DE589034C - Process for the preparation of a therapeutically effective preparation - Google Patents

Description

To produce immunity to malignant growths, e.g. B. by pretreatment with Tumor juices, killed or live tumor cells, tumor extracts, and the like, are useful so far not successful. Even if occasionally 'with' tumor vaccinations in animal experiments Immunity could be observed, but it has never been possible to successfully immunize against tumors systematically.

to It has now been found that a highly immunizing against malignant tumors, Safe to use preparation can win if you squeeze tumors or extract them with water or aqueous salt solutions and during further processing at low temperatures, the o ° is not essential may exceed, works. One proceeds z. B. in such a way that you can freshly removed tumor tissue - around the stage of its highest growth potential - at temperatures of about 0 ° or below in their cell structure e.g. through Rub with quartz sand, kieselguhr or other common aids or destroyed by plasmolysis, the resulting pulp with ice-cold water or chilled Diluted salt solutions, the resulting sus-

. pension in the usual way, e.g. by centrifugation and filtration, clarifies and the obtained clear liquid finally z. B. with the help of disinfection filters of malignant Cells or pathogenic germs that cannot be nitrated are freed. That at a temperature which has not exceeded about 0 ° in any work step are extracts obtained Water-clear or yellowish or reddish in color and contain the genuine cell substances. They are either kept at a temperature below 0 ° until they are used, optionally freezing of the solution is not detrimental to the effectiveness of the preparation, or they are im Vacuum dried at about 0 ° in the usual way. The trotkne residue can again to be dissolved or suspended in water to a clear solution, he is also at Can be kept at ordinary temperature.

Just by keeping the low temperature the preservation of the immunizing Effect achieved. If according to the instructions in Hoppe-Seyler's magazine for physiological chemistry, volume 62, 1909, page 148, is worked, one arrives at a product that, as tests have shown, does not meet the aims pursued here, but its immunizing effect is greatly reduced.

example

Very fresh tumor material removed under sterile conditions is included at o °

* ') The patent seeker stated that the inventors were:

Dr. Gerhard Domagk and Dr. Otto Ripke in Wuppertal-Elberfeld.

Quartz sand rubbed in and mixed with ice-cold distilled water in a ratio of ι: S until ι: io shaken. Then it is spun off in refrigerated centrifuges chilled glass filters filtered, centrifuged again and filtered through ice-cooled clarifying filters, finally centrifuged again and filtered through a cooled disinfection filter. All described Operations are to be carried out in the cold. The finished extracts are stored at temperatures below 0 ° until for parenteral injection into the organism to be treated.

Subcutaneous pretreatment with this extract protects against the attack of malignant ones Vaccine tumors.

By drying the extract at 0 ° the result is a dry preparation that can be kept even at normal temperature.

Claims (1)

Claim: Process for the preparation of a therapeutically effective preparation, thereby characterized in that tumors by conventional methods at low temperatures, the o ° is not essential exceed, squeezes or extracted with water or aqueous salt solutions and optionally the available pressed juice or extract cleans and / or for Brings dryness.