DE3667292D1 - Markierung von oligonukleotiden. - Google Patents

Markierung von oligonukleotiden.

Info

Publication number
DE3667292D1
DE3667292D1 DE8686102766T DE3667292T DE3667292D1 DE 3667292 D1 DE3667292 D1 DE 3667292D1 DE 8686102766 T DE8686102766 T DE 8686102766T DE 3667292 T DE3667292 T DE 3667292T DE 3667292 D1 DE3667292 D1 DE 3667292D1
Authority
DE
Germany
Prior art keywords
oligonucleotids
marking
marking oligonucleotids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686102766T
Other languages
English (en)
Inventor
Nanibhushan Dr Dattagupta
William Dr Knowles
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Diagnostics Inc
Original Assignee
Molecular Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/712,481 external-priority patent/US4808520A/en
Application filed by Molecular Diagnostics Inc filed Critical Molecular Diagnostics Inc
Application granted granted Critical
Publication of DE3667292D1 publication Critical patent/DE3667292D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE8686102766T 1985-03-15 1986-03-03 Markierung von oligonukleotiden. Expired - Fee Related DE3667292D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/712,481 US4808520A (en) 1985-03-15 1985-03-15 Labelling of oligonucleotides
US80887185A 1985-12-18 1985-12-18

Publications (1)

Publication Number Publication Date
DE3667292D1 true DE3667292D1 (de) 1990-01-11

Family

ID=27108843

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686102766T Expired - Fee Related DE3667292D1 (de) 1985-03-15 1986-03-03 Markierung von oligonukleotiden.

Country Status (10)

Country Link
EP (1) EP0194545B1 (de)
AU (1) AU592777B2 (de)
CA (1) CA1278755C (de)
DE (1) DE3667292D1 (de)
DK (1) DK119086A (de)
ES (1) ES8705920A1 (de)
FI (1) FI861039A (de)
IL (1) IL78123A (de)
NO (1) NO860710L (de)
NZ (1) NZ215453A (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5604099A (en) * 1986-03-13 1997-02-18 Hoffmann-La Roche Inc. Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
US5047523A (en) * 1988-08-02 1991-09-10 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of neisseria gonorrhoea
US4879214A (en) * 1988-11-15 1989-11-07 E. I. Du Pont De Nemours And Company Differentiation of nucleic acid segments on the basis of nucleotide differences
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
CA2005927A1 (en) * 1988-12-21 1990-06-21 Chander Bahl Method of preparing nucleotide probes using a bridging complement
JPH03123500A (ja) * 1989-10-06 1991-05-27 Canon Inc 核酸の分別方法
DE69223755T2 (de) 1991-10-11 1998-04-23 Behringwerke Ag Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung
US5612199A (en) * 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
GB9201073D0 (en) * 1992-01-18 1992-03-11 Cytocell Ltd Chromosome hybridisation methods
WO1994003637A1 (en) * 1992-07-31 1994-02-17 Syntex (Usa) Inc. Method for introducing defined sequences at the 3' end of polynucleotides
US6294323B1 (en) 1993-04-14 2001-09-25 Behringwerke Ag Self initiating single primer amplification of nucleic acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1254525A (en) * 1983-04-13 1989-05-23 Christine L. Brakel Kit for terminally chemically labeling dna
DE3441369A1 (de) * 1984-11-13 1986-05-22 Bayer Ag, 5090 Leverkusen Verfahren zur herstellung von hydroxymethylen-alkoxyessigsaeureestern
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
CA1272443A (en) * 1985-02-22 1990-08-07 Nanibhushan Dattagupta Solution-phase dual hybridization assay for detecting polynucleotide sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve

Also Published As

Publication number Publication date
ES553034A0 (es) 1987-05-16
DK119086A (da) 1986-09-16
FI861039A (fi) 1986-09-16
IL78123A0 (en) 1986-07-31
AU5443186A (en) 1986-09-18
AU592777B2 (en) 1990-01-25
EP0194545B1 (de) 1989-12-06
ES8705920A1 (es) 1987-05-16
FI861039A0 (fi) 1986-03-13
CA1278755C (en) 1991-01-08
DK119086D0 (da) 1986-03-14
NO860710L (no) 1986-09-16
EP0194545A2 (de) 1986-09-17
EP0194545A3 (en) 1988-01-13
NZ215453A (en) 1989-06-28
IL78123A (en) 1990-02-09

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee