DE3887030T2 - Serumfreier Nährboden. - Google Patents

Serumfreier Nährboden.

Info

Publication number
DE3887030T2
DE3887030T2 DE88306022T DE3887030T DE3887030T2 DE 3887030 T2 DE3887030 T2 DE 3887030T2 DE 88306022 T DE88306022 T DE 88306022T DE 3887030 T DE3887030 T DE 3887030T DE 3887030 T2 DE3887030 T2 DE 3887030T2
Authority
DE
Germany
Prior art keywords
serum
culture medium
free culture
free
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE88306022T
Other languages
English (en)
Other versions
DE3887030D1 (de
Inventor
Hideaki Hagiwara
Masafumi Naito
Hideo Yuasa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HAGIWARA HIDEAKI KASAI HYOGO JP
HAGIWARA YOSHIHIDE TAKARAZUKA HYOGO JP
Original Assignee
HAGIWARA HIDEAKI KASAI HYOGO JP
HAGIWARA YOSHIHIDE TAKARAZUKA HYOGO JP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HAGIWARA HIDEAKI KASAI HYOGO JP, HAGIWARA YOSHIHIDE TAKARAZUKA HYOGO JP filed Critical HAGIWARA HIDEAKI KASAI HYOGO JP
Publication of DE3887030D1 publication Critical patent/DE3887030D1/de
Application granted granted Critical
Publication of DE3887030T2 publication Critical patent/DE3887030T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/863—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving IgM
    • Y10S530/864—Monoclonal
    • Y10S530/865—Human
DE88306022T 1987-08-21 1988-07-01 Serumfreier Nährboden. Expired - Fee Related DE3887030T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62206569A JPH0634713B2 (ja) 1987-08-21 1987-08-21 ヒト／ヒト・ハイブリドーマの培養方法

Publications (2)

Publication Number Publication Date
DE3887030D1 DE3887030D1 (de) 1994-02-24
DE3887030T2 true DE3887030T2 (de) 1994-04-28

Family

ID=16525567

Family Applications (1)

Application Number Title Priority Date Filing Date
DE88306022T Expired - Fee Related DE3887030T2 (de) 1987-08-21 1988-07-01 Serumfreier Nährboden.

Country Status (10)

Country Link
US (1) US5155036A (de)
EP (1) EP0304150B1 (de)
JP (1) JPH0634713B2 (de)
KR (1) KR940003651B1 (de)
AU (1) AU615918B2 (de)
CA (1) CA1299509C (de)
DE (1) DE3887030T2 (de)
ES (1) ES2061655T3 (de)
IL (1) IL86830A0 (de)
RU (1) RU2018532C1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2123094C (en) * 1991-11-06 1999-08-10 Paulo N. Correa Cell culture medium
US5846529A (en) * 1993-08-23 1998-12-08 Nexell Therapeutics, Inc. Infusion of neutrophil precursors for treatment of neutropenia
US6037174A (en) * 1993-08-23 2000-03-14 Nexell Therapeutics, Inc. Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
US20030077757A1 (en) * 2000-01-11 2003-04-24 Andrews William H. Method of treating aging-related disorders
JP2006500931A (ja) * 2002-09-30 2006-01-12 ファイザー・プロダクツ・インク 高レベルのヒト配列抗体を産生するハイブリドーマ
CN107099508A (zh) * 2017-06-23 2017-08-29 曲宝赤 一种无血清杂交瘤细胞培养基

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4205126A (en) * 1978-01-01 1980-05-27 Cartaya Oscar A Serum-free cell culture media

Also Published As

Publication number Publication date
EP0304150A3 (en) 1989-08-30
DE3887030D1 (de) 1994-02-24
AU615918B2 (en) 1991-10-17
KR890003944A (ko) 1989-04-19
JPS6451079A (en) 1989-02-27
RU2018532C1 (ru) 1994-08-30
US5155036A (en) 1992-10-13
ES2061655T3 (es) 1994-12-16
CA1299509C (en) 1992-04-28
IL86830A0 (en) 1988-11-30
KR940003651B1 (ko) 1994-04-25
EP0304150A2 (de) 1989-02-22
EP0304150B1 (de) 1994-01-12
JPH0634713B2 (ja) 1994-05-11
AU1842188A (en) 1989-02-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee