DE3671918D1 - Nukleinsaeure sequenzanalyse durch hemmung von exonuklease. - Google Patents

Nukleinsaeure sequenzanalyse durch hemmung von exonuklease.

Info

Publication number
DE3671918D1
DE3671918D1 DE8686904235T DE3671918T DE3671918D1 DE 3671918 D1 DE3671918 D1 DE 3671918D1 DE 8686904235 T DE8686904235 T DE 8686904235T DE 3671918 T DE3671918 T DE 3671918T DE 3671918 D1 DE3671918 D1 DE 3671918D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid sequence
sequence analysis
exonuclease
inhibiting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686904235T
Other languages
English (en)
Inventor
Christopher Mundy
Martin Cunningham
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare Ltd
Original Assignee
Amersham International PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham International PLC filed Critical Amersham International PLC
Application granted granted Critical
Publication of DE3671918D1 publication Critical patent/DE3671918D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE8686904235T 1985-06-17 1986-06-17 Nukleinsaeure sequenzanalyse durch hemmung von exonuklease. Expired - Fee Related DE3671918D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB858515276A GB8515276D0 (en) 1985-06-17 1985-06-17 Nucleic acid sequencing
PCT/GB1986/000349 WO1986007612A1 (en) 1985-06-17 1986-06-17 Nucleic acid sequencing by exonuclease inhibition

Publications (1)

Publication Number Publication Date
DE3671918D1 true DE3671918D1 (de) 1990-07-19

Family

ID=10580855

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686904235T Expired - Fee Related DE3671918D1 (de) 1985-06-17 1986-06-17 Nukleinsaeure sequenzanalyse durch hemmung von exonuklease.

Country Status (5)

Country Link
EP (1) EP0227772B1 (de)
JP (1) JPS63500006A (de)
DE (1) DE3671918D1 (de)
GB (1) GB8515276D0 (de)
WO (1) WO1986007612A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
EP0348458B1 (de) 1987-11-30 1997-04-09 University Of Iowa Research Foundation Durch modifikationen an der 3'-terminalen phosphodiesterbindung stabilisierte dna moleküle, ihre verwendung als nukleinsäuresonden sowie als therapeutische mittel zur hemmung der expression spezifischer zielgene
DE3930312A1 (de) * 1988-10-24 1990-04-26 Max Planck Gesellschaft Verfahren zur direkten sequenzierung von nukleinsaeureketten
FR2649122B1 (fr) * 1989-07-03 1991-10-18 Pasteur Institut Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU8997991A (en) * 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
JP2531053Y2 (ja) * 1991-03-29 1997-04-02 大倉工業株式会社 収録用品
ES2112302T3 (es) * 1991-10-11 1998-04-01 Behringwerke Ag Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos.
JPH0630799A (ja) * 1992-07-10 1994-02-08 Hitachi Ltd 遺伝子検出方法及び遺伝子検出装置
WO1995006752A1 (en) * 1993-09-03 1995-03-09 Duke University A method of nucleic acid sequencing
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
JP2001524808A (ja) 1996-12-10 2001-12-04 ジーントレイス・システムズ・インコーポレイテッド 放出可能な不揮発性の質量標識分子
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US7270958B2 (en) 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
GB8311018D0 (en) * 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna

Also Published As

Publication number Publication date
GB8515276D0 (en) 1985-07-17
WO1986007612A1 (en) 1986-12-31
EP0227772B1 (de) 1990-06-13
EP0227772A1 (de) 1987-07-08
JPS63500006A (ja) 1988-01-07

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee