式会社東芝 | ヒトチトクロームp450(cyp)2d6遺伝子変異の検出方法 |
JP5272230B2 (ja) * | 2008-07-30 | 2013-08-28 | 日鉄住金環境株式会社 | ユニバーサルな核酸プローブセットおよびその使用方法 |
JP5221248B2 (ja) * | 2008-08-26 | 2013-06-26 | 株式会社日立ハイテクノロジーズ | 高発現遺伝子由来のcDNAクローンの含有率を低減させたcDNAライブラリーの作製方法 |
WO2015045741A1 (ja) | 2013-09-26 | 2015-04-02 | 東洋鋼鈑株式会社 | ハイブリダイゼーション用バッファー組成物及びハイブリダイゼーション方法 |
JP6757942B2 (ja) | 2016-08-03 | 2020-09-23 | 東洋鋼鈑株式会社 | ハイブリダイゼーション用バッファー組成物及びハイブリダイゼーション方法 |
CN114264654B (zh) * | 2021-12-20 | 2023-06-02 | 中国农业科学院油料作物研究所 | 一种真菌毒素、辣椒素、苯并[α]芘混合污染物智能微阵列检测设备及方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5830645A (en) * | 1994-12-09 | 1998-11-03 | The Regents Of The University Of California | Comparative fluorescence hybridization to nucleic acid arrays |
EP0815121A4 (de) * | 1995-02-09 | 1999-09-01 | Univ Washington | Streptavidin mit veränderrer affinität |
US6072043A (en) * | 1996-06-04 | 2000-06-06 | Polyprobe, Inc. | Optimally fluorescent oligonucleotides |
US5922536A (en) * | 1996-10-18 | 1999-07-13 | Nivens; David E. | Method for nucleic acid isolation using supercritical fluids |
US6110676A (en) * | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
US6130065A (en) * | 1997-04-11 | 2000-10-10 | St. Jude Children's Research Hospital | Nitrobenzylmercaptopurineriboside (NBMPR)-insensitive, equilibrative, nucleoside transport protein, nucleic acids encoding the same and methods of use |
US6077673A (en) * | 1998-03-31 | 2000-06-20 | Clontech Laboratories, Inc. | Mouse arrays and kits comprising the same |
US6277568B1 (en) * | 1998-04-09 | 2001-08-21 | Incyte Genomics, Inc. | Nucleic acids encoding human ubiquitin-conjugating enzyme homologs |
US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
CA2368135C (en) * | 1999-03-18 | 2010-06-08 | Exiqon A/S | Xylo-lna analogues |
IL145422A0 (en) * | 1999-03-18 | 2002-06-30 | Exiqon As | One step sample preparation and detection of nucleic acids in complex in biological samples |
WO2001066555A1 (en) * | 2000-03-08 | 2001-09-13 | Datascope Investment Corp. | Methods for assay and detection on a microarray |
WO2002033125A1 (en) * | 2000-09-20 | 2002-04-25 | Datascope Investment Corp. | Nucleic acid detection by dendrimeric labeling |
US6387624B1 (en) * | 2000-04-14 | 2002-05-14 | Incyte Pharmaceuticals, Inc. | Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing |
-
2002
- 2002-09-03 US US10/234,069 patent/US20040009487A1/en not_active Abandoned
- 2002-09-03 WO PCT/US2002/027799 patent/WO2003020902A2/en active IP Right Grant
- 2002-09-03 JP JP2003525606A patent/JP2005502346A/ja active Pending
- 2002-09-03 EP EP02797818A patent/EP1438435B1/de not_active Expired - Fee Related
- 2002-09-03 DE DE60223276T patent/DE60223276T2/de not_active Expired - Lifetime
- 2002-09-03 AU AU2002332778A patent/AU2002332778A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1438435A2 (de) | 2004-07-21 |
JP2005502346A (ja) | 2005-01-27 |
EP1438435A4 (de) | 2005-11-02 |
EP1438435B1 (de) | 2007-10-31 |
WO2003020902A3 (en) | 2003-12-11 |
AU2002332778A1 (en) | 2003-03-18 |
WO2003020902A2 (en) | 2003-03-13 |
DE60223276T2 (de) | 2008-08-14 |
US20040009487A1 (en) | 2004-01-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8381 | Inventor (new situation) |
Inventor name: KADUSHIN, JAMES M., MONTVALE, NJ 07645, US Inventor name: GETTS, ROBERT C., MONTVALE, NJ 07645, US Inventor name: ZAK, ARIEH S., MONTVALE, NJ 07645, US |
|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: GENISPHERE INC., HAFTFIELD, PA., US |
|
8327 | Change in the person/name/address of the patent owner |
Owner name: GENISPHERE, LLC., HATFIELD, PA., US |