DE3508875A1 - Method and product for stimulating the immune system - Google Patents

Abstract

Constituents of oil-producing plants are employed in defined dosage for stimulating the immune system of living humans and animals. At this dosage, the constituents bring about stimulation but no cell damage.

Description

Preparation for stimulating the immune system

The invention relates to a pharmaceutical preparation for stimulation of the immune system of living human and animal organisms.

The inventive preparation is characterized in that as ingredients Black pepper oil, cinnamon blossom oil, cardamom oil, linallyl acetate are used, Cinnamaldehyde, safrole, carvone, cis / trans citral, or a mixture of the following essential oils in the alcoholic distillate of 536 mg Folia Melissae, 714 mg Rhizoma Helenii, 714 mg Radix Angelicae, 714 mg Rhizoma Zingiberis, 285 mg Flores Caryophyili, 285 mg Rhizoma Galangae, 714 mg Radix Gentianae, 321 mg Cortex Chinnamomae and 71.4 mg Fructus Piperis nigri (= so-called special mixture) or mixtures of the above Ingredients and that one or more of these ingredients in a pharmaceutical Application vehicle are mixed in.

The invention is based on the knowledge that the immune system is more alive Human and animal organisms can be stimulated through use in organic Solvent soluble ingredients of essential oil drugs, if these ingredients administered in daily doses of 10 to 1000 mg (milligrams), preferably 5 to 100 mg, on 70 kg (kilograms) weight of the living organism and that the Daily doses are administered in a period which does not include more days than the twice the number of daily doses to be administered.

These ingredients show an immune system stimulating effect when used at a concentration one or more powers of ten lower lies than that Concentration at which these ingredients show toxic effects on living cells.

Since these ingredients can be obtained from essential oil drugs, which has proven itself for human and animal nutrition for many years and have been shown to be harmless in the dosage in question is also to be expected that the amounts of the ingredients to be used according to the invention do not cause any serious harmful side effect.

It is recommended, depending on the circumstances, to treat that the daily doses are administered on consecutive days, or in such a way that the administration of the daily doses with the inclusion of break days, on which no ingredients are administered and that the break days are closed one to three approximately equally long pauses are grouped together evenly over are spread over the period of treatment.

Continue with page 4 of the documents originally submitted.

Examples of the administration of the daily doses in the treatment period result from the table below.

TABLE 1 number of length of numbering numbering of daily doses action time of the days in days of the treatment room in days within the treatment period, Treatments in which there are no periods of time and daily doses doses are administered (break days) (dose days) 10 10 1st - 10th

20 40 1. - 10. 11. - 30.

31st - 40th

30 30 1st - 30th

30 40 1st - 15th 16th - 25th

26th - 40th

30 45 1. - 10. 11. - 20.

21. - 30. 31. - 35.

36th - 45th

30 50 1. - 10. 11. - 20.

21. - 30. 31. - 40.

41-50

35 35 1st - 35th

40 40 1st - 40th

50 90 1. - 10. 11. - 20.

21. - 30. 31. - 40.

41st - 50th 51st - 60th

61. - 70. 71. - 80.

81-90

Preference is given to using ingredients which have been obtained by steam distillation can be obtained from essential oil drugs.

The ingredients black pepper oil, cinnamon blossom oil, Cardamom oil, linallyl acetate, cinnamaldehyde, safrole, carvone and cis / trans citral.

In addition, a mixture of the following has proven itself as an ingredient essential oils in the alcoholic distillate of 536 mg Folia Melissae, 714 mg Rhizoma Helenii, 714 mg Radix Angelicae, 714 mg Rhizoma Zingiberis, 285 mg Flores Caryophylli, 285 mg Rhizoma Galangae, 714 mg Radix Gentianae, 321 mg Cortex Cinnamomae and 71.4 mg of Fructus Piperis nigri. This mixture is referred to below as the special mixture.

Mixtures of the ingredients mentioned have also proven useful including the special mix.

A pharmaceutical preparation according to the invention, that is to say for stimulation of the immune system of living human and animal organisms, is produced by using one or more of these ingredients by steam distillation Dissolve with an organic solvent, for example water or alcohol, or by pressing out of those essential oil drugs in which the relevant Ingredients are contained, extracted and then converted into a pharmaceutical vehicle mixed in in a mixing ratio of 0.2: 100 to 40: 100.

The ingredients listed below can be derived from essential oil drugs can be obtained by steam distillation as follows: Black pepper oil from the fruit grains of Piper nigrum; Cinnamon blossom oil from the flowers of the cinnamonum Cassia; Cardamom oil made from the seeds of Elettaria cardamomum; Linallyl acetate from the Flowers of lavandula; Cinnamaldehyde from the bark of Cinnamonum ceylanicum; Safrole from the root of the Sassafrasi Carvone from the fruit of the Why carvi and cis / trans Citral from the leaves of the Cymbopogon citratus.

A pharmaceutical preparation according to the invention, that is to say for stimulation of the immune system of living human and animal organisms, consists of one or more of the ingredients that make up a pharmaceutical vehicle are mixed in in a mixing ratio of 0.2: 100 to 40: 100.

The effect that can be achieved with the invention is through comparative experiments occupied as follows.

Experiments were carried out on rabbits, which the corresponding Ingredients received in the drinking water for 42 consecutive days, namely a first group of animals in a daily dose of 5 mg to 70 kg live weight and a second group of animals in a daily dose from 50 mg to 70 kg live weight.

At the end of the 42 days, the animals were infected with a herpes PSR virus infected. A control group of animals that had not been pretreated had one Lethality of 95%, i.e. a survival rate of 5.

Rabbits were treated with cinnamaldehyde and black pepper oil and with a special mixture.

The rabbits pretreated with cinnamaldehyde had a survival rate of 70%, the rabbits pretreated with the special mixture had a survival rate of 33% and the rabbits pretreated with black pepper oil had a survival rate of 17%.

During the experiments, the immune parameters of the treated animals continuously checked. This showed after infection in the pretreated animals an increase in immune parameters, sometimes up to the 40th day after infection a doubling compared to the primary values. This increase in immune parameters was not observed in the animals that had not been pretreated. In addition to the lethality reduction if the pretreatment leads to an increase in immune responses, whereby a stimulation achieved by the pretreatment with the ingredients the immune system is proven.

The experiments were repeated on rats and showed the same Results.

From the permanent strains of the cell types "Girardi Heart" (GH), "Flow Cell cultures were set up in 12000 "(FL)," Intestine 407 "(IN) and glVero Kidney" (VE) and treated with one ingredient each in different doses. the detrimental dosage was shown by cells clumping together. The harmless ones Dosages are given in TABiLLs 2.

The table shows that the dosage used in the animal experiments is far below that which is harmful to living cells.

TABLE 2 Ingredient Treated Range of Virucidal Concentration, in which no cell substance cell damage was observed, based on mg of the ingredient on every 10 kg of treated cell substance black pepper oil (Oleum Piperis nigri) GH up to 0.1 to 0.1 IN up to 1 "VE up to 0.1 Cinnamon blossom oil CU up to 0.1 (Oleum Cassiae flores) GH to 0.1 to 0.1 IN to 0.1 VE to 0.1 Cardamom oil CH to 1 (Oleum Cardamomi) FL up to 1 IN up to 1 VE up to 10 Linallylacetat GH up to 0.1 FL up to 1 IN up to 1 VE up to 1 cinnamaldehyde GH up to 1 FL up to 1 IN up to 1 VE up to 1 Safrol GH up to 1 FL FL up to 1 IN up to 10 PU up to 1 carvone GH up to 1 FL up to 1 "IN up to 1 PU up to 1 TABEL 1 - Continuation of the ingredient treated area of the virucidal Kon cell substance concentration, in which no cell damage was observed, in mg of each ingredient 10 kg of treated cell substance cis / trans Citral GH up to 1 FL up to 1 IN up to 1 VE up to 1 Example 1 (solution for injection) 100 g (grams) black pepper oil are dissolved in 2 1 (liter) 1,2-propanediol. The solution is at 121ob (Celsius) sterilized in the autoclave for 50 minutes, then cooled and added in portions 2 g filled into ampoules.

One ampoule contains 10 mg black pepper oil and contains an average daily dose for a 70 kg adult to stimulate the immune system. This daily dose is used for 20 to 45 consecutive days. The mixing ratio of the ingredient used for 1,2-propanediol is 5 in this example: 100; other mixing ratios for injection solutions are also possible, and although in the range between 0.4: 100 and 10: 100, you must, however, the daily dose of the Injection solution then the modified content of the ingredient of the injection solution adjust.

Example 2 (solution for injection) As Example 1 with the only difference that 100 g of cinnamaldehyde are used instead of 100 g of black pepper oil.

Example 3 (solution for injection) As Example 1 with the only difference that 100 g of the special mixture are used instead of 100 g of black pepper oil.

Example 4 (Aerosol) 650 g of black pepper oil are dissolved in 631 g of ether mixed with 1,805 g of ethanol dissolved. 31 g esters of castor oil fatty acids are added to this solution mixed in with oxyethylated glycerine and 210 g of caprylic / capric acid triglyceride. 0.536 g of this mixture are used together with 0.505 g of difluoro-dichloromethane as a propellant bottled in spray cans. The spray can are sealed and have a metering valve on, which releases a predetermined portion of the mixture with each actuation, the is then sprayed under the pressure of the difluorochloromethane as an aerosol.

By setting and dimensioning the metering valve accordingly ensured that with each actuation a single dose of 5.0 mg black pepper oil is released.

Aerosol is put into the mouth for therapy and prophylaxis of influenza infections or nose sprayed and inhaled. For an adult weighing 70 kg, pro Day ten such spray portions with a total of 1C x 5 mg = 50 mg black pepper oil a beneficial treatment.

The mixing ratio of the ingredient used to the aerosol substance in this example is 24: 10C; there are also other mixing ratios for the aerosol is possible, in the range between 2: 100 to 40: 100, you have to however, the daily dose is then the modified content of the ingredient of each spray portion adjust.

Example 5 (capsule) A capsule filling is produced from 50 g cinnamaldehyde and 3 g soy lecithin as an emulsifier. Each capsule contains 53 mg of this Ka? Selfillung and is enclosed in a capsule shell made from 87.5 mg gelatin and 37.5 consists of glycerin.

Such a capsule is taken orally to stimulate the immune system Day administered to adult patients weighing 70 kg, during 20 to 45 consecutive days.

One such capsule contains 50 mg of cinnamaldehyde. There are others too Capsule fillings possible, in the range between 1 and 100 mg, but you have to then adjust the daily dose to the changed content of the ingredient.

Example 6 (capsule) As Example 5 with the only difference that instead of 50 g of cinnamaldehyde, 50 g of black pepper oil are used.

Example 7 (capsule) As Example 5 with the only difference that instead of 50 g of cinnamaldehyde, 50 g of the special mixture can be used.

Example 8 (ointment) 3.0 g paraffinum durum and 87.0 g white petrolatum are heated to 60 ° C and stirred. Add 20 g of cinnamon blossom oil to the warm mixture mixed in. The mixture is cooled and can be used as an ointment for topical use be used. About 0.1 ml of the ointment - which contains about 10 mg of the ingredient - will be used Spread over 50 cm2 skin area. This can be repeated 8 times a day.

The mixing ratio of the ingredient used to the mixture from Paraffinum durum and white Vaseline is in this example 22: 100; it Other mixing ratios for the ointment are also possible, in the range between 2: 100 to 40: 100.

Claims (5)

Claims: 1. Pharmaceutical preparation for stimulating the Immune system of living human and animal organisms, characterized in that that black pepper oil, cinnamon blossom oil, cardamom oil are used as ingredients, Linallyl acetate, cinnamaldehyde, safrole, carvone, cis / trans citral, or a mixture the following essential oils in the alcoholic distillate of 536 mg Folia Melissae, 714 mg Rhizoma Helenii, 714 mg Radix Angelicae, 714 mg Rhizoma Zingiberis, 285 mg Flores Caryophylli, 285 mg Rhizoma Galangae, 714 mg Radix Gentianae, 321 mg Cortex Cinnamomae and 71.4 mg Fructus Piperis nigri (= so-called special mixture) or mixtures from the ingredients mentioned, and that one or more of these ingredients are mixed into a pharmaceutical vehicle. 2. Pharmaceutical injection solution according to claim 1, characterized in that that one or more of the ingredients in 1,2-propanediol in the mixing ratio 0.4: 100 to 10: 100, preferably in a mixing ratio of 5: 100, are dissolved. 3. Pharmaceutical aerosol according to claim 1, characterized in that that one or more of the ingredients in an aerosol substance in the mixing ratio 2: 100 to 40: 100, preferably in a mixing ratio of 24: 100 and that the aerosol substance from 1 part of ether, 2 to 5 parts of ethanol, 0.02 to 0.1 parts of esters of castor oil fatty acids with ethoxylated glycerol and 0.2 to Part 1 Caprylic / capric acid triglyceride is made and under the propellant gas pressure from 2 to 6 parts of difluoro-dichloromethane. 4. Pharmaceutical, ingestible capsule according to claim 1, characterized in that that a capsule shell is provided which completely encloses a capsule filling and that the capsule filling consists of 1 to 100 mg, preferably 50 mg, of one or more the ingredients are mixed with 1 to 8 mg soy lecithin as an emulsifier and, that the capsule shell is made of gelatine and glycerine in a mixing ratio of 3: 1 to 2 : 1 and that the total weight of the capsule is 80 mg to 200 mg. 5. Pharmaceutical ointment according to claim 1, characterized in that that one or more of the ingredients in a mixing ratio of 2: 100 to 40: 100, preferably in a mixing ratio of 22: 100, are mixed into the ointment vehicle, and that the ointment carrier mass of paraffinum durum mixed with white petroleum jelly in a mixing ratio of 1:20 to 1:35.