DE60223276D1 - Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen - Google Patents

Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen

Info

Publication number
DE60223276D1
DE60223276D1 DE60223276T DE60223276T DE60223276D1 DE 60223276 D1 DE60223276 D1 DE 60223276D1 DE 60223276 T DE60223276 T DE 60223276T DE 60223276 T DE60223276 T DE 60223276T DE 60223276 D1 DE60223276 D1 DE 60223276D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid sequences
blocking nonspecific
nonspecific hybridizations
hybridizations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60223276T
Other languages
English (en)
Other versions
DE60223276T2 (de
Inventor
James M Kadushin
Robert C Getts
Arieh S Zak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genisphere LLC
Original Assignee
Datascope Investment Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Datascope Investment Corp filed Critical Datascope Investment Corp
Publication of DE60223276D1 publication Critical patent/DE60223276D1/de
Application granted granted Critical
Publication of DE60223276T2 publication Critical patent/DE60223276T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60223276T 2001-08-31 2002-09-03 Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen Expired - Lifetime DE60223276T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31611601P 2001-08-31 2001-08-31
US316116P 2001-08-31
PCT/US2002/027799 WO2003020902A2 (en) 2001-08-31 2002-09-03 Methods for blocking nonspecific hybridizations of nucleic acid sequences

Publications (2)

Publication Number Publication Date
DE60223276D1 true DE60223276D1 (de) 2007-12-13
DE60223276T2 DE60223276T2 (de) 2008-08-14

Family

ID=23227533

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60223276T Expired - Lifetime DE60223276T2 (de) 2001-08-31 2002-09-03 Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen

Country Status (6)

Country Link
US (1) US20040009487A1 (de)
EP (1) EP1438435B1 (de)
JP (1) JP2005502346A (de)
AU (1) AU2002332778A1 (de)
DE (1) DE60223276T2 (de)
WO (1) WO2003020902A2 (de)

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US7875440B2 (en) * 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US20020051981A1 (en) * 2000-03-08 2002-05-02 Robert Getts Methods for assay and detection on a microarray
US20040115692A1 (en) * 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
WO2002006511A2 (en) * 2000-07-19 2002-01-24 Genisphere Inc Methods for detecting and assaying nucleic acid sequences
WO2002072892A1 (en) * 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7094537B2 (en) 2002-04-30 2006-08-22 Agilent Technologies, Inc. Micro arrays with structured and unstructured probes
US20040185451A1 (en) * 2003-03-21 2004-09-23 Leproust Eric M. Methods for detecting the presence of a nucleic acid analyte in a sample
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
WO2005003384A1 (en) * 2003-07-03 2005-01-13 Danmarks Og Grønlands Geologiske Undersøgelse Method for selective detection of a target nucleic acid
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
CA2557177A1 (en) 2004-02-19 2005-09-01 Stephen Quake Methods and kits for analyzing polynucleotide sequences
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
JP2008512084A (ja) * 2004-05-25 2008-04-24 ヘリコス バイオサイエンシーズ コーポレイション 核酸の配列決定のための方法およびデバイス
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7550264B2 (en) * 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
CN101292045A (zh) * 2005-10-20 2008-10-22 株式会社日立制作所 核酸分析方法
CA2630416A1 (en) * 2005-11-18 2007-06-28 The Children's Mercy Hospital Mitigation of cot-1 dna distortion in nucleic acid hybridization
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
US20080153094A1 (en) * 2006-10-13 2008-06-26 Minor James M Reduction of nonspecific binding in nucleic acid assays and nucleic acid synthesis reactions
US8293684B2 (en) * 2006-11-29 2012-10-23 Exiqon Locked nucleic acid reagents for labelling nucleic acids
JP4551917B2 (ja) * 2007-07-18 2010-09-29 株式会社東芝 ヒトチトクロームｐ４５０（ｃｙｐ）２ｄ６遺伝子変異の検出方法
EP2333059A4 (de) * 2008-07-30 2013-01-09 Nippon Steel Kankyo Engineering Co Ltd Universelles nukleinsäuresondenset und anwendungsvefahren dafür
JP5221248B2 (ja) * 2008-08-26 2013-06-26 株式会社日立ハイテクノロジーズ 高発現遺伝子由来のｃＤＮＡクローンの含有率を低減させたｃＤＮＡライブラリーの作製方法
US10450601B2 (en) * 2013-09-26 2019-10-22 Toyo Kohan Co., Ltd. Buffer composition for hybridization use, and hybridization method
JP6757942B2 (ja) 2016-08-03 2020-09-23 東洋鋼鈑株式会社 ハイブリダイゼーション用バッファー組成物及びハイブリダイゼーション方法
CN114264654B (zh) * 2021-12-20 2023-06-02 中国农业科学院油料作物研究所 一种真菌毒素、辣椒素、苯并[α]芘混合污染物智能微阵列检测设备及方法

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US5830645A (en) * 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
EP0815121A4 (de) * 1995-02-09 1999-09-01 Univ Washington Streptavidin mit veränderrer affinität
US6072043A (en) * 1996-06-04 2000-06-06 Polyprobe, Inc. Optimally fluorescent oligonucleotides
US5922536A (en) * 1996-10-18 1999-07-13 Nivens; David E. Method for nucleic acid isolation using supercritical fluids
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays
US6130065A (en) * 1997-04-11 2000-10-10 St. Jude Children's Research Hospital Nitrobenzylmercaptopurineriboside (NBMPR)-insensitive, equilibrative, nucleoside transport protein, nucleic acids encoding the same and methods of use
US6077673A (en) * 1998-03-31 2000-06-20 Clontech Laboratories, Inc. Mouse arrays and kits comprising the same
US6277568B1 (en) * 1998-04-09 2001-08-21 Incyte Genomics, Inc. Nucleic acids encoding human ubiquitin-conjugating enzyme homologs
US6268147B1 (en) * 1998-11-02 2001-07-31 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
CN1350591A (zh) * 1999-03-18 2002-05-22 埃克西库恩公司 以单一步骤制备和检测复合生物样品中的核酸
ATE465168T1 (de) * 1999-03-18 2010-05-15 Exiqon As Xylo-lna analoge
US20020051981A1 (en) * 2000-03-08 2002-05-02 Robert Getts Methods for assay and detection on a microarray
WO2002033125A1 (en) * 2000-09-20 2002-04-25 Datascope Investment Corp. Nucleic acid detection by dendrimeric labeling
US6387624B1 (en) * 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing

Also Published As

Publication number Publication date
JP2005502346A (ja) 2005-01-27
EP1438435A4 (de) 2005-11-02
EP1438435B1 (de) 2007-10-31
WO2003020902A2 (en) 2003-03-13
AU2002332778A1 (en) 2003-03-18
DE60223276T2 (de) 2008-08-14
WO2003020902A3 (en) 2003-12-11
EP1438435A2 (de) 2004-07-21
US20040009487A1 (en) 2004-01-15

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Legal Events

Date Code Title Description
8381 Inventor (new situation)

Inventor name: KADUSHIN, JAMES M., MONTVALE, NJ 07645, US

Inventor name: GETTS, ROBERT C., MONTVALE, NJ 07645, US

Inventor name: ZAK, ARIEH S., MONTVALE, NJ 07645, US

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GENISPHERE INC., HAFTFIELD, PA., US

8327 Change in the person/name/address of the patent owner

Owner name: GENISPHERE, LLC., HATFIELD, PA., US