DE60028685D1 - Verfahren zur Klonierung und Expression des Nhe I Restriktionsenzym in E. coli - Google Patents

Verfahren zur Klonierung und Expression des Nhe I Restriktionsenzym in E. coli

Info

Publication number
DE60028685D1
DE60028685D1 DE60028685T DE60028685T DE60028685D1 DE 60028685 D1 DE60028685 D1 DE 60028685D1 DE 60028685 T DE60028685 T DE 60028685T DE 60028685 T DE60028685 T DE 60028685T DE 60028685 D1 DE60028685 D1 DE 60028685D1
Authority
DE
Germany
Prior art keywords
nhe
cloning
coli
expressing
restriction enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60028685T
Other languages
English (en)
Other versions
DE60028685T2 (de
Inventor
Shuang-Yong Xu
Jian-Ing Xiao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of DE60028685D1 publication Critical patent/DE60028685D1/de
Application granted granted Critical
Publication of DE60028685T2 publication Critical patent/DE60028685T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60028685T 1999-10-28 2000-10-26 Verfahren zur Klonierung und Expression des Nhe I Restriktionsenzym in E. coli Expired - Lifetime DE60028685T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US428747 1982-09-30
US09/428,747 US6387681B1 (en) 1999-10-28 1999-10-28 Method for cloning and expression of NHEI restriction endonuclease in E. coli.

Publications (2)

Publication Number Publication Date
DE60028685D1 true DE60028685D1 (de) 2006-07-27
DE60028685T2 DE60028685T2 (de) 2007-05-24

Family

ID=23700240

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60028685T Expired - Lifetime DE60028685T2 (de) 1999-10-28 2000-10-26 Verfahren zur Klonierung und Expression des Nhe I Restriktionsenzym in E. coli

Country Status (4)

Country Link
US (1) US6387681B1 (de)
EP (2) EP1721984A3 (de)
JP (1) JP4719347B2 (de)
DE (1) DE60028685T2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103361329B (zh) * 2007-07-12 2020-03-20 新英格兰生物实验室公司 高保真度限制性内切核酸酶

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200333A (en) 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
US5498535A (en) 1994-05-24 1996-03-12 New England Biolabs, Inc. Method for direct cloning of nuclease genes in E. coli
US5476768A (en) * 1995-03-10 1995-12-19 Becton, Dickinson And Company Mycobacteriophage DSGA specific for the mycobacterium tuberculosis complex
US6383770B1 (en) 1997-09-02 2002-05-07 New England Biolabs, Inc. Method for screening restriction endonucleases
WO1999064632A1 (en) 1998-06-12 1999-12-16 New England Biolabs, Inc. Restriction enzyme gene discovery method

Also Published As

Publication number Publication date
JP4719347B2 (ja) 2011-07-06
EP1096015B1 (de) 2006-06-14
JP2001197889A (ja) 2001-07-24
EP1721984A2 (de) 2006-11-15
EP1721984A3 (de) 2006-12-13
EP1096015A2 (de) 2001-05-02
DE60028685T2 (de) 2007-05-24
US6387681B1 (en) 2002-05-14
EP1096015A3 (de) 2001-05-30

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Legal Events

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8364 No opposition during term of opposition