DE3069608D1 - A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay - Google Patents

A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay

Info

Publication number
DE3069608D1
DE3069608D1 DE8080107733T DE3069608T DE3069608D1 DE 3069608 D1 DE3069608 D1 DE 3069608D1 DE 8080107733 T DE8080107733 T DE 8080107733T DE 3069608 T DE3069608 T DE 3069608T DE 3069608 D1 DE3069608 D1 DE 3069608D1
Authority
DE
Germany
Prior art keywords
coenzyme
clinical assay
enzyme
solution
aqueous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8080107733T
Other languages
German (de)
English (en)
Inventor
Ivan Endre Modrovich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE3069608D1 publication Critical patent/DE3069608D1/de
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE8080107733T 1980-02-13 1980-12-08 A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay Expired DE3069608D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/121,225 US4394449A (en) 1980-02-13 1980-02-13 Stabilization of coenzymes in aqueous solution

Publications (1)

Publication Number Publication Date
DE3069608D1 true DE3069608D1 (en) 1984-12-13

Family

ID=22395341

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8080107733T Expired DE3069608D1 (en) 1980-02-13 1980-12-08 A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay

Country Status (6)

Country Link
US (1) US4394449A (OSRAM)
EP (1) EP0034213B1 (OSRAM)
JP (1) JPS56124395A (OSRAM)
AT (1) ATE10208T1 (OSRAM)
CA (1) CA1178875A (OSRAM)
DE (1) DE3069608D1 (OSRAM)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3044454C2 (de) * 1980-11-26 1982-12-09 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisierte Enzymzubereitung
US4578265A (en) * 1981-08-13 1986-03-25 Laclede Professional Products, Inc. Di-enzymatic dentifrice
AU574646B2 (en) * 1982-07-19 1988-07-14 Cooperbiomedical Inc. Enzyme assay method
DE3236388A1 (de) * 1982-10-01 1984-04-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur selektiven herstellung reduzierter sauerstoffspezies und hierfuer geeignete reagentien
US4657854A (en) * 1983-05-05 1987-04-14 Ivan Endre Modrovich Assay systems based on magnesium-responsive enzymes
US4622294A (en) * 1985-02-08 1986-11-11 Kung Viola T Liposome immunoassay reagent and method
JPH0673475B2 (ja) * 1985-04-26 1994-09-21 オリエンタル酵母工業株式会社 イソクエン酸脱水素酵素の反応停止法
US5258286A (en) * 1985-07-02 1993-11-02 Oriental Yeast Co., Ltd. Method of terminating isocitrate dehydrogenase reaction
EP0227073A3 (en) * 1985-12-23 1989-03-22 F. Hoffmann-La Roche Ag Method of determining a co-enzyme
US4897346A (en) * 1986-07-15 1990-01-30 Beckman Instruments, Inc. Stabilized liquid enzyme composition for glucose determination
US4940659A (en) * 1987-02-20 1990-07-10 Monoclonetics International, Inc. Screening extra-cellular body fluids for superoxide dismutase (SOD-1) for determining fetal trisomy 21 down syndrome
US5384247A (en) * 1987-04-10 1995-01-24 Boehringer Mannheim, Gmbh Determination of sodium ions in fluids
GB8710882D0 (en) * 1987-05-08 1987-06-10 Health Lab Service Board Estimation of reduced pyridine nucleotides
EP0415188B1 (en) * 1989-08-28 1996-03-20 F. Hoffmann-La Roche Ag Enzymatic method for determining analyte concentrations
EP0463755A1 (en) * 1990-06-27 1992-01-02 INSTRUMENTATION LABORATORY S.p.A. Stable aqueous NADH reagent and kit
US5116728A (en) * 1990-07-20 1992-05-26 Em Diagnostic Systems, Incorporated Reagents for co2 detection
CA2111168A1 (en) * 1992-12-21 1994-06-22 Shing F. Kwan Stable single liquid reagent for the determination of carbon dioxide in serum
AU682882B2 (en) * 1993-09-17 1997-10-23 Thermo Trace Ltd. Reagent
EP0719345B1 (en) * 1993-09-17 2000-09-27 Trace Scientific Limited Reagent for enzymatic determination of serum bicarbonate levels
AUPN200695A0 (en) * 1995-03-28 1995-04-27 Trace Scientific Pty Ltd Transaminase reagent
AU710552B2 (en) * 1995-03-28 1999-09-23 Thermo Trace Ltd. Reagent stabilized using coenzyme reduction system
ES2306453T3 (es) * 1995-12-27 2008-11-01 Asahi Kasei Pharma Corporation Metodo para ensayar una muestra biologica.
JP3674015B2 (ja) * 1996-10-02 2005-07-20 日東紡績株式会社 尿素窒素の測定方法およびその測定用キット
US5801006A (en) * 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
EP1425585A2 (en) * 2001-09-11 2004-06-09 MERCK PATENT GmbH Lateral flow test format for enzyme assays
US6703216B2 (en) 2002-03-14 2004-03-09 The Regents Of The University Of California Methods, compositions and apparatuses for detection of gamma-hydroxybutyric acid (GHB)
CN100359008C (zh) * 2003-09-05 2008-01-02 上海丰汇医学科技有限公司 酶法测定病人分析物浓度用的试剂
ITMI20060629A1 (it) * 2006-03-31 2007-10-01 Daniele Giovannone Composizioni solide orali a base di s-adenosilmetionina e processo per il loro ottenimento
CN104458617A (zh) * 2014-12-05 2015-03-25 重庆中元生物技术有限公司 一种α-羟丁酸脱氢酶检测试剂盒及其制备方法
BR112021014997A2 (pt) * 2019-01-31 2021-10-05 F. Hoffmann-La Roche Ag Kit de ensaio para determinar quantitativamente a concentração de amônia em uma amostra líquida aquosa, método para fornecer um kit de ensaio, uso de um kit de ensaio, kit de ensaio, mistura e dispositivo
WO2025211067A1 (ja) * 2024-04-04 2025-10-09 株式会社シノテスト 補酵素を含む測定試薬及びその安定化方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3413198A (en) * 1966-06-30 1968-11-26 Calbiochem Reagents and method for assaying biological samples
US3776900A (en) * 1971-04-26 1973-12-04 Searle & Co Stabilization of reduced coenzymes
FR2217296A1 (en) * 1972-10-10 1974-09-06 Inst Francais Du Petrole Organic cpds. prepn. by co-enzymatic reduction - with continuous regeneration of the oxidised coenzyme with a reducing agent
AT339505B (de) * 1974-03-14 1977-10-25 Boehringer Mannheim Gmbh Enzymatisches analysenverfahren
NL7603588A (nl) * 1975-04-21 1976-10-25 Hoffmann La Roche Gestabiliseerde coenzymoplossing.
JPS5826320B2 (ja) * 1975-12-27 1983-06-02 中外製薬株式会社 ソクテイヨウシヤクソセイブツ
FR2344570A1 (fr) * 1976-03-17 1977-10-14 Modrovich Ivan Procede de stabilisation d'un coenzyme labile
US4153511A (en) * 1976-03-17 1979-05-08 Modrovich Ivan Endre Stabilized liquid coenzyme compositions
US4277562A (en) * 1976-09-13 1981-07-07 Modrovich Ivan Endre Stabilized liquid enzyme and coenzyme compositions
JPS53107486A (en) * 1977-02-28 1978-09-19 Wako Pure Chem Ind Ltd Stabilized nadh aqueous solution
US4189536A (en) * 1977-11-09 1980-02-19 Hycel, Inc. Reagent systems, enzymatic assays and compositions therefor
DE2831580C2 (de) * 1978-07-18 1980-09-18 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Reagens zur Bestimmung von Glycerin
CA1187388A (en) * 1978-09-20 1985-05-21 American Monitor Corporation Stabilization of working reagent solutions containing nadh, nadph, and/or enzymes, and the use of such stabilized reagents in enzymes or substrate assays
US4218536A (en) * 1978-10-03 1980-08-19 Jonas Maurukas Process-stable co-enzyme NAD solution
US4247633A (en) * 1979-04-05 1981-01-27 Pabst Brewing Company Reagent for colorimetric determination of creative phosphokinase

Also Published As

Publication number Publication date
US4394449A (en) 1983-07-19
ATE10208T1 (de) 1984-11-15
CA1178875A (en) 1984-12-04
EP0034213A1 (en) 1981-08-26
JPS56124395A (en) 1981-09-30
JPS6335238B2 (OSRAM) 1988-07-14
EP0034213B1 (en) 1984-11-07

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8339 Ceased/non-payment of the annual fee