DE3631958A1 - Elektrophorese-vorrichtung fuer die sequenzanalyse von nukleinsaeuren - Google Patents
Elektrophorese-vorrichtung fuer die sequenzanalyse von nukleinsaeurenInfo
- Publication number
- DE3631958A1 DE3631958A1 DE19863631958 DE3631958A DE3631958A1 DE 3631958 A1 DE3631958 A1 DE 3631958A1 DE 19863631958 DE19863631958 DE 19863631958 DE 3631958 A DE3631958 A DE 3631958A DE 3631958 A1 DE3631958 A1 DE 3631958A1
- Authority
- DE
- Germany
- Prior art keywords
- glass plate
- flat
- plate
- gel
- plastic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001962 electrophoresis Methods 0.000 title claims description 9
- 238000012300 Sequence Analysis Methods 0.000 title description 5
- 150000007523 nucleic acids Chemical class 0.000 title description 3
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 239000011521 glass Substances 0.000 claims description 74
- 239000004033 plastic Substances 0.000 claims description 42
- 229920003023 plastic Polymers 0.000 claims description 42
- 125000006850 spacer group Chemical group 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- 239000000853 adhesive Substances 0.000 claims description 11
- 230000001070 adhesive effect Effects 0.000 claims description 11
- 229920001971 elastomer Polymers 0.000 claims description 9
- 239000000806 elastomer Substances 0.000 claims description 8
- 230000014759 maintenance of location Effects 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000005357 flat glass Substances 0.000 claims 7
- 239000000499 gel Substances 0.000 description 38
- 239000007853 buffer solution Substances 0.000 description 17
- 241000234295 Musa Species 0.000 description 5
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 3
- 229920002379 silicone rubber Polymers 0.000 description 3
- 239000004945 silicone rubber Substances 0.000 description 3
- 239000002184 metal Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 241001417935 Platycephalidae Species 0.000 description 1
- 229920005830 Polyurethane Foam Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003522 acrylic cement Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000007688 edging Methods 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 239000013536 elastomeric material Substances 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000000497 foam cell Anatomy 0.000 description 1
- 229920001821 foam rubber Polymers 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 239000002984 plastic foam Substances 0.000 description 1
- 239000011496 polyurethane foam Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000013464 silicone adhesive Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 210000002105 tongue Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Securing Of Glass Panes Or The Like (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrostatic Separation (AREA)
- Joining Of Glass To Other Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/782,756 US4663015A (en) | 1985-09-30 | 1985-09-30 | Electrophoresis apparatus for nucleic acid sequencing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3631958A1 true DE3631958A1 (de) | 1987-04-02 |
| DE3631958C2 DE3631958C2 (enExample) | 1990-10-31 |
Family
ID=25127084
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19863631958 Granted DE3631958A1 (de) | 1985-09-30 | 1986-09-19 | Elektrophorese-vorrichtung fuer die sequenzanalyse von nukleinsaeuren |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4663015A (enExample) |
| JP (1) | JPH0664010B2 (enExample) |
| CA (1) | CA1296294C (enExample) |
| DE (1) | DE3631958A1 (enExample) |
| GB (1) | GB2181248B (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USD303012S (en) | 1987-02-02 | 1989-08-22 | Life Technologies, Inc. | Electrophoresis sequencing apparatus tray |
| USD303007S (en) | 1987-02-02 | 1989-08-22 | Life Technologies, Inc. | Electrophoresis sequencing apparatus |
| US4929329A (en) * | 1987-04-27 | 1990-05-29 | Eg&G, Inc. | Electrophoresis cassette system with apparatus and method for filling same |
| US4919784A (en) * | 1989-08-16 | 1990-04-24 | Eg&G, Inc. | Electrophorresis cassette spacer |
| US5073246A (en) * | 1990-05-16 | 1991-12-17 | Bio-Rad Laboratories, Inc. | Slab electrophoresis system with improved sample wells and cooling mechanism |
| US5167784A (en) * | 1991-09-04 | 1992-12-01 | Xerox Corporation | Sequencing large nucleic acid fragments |
| USD368143S (en) | 1994-05-24 | 1996-03-19 | Li-Cor, Inc. | DNA sequencing cabinet |
| US5792332A (en) * | 1995-12-21 | 1998-08-11 | Hoefer Pharmacia Biotech, Inc. | Safe electrophoresis unit |
| US5656145A (en) * | 1996-05-20 | 1997-08-12 | Bio-Rad Laboratories, Inc. | Needle guide for loading samples into a vertical slab gel |
| US5882495A (en) * | 1996-11-12 | 1999-03-16 | Proteome, Inc. | Electrophoresis system |
| US5993628A (en) * | 1998-04-17 | 1999-11-30 | The Perkin-Elmer Corporation | Electrophoresis method and apparatus for separating bio-organic molecules |
| US6632340B2 (en) * | 2001-03-23 | 2003-10-14 | Bio-Rad Laboratories, Inc. | Precast gel and tray combination for submerged gel electrophoresis |
| US7214300B2 (en) * | 2001-06-04 | 2007-05-08 | Epocal Inc. | Integrated electrokinetic devices and methods of manufacture |
| US9234874B2 (en) * | 2011-02-24 | 2016-01-12 | Bio-Rad Laboratories, Inc. | Dimensional stabilization of slab gel cassettes to prevent distortion caused by swelling gels |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3719580A (en) * | 1971-06-04 | 1973-03-06 | R Roberts | Electrophoretic apparatus |
| US4035377A (en) * | 1975-06-23 | 1977-07-12 | Cold Spring Harbor Laboratory | Slab gel electrophoresis apparatus |
| US4339327A (en) * | 1981-04-23 | 1982-07-13 | Tyler Jonathan M | Miniature slab gel electrophoresis system |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4337131A (en) * | 1978-11-13 | 1982-06-29 | C. Desaga Gmbh Nachf. Erich Fecht | Process for electrophoresis |
| US4560459A (en) * | 1984-02-21 | 1985-12-24 | Hoefer Scientific Instruments | Vertical gel sandwich for use in electrophoresis and method therefor |
| US4518476A (en) * | 1984-04-17 | 1985-05-21 | Bio-Rad Laboratories, Inc. | End clamp for gel slab plate assembly |
| US4574040A (en) * | 1984-04-17 | 1986-03-04 | Bio-Rad Laboratories, Inc. | Apparatus for vertical gel electrophoresis |
| JPH0634684Y2 (ja) * | 1985-06-03 | 1994-09-07 | 寶酒造株式会社 | 冷却式電気泳動装置 |
-
1985
- 1985-09-30 US US06/782,756 patent/US4663015A/en not_active Expired - Lifetime
-
1986
- 1986-06-24 GB GB8615341A patent/GB2181248B/en not_active Expired
- 1986-07-04 CA CA000513166A patent/CA1296294C/en not_active Expired - Lifetime
- 1986-09-08 JP JP61211347A patent/JPH0664010B2/ja not_active Expired - Lifetime
- 1986-09-19 DE DE19863631958 patent/DE3631958A1/de active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3719580A (en) * | 1971-06-04 | 1973-03-06 | R Roberts | Electrophoretic apparatus |
| US4035377A (en) * | 1975-06-23 | 1977-07-12 | Cold Spring Harbor Laboratory | Slab gel electrophoresis apparatus |
| US4339327A (en) * | 1981-04-23 | 1982-07-13 | Tyler Jonathan M | Miniature slab gel electrophoresis system |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3631958C2 (enExample) | 1990-10-31 |
| JPS6281558A (ja) | 1987-04-15 |
| GB2181248A (en) | 1987-04-15 |
| CA1296294C (en) | 1992-02-25 |
| GB2181248B (en) | 1989-11-15 |
| US4663015A (en) | 1987-05-05 |
| GB8615341D0 (en) | 1986-07-30 |
| JPH0664010B2 (ja) | 1994-08-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |